1,721,002 research outputs found
The effect of Laurus nobilis on the blood and lenses antioxidant activity in rabbit under fat-enriched diet
Full text linkFat-enriched diet is strongly associated with cataract development. Laurus nobilis shows antioxidant activity. Herein we evaluated the effect of Laurus nobilis oral administration on the blood and lenses antioxidant activity in rabbits under fat-enriched diet. Sixty rabbits divided into 4 groups were used. One group represented the control (N-CTR). The second group (P-CTR) fed a diet supplemented with 2.5 % of pig fat; the third group (EXP1) received a diet supplemented with 2.5 % of pig fat and 1 g/kg of dried-bay leaves; the fourth group (EXP2) was treated with dried-bay leaves at the rate of 1 g/kg of feed. At baseline and at the end of the study (56 days) the following blood parameters were determined: thiobarbituric acid reactive substances (TBARS), reactive oxygen metabolites (ROMs), total phenols, superoxide dismutase (SOD), oxygen radical absorbance capacity (ORACpca), ferric ion reducing antioxidant power (FRAP), retinol and alfa-tocopherol. At the end of the follow-up, the eyes were enucleated and the antioxidant profile, such as total antioxidant activity (TAC), TBARS, retinol and alfa-tocopherol of lenses was evaluated. Plasma ROMs and TBARS levels were statistically lower in the groups receiving bay leaves integration. A significant increase of plasma retinol, FRAP and ORACpca levels was found in EXP1 and EXP2 groups, whereas plasma alfa-tocopherol resulted statistically higher only in EXP2 group. Bay leaves supplementation enhanced TAC, retinol and alfa-tocopherol in rabbit lens, particularly in EXP2 group; whereas lenses TBARS levels significantly decreased in both treated groups. These findings demonstrate that Laurus nobilis oral administration exerts a protective effect on the risk of cataract development in rabbits under fat-enriched diet
Rabbit metabolic response and selected meat quality traits: Evaluation of dietary PLX®23 and LycoBeads® feed supplement.
No full textEffects of dietary supplementation of verbascoside and lycopene, alone and in combination, on some blood parameters, plasma oxidative status and meat quality traits in intensively reared rabbits were investigated. The test lasted 60 days and was conducted on 200 weaned-rabbits, divided into four groups of 50 animals each (5 animals each cage × 10 repetitions). The control group (CON) received a fattening feed without any feed supplements, whereas the experimental groups received the following: the first, an integration in 22 g of PLX ® 23 (Lippia citriodora extract; VB group) per ton of feed; the second, an integration in 100 g of LycoBeads ® (Solanum lycopersicum extract; LIC group) per ton of feed; and the third, an integration of both feed supplements in combination at the same doses (LIC+VB group). The dietary supplementation with Lippia citriodora and Solanum lycopersicum resulted in an improvement of the blood lipid profile, oxidative plasma markers, and hepatic and renal activity of treated growing rabbits. Feed additives also produced meat with a lower content in SFA and an increased PUFA content, and inhibited the lipid oxidation improving the oxidative stability of rabbit meat. The dietary supplementation, even reporting no effects on productive parameters and carcass yield, showed an improvement on several blood parameters, as indicator of animal welfare, and on quality and healthy meat markers. © 2018 Blackwell Verlag Gmb
Assessment of a natural dietary extract, titrated in phenylpropanoid glycosides, on blood parameters and plasma oxidative status in intensively reared Italian hares (Lepus corsicanus)
No full textTwo different doses of a natural extract titrated in phenylpropanoid glycosides (PPGs) were evaluated for their effect on blood parameters and plasma oxidative status in pairs of intensively reared Italian hares. The study lasted 210 days, during which 45 couples of hares were divided into three homogeneous groups. A control group (CON) was fed a control diet while the two experimental groups were fed a diet supplemented with 1 or 2 kg/t of a supplement titrated in PPGs. Blood samples were obtained at 0, 70, 140 and 210 days and assayed for plasma lipid profiles, bilirubin, haematological parameters and indicators of oxidative status (reactive oxygen metabolites (ROMs), thiobarbituric acid reactive substances (TBARS), vitamins A and E). Although dietary treatment did affect the levels of triglycerides, total cholesterol and total bilirubin, all of which decreased markedly (P < 0.05), while significantly increasing the (P < 0.01) HDL cholesterol values, it also significantly improved the oxidative status of the blood, which displayed an increase in both vitamin E (P < 0.01) and vitamin A (P < 0.05) and a decrease in ROMs (P < 0.01) and TBARS (P < 0.05). The improvements in the blood parameters, lipid profile and plasma oxidative status continued to increase significantly as the trial progressed, indicating a positive effect with increased length of treatment. The results of this study demonstrate an important role for feed supplementation with respect to antioxidant activity on some blood parameters, including the lipid profile and the oxidative status of blood
