1,723,838 research outputs found
Tulostoma morenoi V. Vlasenko & A. Vlasenko 2023, sp. nov.
<i>Tulostoma morenoi</i> V. Vlasenko & A. Vlasenko, <i>sp. nov.</i> Figs. 1, 2. <p>MycoBank: MB847495.</p> <p> <b>Description:</b> —Spore-sac subglobose, 9 mm wide, 7 mm tall. Exoperidium indistinct, incrusting sand, remaining as a cup-like structure at the base of the spore-sac. Endoperidium thin, smooth, slightly creamy-brownish to grayish white. Mouth oval, indistinct. Socket inconspicuous. Stem slender, 2–3.5 mm wide, 30–35 mm tall, slightly creamy-brownish to grayish white, somewhat longitudinally furrowed to fissured and appressed scaly closer to the spore-sac, with a small basal mycelial bulb. Mature gleba orange brown. Capillitial threads (1.6)3.5–4(6.3) µm wide, hyaline, undulating, irregular thickness, thick-walled, with uneven inner walls, ramified. Septa rare, slightly widened up to strongly widened. Spores subglobose, regularly verrucose, 5.6–8.5 × 6.6–9.2 μm without ornamentation, 6.2–9.8 × 6.8–10.6 μm including warts. Warts are large, 6–7 on visible side of spore, 0.5–0.6 μm wide, 0.8–0.9 μm length, more or less isolated or merging into groups with small warts or forming ridge-like anastomoses at the base, visible on SEM.</p> <p> <b>Etymology:</b> —The specific epithet honors Gabriel Moreno, Spanish mycologist, for his extraordinary work of study of gasteroid fungi.</p> <p> <b>Type:</b> — RUSSIA. Republic of Tuva, Tandinsky district, near lake Kak-Khol, 51°21.415’ N, 94°23.497’ E, 701 m a.s.l, scattered shrub communities with <i>Caragana pygmaea</i>, on dry sandy soil, 8 July 2021, leg. <i>A</i> <i>.</i> <i>Vlasenko, NSK 1014816</i> (holotype), GenBank: OQ241379 (ITS), OQ236484 (28 S).</p> <p> <b>Other specimens examined:</b> — RUSSIA. Republic of Tuva, Tandinsky district, near lake Khadyn, 51°21.234’ N, 94°29.067’ E, 716 m a.s.l, sandy steppe, on dry sandy soil, 7 July 2021, leg. <i>A</i> <i>. Vlasenko, NSK 1014982</i>.</p> <p> <b>Ecology and distribution:</b> —Occurs in dry saline sandy habitats along lake shores. Grows singly, not in groups. Known only from Republic of Tuva.</p>Published as part of <i>Vlasenko, Vyacheslav A. & Vlasenko, Anastasia V., 2023, A new species of Tulostoma genus from Siberia, pp. 25-34 in Phytotaxa 600 (1)</i> on pages 28-30, DOI: 10.11646/phytotaxa.600.1.4, <a href="http://zenodo.org/record/8054168">http://zenodo.org/record/8054168</a>
Hohenbuehelia komarnitzkyi V. Vlasenko 2022, comb. nov.
Hohenbuehelia komarnitzkyi (Vassilkov) V. Vlasenko, comb. nov. Fig. 1 IndexFungorum: IF559494. Basionym:— Pleurotus komarnitzkyi Vassilkov (1961: 31). Type:— Turkmenistan, Kara-Kalinsky district, Kopetdag Mts., a valley of the Chandir, desert steppe, on the base of a stem of Eremostachys sp., 05 April 1959, S. A. Annaliev (holotype LE17692, isotype LE 5606). Additional specimens examined:— LE 5608, LE 5609. Distribution:—Known only from Turkmenistan and Tajikistan.Published as part of Vlasenko, Vyacheslav A., Turmunkh, Dejidmaa & Vlasenko, Anastasia V., 2022, New combination in the genus Hohenbuehelia (Pleurotaceae), pp. 200-204 in Phytotaxa 555 (2) on pages 203-204, DOI: 10.11646/phytotaxa.555.2.9, http://zenodo.org/record/687610
Diacheopsis cinerea A. Vlasenko & V. Vlasenko 2022, sp. nov.
