31 research outputs found
Ultrastructure of human ovarian tissues and risk of cancer cells re-implantation after transplantation to chick embryo chorioallantois membrane (CAM) following vitrification or slow freezing
No full textOvarian follicle depletion and premature ovarian failure are significant challenges in cancer patients subjected to radio- or chemotherapy. Ovarian tissue (OT) cryopreservation would be an option when other fertility preservation methods are not accessible.
This study aimed to analyze the structure and ultrastructure of human OTs transplanted onto chick embryo chorioallantois membrane (CAM) after cryopreservation by vitrification or slow freezing. OTs from 10 cancer patients underwent cryopreservation. CAM transplantation was done on fresh and cryopreserved OTs, to assign samples to nine study groups as follows: 1) FI-FIII = fresh, 5- and 10-days post-CAM transplantation groups; 2) VI-VIII = vitrified, 5- and 10-days post-transplantation vitrified groups; 3) SFI-SFIII: slow frozen, 5- and 10-days post-transplantation slow freezing groups. Proliferation ability, folliculogenesis, and structural and ultrastructure were analyzed.
The density of primordial follicles did not change after both freezing methods, but reduced after 5 (P ≥ 0.05) and 10 days (P ≤ 0.05) post-CAM transplantation. The follicular grade significantly decreased in all transplanted tissues (P ≤ 0.0). The proliferation marker increased after cryopreservation, but reduced after transplantation (P ≤ 0.05). TEM evaluation showed better follicular ultrastructure in the fresh group, after transplantation. Stromal ultrastructure appeared more preserved after vitrification compared with slow freezing. There was no sign of malignant cell contamination after transplantation.
Some follicular TEM abnormalities were found in both methods of freezing, with a better transplantation rate after vitrification. Also, enhanced follicular activation resulted in faster follicular depletion in this method. The information regarding post grafting events would improve our knowledge for longer OTs' lifespans
Effective dosage of growth differentiation factor‐9β in folliculogenesis and angiogenesis in the sheep ovarian tissues grafted onto chick embryo chorioallantoic membrane
No full textAim: Scientists have tried to culture and transplant the ovarian tissues (OT), to preserve fertility in cancer
patients. However, one of the main limitations to the applicability of this technique is the folliculogenesis
disruption after transplantation. Due to the roles exerted by growth differentiation factor-9β (GDF9β), we
decided to determine the most effective dose of GDF9β on promotion of folliculogenesis and angiogenesis in
sheep OT grafted onto the chick chorioallantoic membrane (CAM).
Methods: Fresh sheep OT were grafted onto the CAM for 5 days, and divided into four groups based on
the addition of increasing doses of GDF9β (0, 150, 200 and 250 ng/mL). Following culture, histological
(hematoxylin and eosin [H&E] staining) and immunohistological studies (Ki-67) were done. Fibrotic and
necrotic regions were measured using MICROVISIBLE software. For comparing the follicle development
rates between the groups as well as differences in the Ki-67-positive follicles, analysis of variance was
applied.
Results: In both 200 and 250 ng/mL GDF9β groups, significantly higher rates of intermediary and primary follicles were observed, also the numbers of good quality follicles increased in the aforementioned
groups and the rates of fibrotic and necrotic areas decreased. Moreover, in the 200 and 250 ng/mL
GDF9β groups, the number of capillaries and the proliferative activity increased. The lower dose of
GDF9β (150 ng/mL) neither activated the primordial follicles nor lead to an increase in the number of
growing follicles.
Conclusion: Addition of high dosages of GDF9β to the OT, grafted onto the CAM resulted in higher
folliculogenesis and better transplantation features due to improvement in angiogenesis
Corrigendum to “The report of ovarian tissue transplant in Iran: A case report” [Int J Reprod BioMed 2024; 22: 323–328]
No full textThe publisher has been informed of an error that occurred on page 323 in which the third authors affiliation must be changed to Molecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. On behalf of the author, the publisher wishes to apologize for this error. The online version of the article has been updated on September 30, 2024 and can be found at https://doi.org/10.18502/ijrm.v22i4.16393
The Effect of Training in Virtual Environment on Nursing Students Attitudes toward Virtual Learning and its Relationship with Learning Style
No full textIntroduction: It is impossible to be successful in virtual training unless we consider individuals’ viewpoints toward it. Despite this fact, less attention has been paid to students’ attitudes at the end of a virtual course in the published studies. This study investigates the effect of a virtual training course on the students` attitudes toward virtual education and its relationship with learning styles.
Methods: This was a quasi-experimental study based on pre- and post- tests. Study population included undergraduate nursing students of second semester entered to Razi Faculty of Nursing and Midwifery of Kerman University of Medical Sciences in year 2009 and were studying the course titled “fluid and electrolyte disorders”. Students were divided into two groups of traditional or virtual learning, randomly. Course content was presented to the virtual group via website while traditional group were taught in classroom. Students’ attitudes toward virtual education were collected via a researcher-made questionnaire. Their learning styles were assessed by Gaziano & Liesen questionnaire. Data was analyzed by descriptive statistics (mean, standard deviation, and frequency distribution) and analytical statistics (independent t-test and paired t).
