754 research outputs found
Editorial: Recent Advances in Recombinant Antibody Therapeutics and Diagnostics for Infectious Diseases
No full textAntibodies to the FbsA protein of streptococcus agalactiae and their use in treating or preventing infections
No full textMonoclonal and polyclonal antibodies are provided which can bind to the FbsA protein of Streptococcus agalactiae (GBS) and which can be used to prevent adherence of the bacteria to host cells and thus be useful in the treatment and protection against infection from S. agalactiae. The antibodies of the invention can also be raised against the fibrinogen binding domain of FbsA or the repeat region therein, and in addition to preventing bacterial adherence, the antibodies to FbsA are advantageous in that they can be used to prevent platelet aggregation and thrombus formatio
Falsely Elevated Whole Blood Cyclosporine Concentrations Measured by an Immunoassay With Automated Pretreatment
No full textTherapeutic drug monitoring of
cyclosporine A (CyA) blood concentrations
is needed to ensure therapeutic
efficacy and to prevent toxicity, because
it exhibits marked pharmacokinetic variability
and, in the case of high concentrations,
a dose reduction is required
Gold Nanoparticles Contact with Cancer Cell: A Brief Update
No full textThe fine-tuning of the physicochemical properties of gold nanoparticles has facilitated the rapid development of multifunctional gold-based nanomaterials with diagnostic, therapeutic, and therapeutic applications. Work on gold nanoparticles is increasingly focusing on their cancer application. This review provides a summary of the main biological effects exerted by gold nanoparticles on cancer cells and highlights some critical factors involved in the interaction process (protein corona, tumor microenvironment, surface functionalization). The review also contains a brief discussion of the application of gold nanoparticles in target discovery
Editorial: Cells, Biomaterials, and Biophysical Stimuli for Bone, Cartilage, and Muscle Regeneration
No full textVisai, Livia/0000-0003-1181-3632; Fassina, Lorenzo/0000-0002-5587-4632; Ramalingam, Murugan/0000-0001-6498-9792; Cusella De Angelis, Maria Gabriella/0000-0003-2642-3346[No Abstract Available
METHOD FOR MAKING ANTIBACTERIAL AND ANTIVIRAL THE SURFACES OF METAL PRODUCTS INTENDED FOR MEDICAL USES
No full textThe method for making antibacterial and/or antiviral the surfaces of metal products intended for medical uses comprises:
cleaning said surfaces; making said surfaces attached to inorganic agents; applying on said surfaces an inorganic barrier having
antibacterial and/or antiviral characteristics, in such a way that said inorganic barrier is placed in between said surfaces and bacteria and viruses; making said application stable
L’importanza della nanotopografia per le interazioni in vitro cellula-superficie
No full textLo studio delle interazioni cellula-superficie in vitro è di fondamentale importanza
per gli aspetti applicativi di un biomateriale. L’adesione cellulare (eucarioti/
procarioti), la migrazione, la proliferazione e la differenziazione sono
processi biologici che sono influenzati sia dalla composizione chimica che dalla
topografia della superficie del biomateriale impiegato. In ambito medico, l’applicazione
progressiva di superfici micro-nanostrutturate ha acquisito crescente interesse
al fine di poter migliorare la citocompatibilità e l’integrazione tissutale. È
quindi di grande interesse sia la comprensione delle interazioni cellula-superficie
sia l’utilizzo di nuove tecniche biofisiche che consentano di determinare quantitativamente
le interazioni a livello dei singoli componenti molecolari coinvolti
nel processo di interazione. Per questo motivo i fattori fisici sono importanti, al
pari di quelli biochimici, per determinare la risposta cellulare ad una specifica
superficie.
In questo capitolo, cercheremo di: I) definire i meccanismi principali delle interazioni
cellula-superficie in vitro, soprattutto a livello di nanoscala per effetto
di nanoprotrusioni, nanocavità e nanoscanalature; II) illustrare le recenti tecniche
biofisiche che hanno aperto nuove prospettive nell’ambito delle funzioni biologiche
e introdotto un nuovo elemento di determinazione quantitativa in questo
campo
Can Nanotechnology Shine a New Light on Antimicrobial Photodynamic Therapies?
No full textPhotomedicine is one of the most inspiring and interdisciplinary fields in medicine that involves the research and application of photobiology with respect to health and disease. Photomedicine has contributed to the clinical practice of a variety of medical fields, including dermatology, surgery, radiology, diagnostics, cardiology, and anticancer therapy. Furthermore, expansion of its scope and contribution can be expected. This book covers a wide range of aspects and issues related to photomedicine, which brings together researchers from many countries. These include the basic science of photodynamic therapy, clinical applications in various kinds of medical fields, photochemotherapy, laser therapy for musculoskeletal pain, intense pulsed light therapy for photorejuvenation, biological function of low-level laser therapy, and photobiology for skin rejuvenation. Not only will this be beneficial for readers, but it will also contribute to scientists making further breakthroughs in photomedicine
Isolation and characterization of a novel collagen-binding protein from Streptococcus pyogenes strain 6414
No full textIn this report we have analyzed the binding of collagen to Streptococcus pyogenes strain 6414. This binding was rapid, specific, and involved a limited number of receptor molecules (11,600 copies per cell). When the proteins in a streptococcal lysate were blotted onto a nitrocellulose filter and probed with 125I-labeled collagen, a prominent collagen-binding protein of 57 kDa was identified as well as minor 130-150-kDa components. The major 57-kDa protein was isolated by affinity chromatography on collagen-Sepharose followed by gel filtration chromatography. The 57-kDa protein purified from S. pyogenes was used to raise a monospecific antibody which also reacted with a collagen-binding protein of similar molecular size isolated from Streptococcus zooepidemicus. The two collagen-binding proteins from streptococci have a similar amino acid composition and isoelectric points. Isolated collagen-binding protein was specifically recognized by 125I-collagen in a solid-phase binding assay and displayed an affinity for the ligand quite similar to that exhibited by intact bacteria (Kd = 3.1 versus 3.5 x 10(-9) M, respectively). Surface-labeled bacteria attached to microtiter wells coated with different collagen types and the 57-kDa protein blocked the adhesion to collagen substrate. We propose that the 57-kDa protein is an adhesin involved in the attachment of streptococci to host tissues
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