Diacheopsis cinerea A. Vlasenko & V. Vlasenko, sp. nov. (Figs 1A–E, 2A–J) Index Fungorum number: IF557664; Facesoffungi number: FoF 08147 Sporocarps sessile, cylindrical, about 0.7–0.9 mm in diam. and 0.9–1.1 mm in total height, gregarious, scattered or in small groups of 2–8, drab grey, beige, densely clustered. Peridium single, membranous, translucent below, more dense at the top, fugacious above, persisting as a deep cup at the base; transparent, very light, with a slight greyish colour (in transmitted light) in the lower and lateral parts of the sporocarps; in the apex of sporotheca it is light-ocher, transparent (in transmitted light); it is often common in adjacent sporocarps, but with preservation of the side walls between adjacent sporocarps. Dehiscence is apical irregular. After the complete destruction of sporocarps, basal rings remain attached to the substrate. Columella absent. Capillitium dark violet-brown, with expanded nodes of dark brown colour, branched and anastomosed, arising from the base and forming a network connected to the peridium and the base of sporotheca by paler tips. Hypothallus continuous under the group (confluent), thin, light-coloured. Spore-mass dark brown to dark. Spores globose, 10.5–13.0 µm diam. including ornamentation, densely and uniformly covered by warts up to 0.4 µm in total height. In SEM, the ornamentation appears as dense, abundant and regularly distributed, well-developed, coralloid projections on the apex of each wart. Plasmodium not seen. Etymology:— Referring to the colour of sporocarps. Type:— RUSSIA. Altai Territory: near Losikha railway station, dry pine forest, on a fallen tree of Pinus sylvestris, 8 July 2009, leg. A. Vlasenko and V. Vlasenko, NSK 1030140 (holotype), GenBank SSU: MT 386336. Additional specimen examined:— RUSSIA. Altai Territory: near Losikha railway station, pine forest, on dead wood of Pinus sylvestris, 26 August 2019, leg. A. Vlasenko and V. Vlasenko, NSK 1030138 (paratype). Ecology:— Xylobiont. Distribution:— Known only from type locality. Comments:— Phylogenetically, Diacheopsis cinerea grouped with Lamproderma echinosporum and L. gulielmae. We have received data that other species of Diacheopsis are phylogenetically similar to those of the genus Lamproderma (Fig. 3). Diacheopsis metallica is closest to L. cristatum, while D. pauxilla is closest to L. sauteri and L. ovoideoechinulatum. Similar to Lamproderma, the morphological genus Diacheopsis consists of several separate phylogenetic lines. At the same time, the species that represent different phylogenetic lines demonstrate morphological similarities. Classification of the family Stemonitidaceae is not developed and is based on morphological features. Based on a complex of morphological features, we assigned the new species to the genus Diacheopsis. Diacheopsis cinerea has a warty ornamentation of spores, which brings it close to the group of species that have spores with warts: D. laxifila, D. minuta, D. mitchellii, D. pieninica, D. rigidifila, D. vermicularis, D. griseobrunnea and D. serpula. Spores of D. mithellii are very large, 20–21 µm in diam., covered with long spines or warted, about 1 mm in length and with tips that appear frayed under SEM. In comparison, the spores of D. cinerea are significantly smaller, 10.5–13.0 µm in diam., densely and uniformly covered with warts that are up to 0.4 µm in total height. By SEM, their ornamentation consists of dense, abundant, regularly distributed and well-developed coralloid projections on the apex of each wart. Spores of D. pieninica, D. rigidifila, and D. griseobrunnea are less than 9 µm in diam. (spores of D. cinerea are larger than 10 µm in diam.). Spores of