1,721,051 research outputs found
Characterization of mechanical properties of ECM nanostructures of tendons and ligaments
No full textREACHING THE NORMAL DIFFUSION REGIME WITH AN ATOMIC INFORMED COARSE GRAIN MODEL: DIFFUSION OF BENZENE WITHIN PVA MATRIX
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Biological performances of collagen-based scaffolds for vascular tissue engineering
No full textCollagen is widely used for biomedical applications and it could represent a valid alternative scaffold material for vascular
tissue engineering.In this work, reconstituted collagen films were prepared from neutralized acid-soluble solutions for subsequent
haemocompatibility and cell viability performance assays.First, haemoglobin-free, thrombelastography and platelet adhesion tests
were performed in order to investigate the blood contact performance.Secondly, specimens were seeded with endothelial cells and
smooth muscle cells, and cell viability tests were carried out by MTT and SEM.Results show that neutralized acid-soluble type I
collagen films do not enhance blood coagulation, do not alter normal viscoelastic properties of blood and slightly activate platelet adhesion and aggregation.Cell culture shows that the samples are adequate substrates to support the adhesion and proliferation of endothelial and smooth muscle cells
Molecular Analysis Of Interaction Energies Of The Decorin Proteoglycan-Collagen Complex In Tendon Fibrils
No full textModeling and measuring visco-elastic properties: From collagen molecules to collagen fibrils
No full textCollagen is the main structural protein in vertebrate biology, determining the mechanical behavior of
connective tissues such as tendon, bone and skin. Although extensive efforts in the study of the origin of
collagen exceptional mechanical properties, a deep knowledge of the relationship between molecular
structure and mechanical properties remains elusive, hindered by the complex hierarchical structure of
collagen-based tissues. Understanding the viscoelastic behavior of collagenous tissues requires knowledge
of the properties at each structural level. Whole tissues have been studied extensively, but less is
known about the mechanical behavior at the submicron, fibrillar and molecular level. Hence, we
investigate the viscoelastic properties at the molecular level by using an atomistic modeling approach,
performing in silico creep tests of a collagen-like peptide. The results are compared with creep and
relaxation tests at the level of isolated collagen fibrils performed previously using a micro-electromechanical
systems platform. Individual collagen molecules present a non-linear viscoelastic behavior,
with a Young's modulus increasing from 6 to 16 GPa (for strains up to 20%), a viscosity of 3.8470.38 Pa s,
and a relaxation time in the range of 0.24–0.64 ns. At the fibrils level, stress–strain–time data indicate
that isolated fibrils exhibit viscoelastic behavior that could be fitted using the Maxwell–Weichert model.
The fibrils showed an elastic modulus of 123746 MPa. The time-dependent behavior was well fit using
the two-time-constant Maxwell–Weichert model with a fast time response of 772 s and a slow time
response of 10275 s
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