Effect of dietary extruded linseed, verbascoside and vitamin E supplements on selected serum biochemical parameters and plasma oxidative status in Lacaune ewes
No full textSelected serum biochemical parameters and plasma oxidative status were evaluated in Lacaune ewes on a diet supplemented with extruded linseed, verbascoside and vitamin E. A 98 day-trial was conducted on 44 ewes and started 40 ± 2 days post partum. The animals were divided into four homogeneous groups of eleven animals each; one control group (CON) with a basal diet, and the other three experimental groups supplemented with extruded linseed (L group), extruded linseedverbascoside (LVB group) and extruded linseed-verbascoside-vitamin E (LVBE group). All animals individually received an isoproteic and isoenergetic diet, consisting of 700g of concentrated feed and meadow hay ad libitum. Blood sampling of the ewes was performed three times: at the beginning (0 d), midway (49 d) and end of the trial (98 d). The following parameters were determined: triglycerides, total cholesterol, high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, bilirubin, alanine aminotransferase, aspartate aminotransferase, thiobarbituric acid reactive substances (TBARS), reactive oxygen metabolites (ROMs), vitamin A and vitamin E. Dietary integration in the LVB and LVBE groups resulted in an improvement in the blood profile, characterized by a significant (P=0.001) increase in HDL cholesterol and a marked decrease in triglycerides (P=0.001), total cholesterol (P=0.017), LDL cholesterol (P=0.001) and bilirubin (P=0.035), due to the verbascoside supplementation. In the same groups, there was an increase in blood vitamin A (P=0.002) and vitamin E (P=0.001), and a reduction (P=0.001) in ROMs and TBARS, with an improved plasma oxidative status. The dietary vitamin E integration did not produce a significant improvement in the parameters studied, with no statistical differences between LVB and LVBE groups. Animal feed containing extruded flaxseed, might therefore benefit from the addition of a verbascoside supplement, because of the positive effect showed by this molecule on the blood parameters
The effect of water-restriction on various physiological variables in intensively reared Lacaune ewes
Full text linkThe effects of water restriction on rectal temperature, respiratory rate and selected blood variables in intensively reared Lacaune ewes were evaluated. The tests were carried out over the course of 28 days in spring on 30 lactating sheep divided into three groups of 10 animals each, matched by lactation status and body weight and allocated into individual boxes. The animal groups were as follows: control group W100 with drinking water for the whole day (24 h/24 h), while the two experimental groups each received 80% (W80 group) and 60% (W60 group) of the water ration of the control group, respectively. The feed, in according with physiological and production needs of the animals, was administered in three daily meals (08:00, 14:00 and 20:00 h). Water intake was recorded three times per day (at 08:00, 14:00 and 20:00 h) and the daily feed intake at 08:00 h, while the rectal temperature, respiratory rate were measured and blood samplings were carried out on Day 0, Day 14 and Day 28. In water-restriction groups (W80 and W60) a lower (P < 0.01) feed intake of meadow hay, and a lower ingestion of alfalfa hay pellet in the W60 group compared to W100 group were observed. In addition, the water restriction regimen resulted in a significant decrease of respiratory rate (P < 0.05) and an increase in serum total protein, urea, creatinine, sodium, chlorine, reactive oxygen metabolites, cortisol, haemoglobin and mean corpuscular haemoglobin concentration (P < 0.05), red blood cell counts, and haematocrit (P < 0.01). These results show the important role of water, as limiting factor for animal breeding in low-water availability environments. Where possible, the management of low-dairy breeds should be well adapted to support the productive performance
Fatty acids profile in Carassius spp. From Lake Gopło, Poland
No full textEffects of sex and age on fatty acids profile in the meat of Carassius spp. were evaluated. Carassius auratus fillets displayed a higher content of SFA and MUFA than Carassius carassius. In contrast, Carassius carassius had a higher content of PUFA (higher proportion of linoleic, arachidonic, eicosapentaenoic, docosatetraenoic and docosapentaenoic acids) and total n-3 and n-6 PUFA, compared with Carassius auratus. Moreover, γ-linolenic acid was higher in fillets from Carassius auratus. What is more, fillets from females had a greater content of eicosapentaenoic acid than males. Finally, 4-year-old fish had a lower content of ALA, higher MUFA content and better nutritional indexes (n-3/n-6, AI) than 3-year-old fish
Effect of dietary phenylpropanoid glycoside-based natural extracts on blood parameters and productive performance in intensively-reared young hares.