D. laxifila are united into clusters while spores of D. cinerea are free. Sporocarps of D. minuta are very small, 0.2–0.4 mm in total height, of bronze colour while sporocarps of D. cinerea are 2–3 mm in total height, and grey-beige in colour. In D. serpula, elongated plasmodiocarps are predominant in the colony but in D. cinerea, sessile sporangia are predominant. Capillitium of D. serpula is without extensions while that of D. cinerea is with numerous filmy brown extensions. Capillitial nodes of D. vermicularis are rounded but in D. cinerea they are triangular and quadrilateral. The capillitial threads of D. vermicularis are colorless or grey and in D. cinerea they are dark violet-brown, and only the ends of threads by which they are attached to the base of sporocarp and peridium are light. The spores of D. vermicularis are light brown, with dark areas consisting of groups of warts (in transmitted light); those of D. cinerea are brown, and evenly ornamented with warts. In shape and size of sporocarps, the new species resembles D. insessa and D. nannengae, but it differs significantly by the structure of capillitium, size of the spores and their ornamentation. Spores of D. insessa are 16–22 µm in diam. and ornamented with long spines while the spores of D. cinerea are less than 14 µm in diam., and ornamented by warts. Spores of D. nannengae are ornamented with short spines while spores of D. cinerea have long warts up to 0.4 µm in total height. The capillitium of D. nannengae arises only from the base of sporotheca but in D. cinerea it arises from the base and forms a network connected by paler tips to the peridium and the base of sporotheca. Samples of D. cinerea were first found by us in 2009 in dry pine forests in the northern part of the “Verkhneobsky pine forest”, located on the right bank of Priobsky Plateau. Diacheopsis cinerea was collected there again 10 years later, which indicates the existence of a stable community of the species. The fructification of the species was observed after prolonged rains.Published as part of Vlasenko, Anastasia V., Vlasenko, Vyacheslav A. & Kabilov, Marsel R., 2022, A new species of Diacheopsis from Russia, pp. 193-200 in Phytotaxa 541 (2) on pages 195-199, DOI: 10.11646/phytotaxa.541.2.9, http://zenodo.org/record/638879
What are the implications of Curriculum Learning strategy on IRL methods?: Investigating Inverse Reinforcement Learning from Human Behavior
Inverse Reinforcement Learning (IRL) is a subfield of Reinforcement Learning (RL) that focuses on recovering the reward function using expert demonstrations. In the field of IRL, Adversarial IRL (AIRL) is a promising algorithm that is postulated to recover non-linear rewards in environments with unknown dynamics. This study investigates the potential benefits of applying the Curriculum Learning (CL) strategy to the AIRL algorithm. For our experiments, we use a randomized partially observable Markov decision process in the form of a grid-world-like environment. Using only expert demonstrations obtained with an RL algorithm under the true reward function, we train AIRL in a variety of configurations and identify an effective curriculum. Our results show, that a well-constructed curriculum can enhance the performance of AIRL twofold in both key aspects: the speed of convergence and the efficiency of using expert demonstrations. We thus conclude that CL can be a useful addition to an AIRL-based solution. Full code is available online in the supplementary material https://github.com/mikhail-vlasenko/curriculum-learning-IRL.CSE3000 Research ProjectComputer Science and Engineerin
Stemonitis amphorocolumella A. Vlasenko, G. Moreno & V. Vlasenko 2023, sp. nov.
