30 research outputs found
New Insight into Biodegradation of Poly (L-Lactide), Enzyme Production and Characterization
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Purification and characterization of extracellular .BETA.-xylosidase and .BETA.-glucosidase from Aspergillus fumigatus.
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Purification and characterization of a high-thermostable β-xylanase from newly isolated Thermomyces lanuginosus THKU-49
No full textSelection of High β β β β β-Glucanase Produced Aspergillus Strain and Factors Affecting the Enzyme Production in Solid State Fermentation
No full textABSTRACT Among five β-glucanase producing strains, Aspergillus terreus ASKU 10 was selected as the potential strain for the enzyme production in solid state fermentation using rice straw as substrate. Six factors, addition of rice bran, spore inoculums, cultivation time, initial moisture content, corn steep liquor (CSL) and KH 2 PO 4 contents were optimized using "one-factor-at-a-time" method for β-glucanase production. The highest β-glucanase activity achieved under the condition as following: 10 g rice straw without addition of rice bran, with 10 7 spore inoculums, 5 days of cultivation time, 75% initial moisture content, 3.3 ml CSL and 0.825 g KH 2 PO 4 . Under this optimal condition β-glucanase production with 4,054 U/g dry substrate was obtained
Poly(dl-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes
No full textPurification and Characterization of a Thermostable Xylanase from<i>Saccharopolyspora pathumthaniensis</i>S582 Isolated from the Gut of a Termite
No full textPoly(dl-lactide)-degrading enzyme production by immobilized Actinomadura keratinilytica strain T16-1 in a 5-L fermenter under various fermentation processes
No full textBackground: Poly(dl-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading enzyme production by bacteria. Therefore, the aims of this study were to determine a suitable immobilization material for PDLLA-degrading enzyme production and optimize PDLLA-degrading enzyme production by using immobilized A. keratinilytica strain T16-1 under various fermentation process conditions in a stirrer fermenter. Results: Among the tested immobilization materials, a scrub pad was the best immobilizer, giving an enzyme activity of 30.03 U/mL in a shake-flask scale. The maximum enzyme activity was obtained at aeration 0.25 vvm, agitation 170 rpm, 45°C, and 48 h of cultivation time. Under these conditions, a PDLLA-degrading enzyme production of 766.33 U/mL with 15.97 U/mL·h productivity was observed using batch fermentation in a 5-L stirrer fermenter. Increased enzyme activity and productivity were observed in repeated-batch (942.67 U/mL and 19.64 U/mL·h) and continuous fermentation (796.43 U/mL and 16.58 U/mL·h) at a dilution rate of 0.013/h. Scaled-up production of the enzyme in a 10-L stirrer bioreactor using the optimized conditions showed a maximum enzyme activity of 578.67 U/mL and a productivity of 12.06 U/mL·h. Conclusions: This research successfully scaled-up the enzyme production to 5 and 10 L in a stirrer fermenter and is helpful for many applications of poly(lactic acid). Keywords: Actinomycete, Biodegradable polymer, Bioreactor, Cell immobilization, Depolymerase, Fermentation, Immobilization, Plastic wastes, Poly(dl-lactic acid