No full textEffects of verbascoside on plasma oxidative status and blood and milk production parameters during the peripartum period in Lacaune ewes
No full textThis study examined the effect of administration of verbascoside on plasma oxidative status and on specific blood and milk production parameters in Lacaune ewes during the peripartum period. The trial, lasting 60. d, ran from 20. d before lambing until 40. d after and was conducted on 40 ewes in their 5th month of gestation. The animals were divided into two equal groups of 20: a control group CON, which did not receive food supplement, and the experimental group V, which received a supplement of verbascoside in their feed. Consumption of the supplement, which was 2. g/ewe/d, was assured by individual and daily administration of feed. The animals received a pellet feed concentrate at a rate of 400. g/ewe/d prepartum and 700. g/ewe/d postpartum. The ration was completed with mixed hay ad libitum. The experimental measures included body weight; blood samples 20. d before lambing and 0. d, 20. d and 40. d after lambing; body condition score; individual yield and quality of milk. The administration of verbascoside significantly improved milk yield by 9.3% (P< 0.01), significantly decreased triglycerides, total cholesterol, low-density lipoprotein cholesterol, alanine transferase (P< 0.01) and bilirubin (P< 0.05), and increased high density lipoprotein cholesterol (P< 0.01). Furthermore, the parameters of oxidative status in plasma were positively influenced by the use of dietary verbascoside, which resulted in significant decreases in reactive oxygen metabolites (P< 0.01) and thiobarbituric acid-reactive substances (P< 0.05) and increases in vitamins A and E (P< 0.01).The use of verbascoside during the peripartum period provided benefits on some blood parameters, oxidative status and milk production, particularly in the immediate postpartum period (0-20. d). © 2011 Elsevier B.V
Effect of dietary &lt;i&gt;Lippia citriodora&lt;/i&gt; extract on productive performance and meat quality parameters in hares (&lt;i&gt;Lepus europaeus Pall.&lt;/i&gt;)
No full textAbstract. Three different dietary doses of Lippia citriodora natural extract were tested in order to verify their effect on productive performance and the meat quality of intensively-reared hare (Lepus europaeus Pall.). The 240 day-trial was conducted on 20 male hares, divided into 4 homogeneous groups of 5 animals each. A control group received a basal diet without any supplementation, and the other three experimental groups received a natural extract of Lippia citriodora, titrated in verbascoside, in the following amounts: 1 g, 1.5 g and 2 g of natural extract/kg feed in the low (LNE), medium (MNE), and high natural extract (HNE) groups, respectively. Natural extract enhanced the quality of the meat: there was a significant decrease (P<0.05) in saturated fatty acids and a significant increase (P<0.05) in mono- and poly-unsaturated fatty acids. The oxidative stability of the meat improved, thus highlighting a possible link between the decrease (P<0.05) in TBARS values and an increase (P<0.05) in lipid vitamin content. The cholesterol content of the meat decreased markedly (P<0.01) after the dietary verbascoside treatment, thus improving the health benefits of the meat. These results clearly show the important role of Lippia citriodora extract, titrated in verbascoside, in improving the quality of the meat of intensively-reared hares. In addition the present paper underlines how the use of natural antioxidant in the animal feeding system may provide healthier and low-oxidized products to the final consumer.
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Ocular tissues and fluids oxidative stress in hares fed on verbascoside supplement
No full textThe influence of a prolonged diet supplemented with the powerful antioxidant verbascoside on the oxidative state of 20 healthy hares eye fluids and tissues has been studied. Verbascoside was dosed at 2, 3, 4 mg/die and the impact on the oxidative state of ocular tissues and fluids was tested by TBARS (thio barbituric acid reactive substances) and TEAC (trolox equivalent antioxidant capacity) assays. The percentage of change in antioxidant activity increased largely in retina and lenses at a daily verbascoside dose of 3 mg, whereas for optic nerve and vitreous humor the higher antioxidant capacity was measured at 4 mg/die verbascoside dose. The present findings demonstrate that verbascoside supplementation is able to protect ocular tissue and fluids from naturally occurring oxidation and that its protective effect depends on the daily dose, being maximum up to 3 mg/die
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