Stemonitis amphorocolumella A. Vlasenko, G. Moreno & V. Vlasenko sp. nov. Figs. 1, 2. Mycobank: MB 829603 Description and Diagnosis: —Sporocarps in groups, dark brown, cylindrical, with total height of 6 – 9 mm. Hypothallus is dark brown, shiny, continuous under the colony. Sporotheca is long-cylindrical. The stalk is short, 25 – 40% of the total height, hollow, shiny, dark brown, almost black, reddish brown, in transmitted light orange. Peridium is not completely destroyed, remains in the form of a membranous collar at the base of sporotheca. Columella is cylindrical, similar in color to the stalk, with a bulge of amphora-like or bottle-like shape on top ends, up to 60 µm in diameter in the widest part. Capillitium is well developed, filaments extending from the column, are winding, dark brown, shiny and form a large-mesh network of different diameters (25–200 µm), well noticeable thickenings and/or membranous extensions are found in places of branching. The surface net consists of lighter, sometimes almost hyaline, threads, forming small meshes of 5 – 50 µm in diameter, with separate free endings in the form of spinules up to 12 µm in length. Spores are free, brown in mass, grayish brown in transmitted light, globose, with reticulate ornamentation, 9 – 11 µm in diameter, with 11 – 14 small meshes of reticulum on the visible side of the spore. Etymology: —Has a columella with an amphora-like extension. Type: — RUSSIA. Altai Territory, Aleysky district, 5 kilomerts northeast of the village Borovskoye, Lake Bakhmatovskoye, birch forest with Populus tremula, Padus avium, Caragana arborescens, on dead wood of Betula pendula, 52.689067° N, 82.249050° E, 235 m, substrate samples collected 24 August 2018, V.A. Vlasenko (holotype NSK 1026087). Additional specimens examined: — RUSSIA. Altai Territory, Aleysky district, 5 kilomerts northeast of the village Borovskoye, Lake Bakhmatovskoye, dry birch forest, on stump of Betula pendula, 52.687383° N, 82.245250° E, 233 m, substrate samples collected 24 August 2018, V. A . Vlasenko (paratype NSK 1026086, isoparatype AH 49360). Ecology: —Dead wood of deciduous trees. Distribution: —Currently known only from locality of this types: North Asia, southeast of Western Siberia. GenBank accession numbers: —SSU: OP831185. Comments: —The new species belongs to Stemonitis and differs from the Comatricha Preuss and Stemonaria Nann. -Bremek., R. Sharma & Y. Yamam. has in the double net of capillitium, forming a very lax internal net and a delicate complete surface net as well as a hollow, horny or fibrous stalk, consisting of faint closely set, longitudinal fibres. Among representatives of the Stemonitis genus, only four species have bulbous or membranous apex of the columella— Stemonitis capillitionodosa G. Moreno, D.W. Mitch., C. Rojas & S.L. Stephenson (Moreno et al. 2010), Stemonitis flavogenita E. Jahn (Janh 1904), Stemonitis pseudoflavogenita A. Vlasenko & Novozh. (Vlasenko et al. 2020) and Stemonitis sichuanensis B. Zhang & Yu Li (Zhang & Li 2016). Stemonitis amphorocolumella differs from similar species by several morphological features (Tab. 1). In these species, spores have ornamentation, consisting of spines and warts, while Stemonitis amphorocolumella has reticulate ornamented spores. The peridium of Stemonitis amphorocolumella is partially preserved in the form of a filmy collar at the base of sporotheca, whereas the peridium of S. capillitionodosa, S. flavogenita, S. pseudoflavogenita and S. sichuanensis is always fugacious. Stemonitis amphorocolumella differs from S. capillitionodosa by a well-developed small-mesh delicate complete surface net of capillitium, as well as thickenings in the places of branching of capillary filaments, but not membranous extensions. Stemonitis amphorocolumella differs from S. flavogenit a by the structure and form of the end of columella. The columella of Stemonitis flavogenit a ends with a large filmy extension, while the columella of S. amphorocolumella has a thickened non-filmy ending in the shape of an amphora or bottle. Stemonitis amphorocolumella differs from S. sichuanensis by significantly larger spores, up to 11 μm in diameter, and the presence of spinous free endings on filaments of the capillitium. Stemonitis amphorocolumella differs from S. pseudoflavogenita first of all spores with reticulate ornamentation and also structure and form of the end of columella. Columella of Stemonitis pseudoflavogenita always ending in a funnel-shaped expansion at the apex of the sporotheca in Table 2. The ML analysis based on the 18S nrDNA region showed that the new species is closest to Stemonitis fusca. The genetic distance of the “ Stemonitis amphorocolumella ” branch on the SSU tree is 0.166, with 94% bootstrap support (Fig. 3). The genetic distance of the “ Stemonitis fusca ” branch on the SSU tree is 0.276. Comparison of the sequences of Stemonitis amphoracolumella (OP 831185) and S. fusca (KP323386) aligned using the MAFFT for a common fragment of 797 base pairs showed their significant difference, including 183 nucleotide substitutions and 10 deletions and/or insertions). Thus, the conducted comparative morphological and phylogenetic analysis proves the species independence of Stemonitis amphorocolumella.Published as part of Vlasenko, Anastasia V., Moreno, Gabriel H. & Vlasenko, Vyacheslav A., 2023, Stemonitis amphorocolumella (Stemonitidaceae, Myxomycetes), a new species from Western Siberia, pp. 59-67 in Phytotaxa 592 (1) on pages 61-65, DOI: 10.11646/phytotaxa.592.1.5, http://zenodo.org/record/783567
Stemonitis Vlasenko & Moreno & Vlasenko 2023
Key to the species of the genus <i>Stemonitis</i> <p>1. Columella ending with expansions....................................................................................................................................................2</p> <p>- Columella ending without expansions...............................................................................................................................................6</p> <p> 2. Spores reticulate............................................................................................................................................... <i>S. amphorocolumella</i></p> <p>- Spores not reticulate..........................................................................................................................................................................3</p> <p> 3. Columella ending with membranous cup-like expansions........................................................................................... <i>S. flavogenita</i></p> <p>- Columella ending with widened expansion at the apex....................................................................................................................4</p> <p> 4. Capillitium without free ends.................................................................................................................................... <i>S</i>. <i>sichuanensis</i></p> <p>- Capillitium with free ends.................................................................................................................................................................5</p> <p> 5. Spores 9–11 µm diam., spinulose......................................................................................................................... <i>S</i>. <i>capillitionodosa</i></p> <p> - Spores, 7–8 µm diam., large warts and small warts......................................................................................... <i>S</i>. <i>pseudoflavogenita</i></p> <p>6. Spores reticulate................................................................................................................................................................................7</p> <p>- Spores not reticulate........................................................................................................................................................................11</p> <p>7. Reticulum with large or medium meshes on spores..........................................................................................................................8</p> <p>- Reticulum with small meshes on spores............................................................................................................................................9</p> <p> 8. Reticulum black, with 2–4 meshes across the spore. Spores dark brown in mass, red-brown in transmitted light, 8–9 µm diam......................................................................................................................................................................................... <i>S</i>. <i>inconspicua</i></p> <p> - Reticulum with 6 meshes across the spore. Spores bright lilac-brown in mass, pale lilac-brown, light reddish or pinkish brown in transmitted light, 6–8 µm diam................................................................................................................................... <i>S</i>. <i>virginiensis</i></p> <p> 9. Reticulum with 8–11 meshes across the spore. Spores reddish-brown to dark brown in mass, brownish-grey in transmitted light, 8–9 µm diam..................................................................................................................................................................... <i>S</i>. <i>marjana</i></p> <p>- Reticulum with 6–9 meshes across the spore..................................................................................................................................10</p> <p> 10. Spores gray-purple, brown-purple in mass, purple-brown in transmitted light, 7–9 µm diam., sporocarps 6–20 mm total height................................................................................................................................................................................................... <i>S</i>. <i>fusca</i></p> <p> - Spores dark brown in mass, purple gray in transmitted light, 8–9 µm diam., sporocarps 3 mm total height.................. <i>S</i>. <i>foliicola</i></p> <p> 11. Spores in clusters................................................................................................................................................................ <i>S</i>. <i>uvifera</i></p> <p>- Spores free.......................................................................................................................................................................................12</p> <p>12. Sporocarps large or medium 7–20 µm............................................................................................................................................13</p> <p>- Sporocarps small up to 5 (7) µm.....................................................................................................................................................16</p> <p> 13. Sporocarps light brown, spores 5.0–7.5 µm diam., with very fine pale warts seen by oil-immersion.............................. <i>S</i>. <i>axifera</i></p> <p>- Sporocarps dark brown to black......................................................................................................................................................14</p> <p> 14. Capillitium without internal net, surface net with large meshes 60–275 µm diam.................................................... <i>S</i>. <i>rhizoideipes</i></p> <p>- Capillitium with internal and surface net........................................................................................................................................15</p> <p> 15. Surface net irregular, many expansions in the axils of the surface net capillitium............................................................... <i>S</i>. <i>plana</i></p> <p> - Surface net without many expansions in the axils of the surface net capillitium........................................................... <i>S</i>. <i>splendens</i></p> <p>16. Sporocarps light brown....................................................................................................................................................................17</p> <p>- Sporocarps dark brown to black......................................................................................................................................................19</p> <p> 17. Stalk long, 1/3–1/2 of the total height................................................................................................................................ <i>S</i>. <i>pallida</i></p> <p>- Stalk short, 1/5 of the total height....................................................................................................................................................18</p> <p> 18. Sporocarps gregariously tufted on a common hypothallus. Hypothallus a rather thick, dark red brown membrane, darker at the stalk bases.......................................................................................................................................................................... <i>S. graciliformis</i></p> <p> - Sporocarps in groups. Hypothallus membranous, inconspicuous.................................................................................. <i>S</i>. <i>herbatica</i> 19. Spores small, up to 7 µm diam......................................................................................................................................... <i>S</i>. <i>farrensis</i> - Spores large 8.5–12.5 µm diam.......................................................................................................................................................20</p> <p> 20. Spore-mass black. Spores with long warts, up to 0.8 µm....................................................................................... <i>S</i>. <i>mussooriensis</i> - Spore-mass pale brown. Spores with short warts............................................................................................................................21</p> <p> 21. Capillitium smooth, not bearing spines internal. Internal net with 3–4 meshes across the radius..................... <i>S. mediterraneensis</i></p> <p> - Capillitium lax, with short thick, protrusions on the outside, rather wide, flat to rounded, all the axils rounded and with membranous expansions. Internal net with 1–2 meshes across the radius............................................................................................... <i>S</i>. <i>laxifila</i></p>Published as part of <i>Vlasenko, Anastasia V., Moreno, Gabriel H. & Vlasenko, Vyacheslav A., 2023, Stemonitis amphorocolumella (Stemonitidaceae, Myxomycetes), a new species from Western Siberia, pp. 59-67 in Phytotaxa 592 (1)</i> on pages 64-65, DOI: 10.11646/phytotaxa.592.1.5, <a href="http://zenodo.org/record/7835677">http://zenodo.org/record/7835677</a>
Leccinum anastasiae V. Vlasenko 2023, sp. nov.
<i>Leccinum anastasiae</i> V. Vlasenko, <i>sp. nov.</i> Fig. 1. <p>MycoBank: MB 844740.</p> <p> <b>Type:</b> — RUSSIA. The Republic of Altai, near Dzhazator (Belyashi) village, a habitat with solitary <i>Larix</i> Mill. and <i>Salix</i> L. trees on the border with a steppe meadow and a bog in the river’s oxbow, on soil under <i>Salix bebbiana</i> Sarg., 49.6914° N, 87.4344° E, 1569 m., 21 August 2019, <i>V.A. Vlasenko</i> and <i>A.V. Vlasenko</i> (holotype NSK 1014999).</p> <p> <b>Etymology:</b> —Named after mycologist Anastasia Vlasenko.</p> <p> <b>Description:</b> —Basidiomata is medium-sized. Pileus is up to 4 cm in diam., convex, up to 1.4 cm thick, lacking a sterile margin; surface is dry, glabrous, rugulose, dark cream with a light marble pattern; context is thin, 1 mm thick, in the center of the pileus is up to 1 cm thick, white, and does not change color when bruised but may darken slightly; bright colors absent. Hymenophore is poroid; pores are angular, 0.3–0.6 mm in diam., concolorous, light cream, staining cinnamon brown if bruised and with age; tubes adnexed to deeply depressed around the stipe, up to 1.3 cm in length, tube walls are 0.05 mm thick, white-greyish-cream when young to pale cinnamon-brown with age. Stipe is 5 cm long, 0.6–1.0 cm broad, central, cylindrical, slightly curved, dense, fragile; surface dry, white, scabrous, scales are white at first, becoming pale to pale-cream, staining fuscous if bruised; context is white, light cream with age; basal mycelium is white. No distinctive odour. Basidiospores are 5.4–6.90 × 14.2–23.60 μm, Q min = 2.62, Q max = 3.42, Qm = 2.96, spindle-shaped to elongate-ellipsoid, creamy brown with greenish-brown protoplast in KOH, smooth. Basidia are 6.5–11 × 20–28.5 μm, clavate, thin-walled, 2–4-spored, hyaline in KOH; sterigmata are 4–5 μm in length. Hymenophoral trama is boletoid, hyphae are subcylindrical, 3–7 μm wide, and hyaline. Cheilo- and pleurocystidia are 7–12 × 18–30 μm, spindle-shaped to fusiform, thin-walled, hyaline. Pileipellis is a trichoderm composed of filamentous hyphae 3.5–10 μm wide, thin-walled, hyaline. Pileus trama is composed of hyphae 2.5–11 μm wide, thin-walled, hyaline. Stipitipellis is composed of hyphae 2–8 μm wide, thin-walled, hyaline. Caulocystidia are 9–10 × 18–28 μm, spindle-shaped to fusiform, thin-walled, hyaline. Stipe trama is composed of hyphae 3–10 μm wide, thin-walled, hyaline. Clamp connections are absent in all tissues.</p> <p> <b>Additional specimen examined:</b> — RUSSIA. The Republic of Altai, near Dzhazator (Belyashi) village, the right bank of the Argut River, swampy habitat with <i>Salix</i> L. trees by the river, on soil, 49.7244° N, 87.3941° E, 1601 m., 23 August 2019, <i>A. V</i> <i>.</i> <i>Vlasenko</i> (paratype NSK 1014981).</p> <p> <b>Habitat:</b> —Scattered on the ground under <i>Salix bebbiana</i> Sarg., near bogs in the river valleys between mountain ranges.</p> <p> <b>Distribution:</b> —Southeast Altai (Kosh-Agach district of the Republic of Altai).</p> <p> <b>GenBank accession numbers:</b> ITS: ON524838, LSU: ON514264; <i>EF-1α</i>: OR758905; <i>RPB2</i>: OR758904.</p> <p> <b>Comments:</b> —The new species <i>Leccinum anastasiae</i> does not have orange and red tints in the cap color. Based on this, it is easy to differentiate it from other species of the genus, which have brightly colored fruiting bodies. The cap surface of <i>Leccinum anastasiae</i> is creamy brown, and the surface of its pores becomes tobacco-like over time. It differs from the closely related species, <i>Leccinum schistophilum</i> and <i>L</i>. <i>snellii</i> A.H. Sm., Thiers et Watling (1967: 120), by the absence of pink coloration in the upper and blue-green coloration in the lower part of the stipe when bruised.</p> <p> The ITS1 nrDNA region in <i>Leccinum</i> species contains many tandem repeats (Den Bakker <i>et al.</i> 2004). The analysis of our aligned dataset showed the presence of 19 regions with “TATTGAAAA” repeats. The maximum number of such repeats in some sequences is up to 11 in <i>Leccinum holopus</i>. In addition, there were 15 regions with “CTAATAGAAA” repeats, with a maximum number of repeats up to eight, also in <i>Leccinum holopus</i>. The presence of such mini satellites negatively affects the phylogenetic reconstruction for the genus. Therefore, we performed a phylogenetic analysis of the ITS1-5.8S-ITS2 nrDNA region, excluding mini satellites from the sequences. Introns were also excluded from the sequences of all genes.</p> <p> The ML analysis based on the ITS1-5.8S-ITS2 nrDNA region showed that the new species is closest to <i>Leccinum schistophilum</i> (Fig. 2). The genetic distance of the “ <i>Leccinum anastasiae</i> ” branch on the ITS tree is 0.022, with 97% bootstrap support.</p> <p> In addition, the ML analysis based on the 28S+ <i>EF-1α</i> + <i>RPB2</i> DNA regions confirmed that the new species is closest to <i>Leccinum schistophilum</i> (Fig. 3). The genetic distance of the “ <i>Leccinum anastasiae</i> ” branch on the tree is 0.007, with 87% bootstrap support.</p> <p> Despite some inconsistency in the topology of our ITS1-5.8S-ITS2 and 28S+ <i>EF-1α</i> + <i>RPB2</i> trees, the new species of <i>Leccinum anastasiae</i> had the most similarity with <i>L</i>. <i>schistophilum</i>. Based on that, we performed a separate comparison of the sequences of these two species. It turned out studied sequences ITS1-5.8S-ITS2 and parts of the 28S, <i>EF-1α</i> and <i>RPB2</i> regions was quite variable, which allows us to declare the independence of these species.</p> <p> Substrate and geographic factors are also crucial for species diagnostics. <i>Leccinum holopus</i>, <i>L</i>. <i>schistophilum</i> and <i>L</i>. <i>snellii</i> growing on swamps form symbiotic associations with <i>Betula</i> L. (1753: 982) spp. The new species <i>Leccinum anastasiae</i> is associated with a unique symbiont, <i>Salix bebbiana</i>.</p>Published as part of <i>Vlasenko, Vyacheslav A., 2023, A new Leccinum species from the Altai Mountains, pp. 191-200 in Phytotaxa 625 (2)</i> on pages 194-197, DOI: 10.11646/phytotaxa.625.2.5, <a href="http://zenodo.org/record/10150942">http://zenodo.org/record/10150942</a>
Stemonitis amphorocolumella (Stemonitidaceae, Myxomycetes), a new species from Western Siberia
Vlasenko, Anastasia V., Moreno, Gabriel H., Vlasenko, Vyacheslav A. (2023): Stemonitis amphorocolumella (Stemonitidaceae, Myxomycetes), a new species from Western Siberia. Phytotaxa 592 (1): 59-67, DOI: 10.11646/phytotaxa.592.1.5, URL: http://dx.doi.org/10.1094/PDIS-04-22-0755-PD
A new species of Diacheopsis from Russia
Vlasenko, Anastasia V., Vlasenko, Vyacheslav A., Kabilov, Marsel R. (2022): A new species of Diacheopsis from Russia. Phytotaxa 541 (2): 193-200, DOI: 10.11646/phytotaxa.541.2.9, URL: http://dx.doi.org/10.11646/phytotaxa.541.2.
FIGURE 2 in A new species of Tulostoma genus from Siberia
FIGURE 2. Мicromorphological characters of Tulostoma morenoi (holotype). (a, c) Spores, capillitial threads (SEM). (b) Spores (SEM). (d) Spore warts with ridge-like anastomoses (SEM). (e) Spore, spore warts (SEM). Scale bars: a—30 μm, b, c, e—2 μm, d—1 μm.Published as part of Vlasenko, Vyacheslav A. & Vlasenko, Anastasia V., 2023, A new species of Tulostoma genus from Siberia, pp. 25-34 in Phytotaxa 600 (1) on page 29, DOI: 10.11646/phytotaxa.600.1.4, http://zenodo.org/record/805416
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