Results: Before training, the mean score (out of 5) of attitude toward virtual education was 3.19±0.48 in virtual group and 3.21±0.33 in traditional group the difference between mean scores was not significant. At the end of the semester, the score was 3.55±0.45 for virtual group and 3.21 ± 0.46 for traditional group which showed a statistically significant difference (p=0.03, t=2.15). The results of ANOVA revealed no significant relationship between learning style and attitude toward virtual training (p=0.07).
Conclusion: This study showed that passing a virtual training course could improve students’ attitudes toward virtual education. There is no difference in attitude toward virtual education among people with different learning styles
The effect of graphitic target density on carbon nanotube synthesis by pulsed laser ablation method
No full textWearing your destination: Making tourism experience accessible for people with travel hesitation by using smart glasses
No full textAbstract
This quantitative study is probably one of the primary efforts for offering a potential solution for people with short- and long-term travel hesitation through the lense of Self-Efficacy and Learned Helplessness Theories as well as Technology Acceptance Model (TAM). In this study, travel hesitation is divided to two segments of permanent and temporary. Permanent travel hesitation includes age restrictions, visual, hearing, body impairment, Autism, and any types of disabilities. Furthermore, temporary travel hesitation includes traveling cost, having a new-born, having any types of phobias, being afraid of COVID-19 or any other COVID variants, and having no desires and interest for travel.
The purpose of this study is to offer a solution for people with travel hesitations, and it includes three research questions. The data were collected from 344 sample size through Amazon Mechanical Turk (MTurk) in December 2021. The collected data were analysed through SmartPlS 3.0 software using PLS algorithm and bootstrapping techniques. This research findings verified that people with travel hesitation had willingness to use smart glasses for their travel purposes and also people’s intention to travel physically to a destination after trying smart glasses was increased.Embargo status: Restricted until 09/2027. To request the author grant access, click on the PDF link to the left
Evaluation of sheep ovarian tissue cryopreservation with slow freezing or vitrification after chick embryo chorioallantoic membrane transplantation
No full textComparison between open and closed systems for vitrification of individual sperm: assessing morphometric measurements and chromatin integrity
No full textAbstract Background Classic vitrification methods are not appropriate when there are minimal numbers of viable sperm, and the new methods emphasize the low semen volumes in these cases. The aim was to assess the efficacy of the cryotech as a device for freezing low sperm volume, through the two methods of open (OVS) and closed (CVS) vitrification systems. Methods Testicular biopsy samples from 30 men with obstructive azoospermia (OA) were assigned to three groups fresh control (FC), OVS, and CVS. Testicular sperms were selected using an ICSI injection pipette and vitrified on the cryotech straws, containing one droplet of freezing medium. After warming, sperm head morphometric characterizations were evaluated with the MSOME technique. Sperm motility, membrane integrity, chromatin quality assessment including DNA fragmentation, Chromomycine A3 staining (CMA3), and Aniline Blue (AB) were assessed. Fluorescein isothiocyanate-conjugated Pisum sativum agglutinin (FITC-PSA) was done to examine sperm acrosome integrity. Results The mean sperm motility, viability, and sperm with intact acrosome reduced after vitrification, in both methods of CVS, and OVS, but the results were more promising in the closed method (p < 0.05). However, the variations were not significant between the two methods of cryopreservation, the OVS undergoes significant head dimensions changes compared to the control group (p < 0.05). The results also showed higher membrane, and chromatin abnormality after OVS (p < 0.05). Conclusions The overall post-thaw recovery of human testicular sperm proposes that CVS is more efficient for single sperm cryopreservation, while higher sperm viability, and lower alterations in chromatin, acrosome, and sperm head morphometry were seen compared to OVS
Predictors of Quality of Life in Infertile Couples.
No full textOBJECTIVES:
This study was conducted with the aim of examines the quality of life of infertile couples and their relationship with the practical resilience of infertile couples referring to Yazd's centers of infertility.
METHODS:
This research is a descriptive-correlational study. The research population consisted of all infertile couples who referred to Infertility Centers in Yazd, Iran in the winter of 2016. Sampling was conducted in a non-random and accessible manner. The instrument used in the research included a) demographic information questionnaire, b) Conner and Davidson's Resilience Scale, and c) quality of life infertile couples questionnaire. Data were analyzed by SPSS software version 17 at a significant level of P < 0.05. To describe the data, descriptive statistics methods were used and the inferential statistics (Pearson correlation coefficient, regression, independent t test, and variance analysis) were used to test the research hypotheses.
RESULTS:
People (202 couples) participated in this research. Three variables of resilience (β = 0.04, P = 0.04), gender (β = -0.22, P < 0.001), and education level (β = 0.21, P < 0.001) had a prediction coefficient and there was a significant relationship with quality of life.
CONCLUSIONS:
This study showed that resilience, gender, and education predict the quality of life of infertile couples. In the infertile couples counseling program, resilience should be considered as a coping factor.
KEYWORDS:
Couples; Infertility; Quality of lif
The report of ovarian tissue transplant in Iran: A case report
No full textBackground: Cancer treatments such as chemotherapy and radiotherapy increase the chance of ovarian failure. Ovarian tissue transplantation (OTT) is a viable option for fertility preservation in these cases. We aim to report ovarian transplantation in a leukemia case undergoing the vitrification method.
Case Presentation: The case was a 28-yr-old female in Research and Clinical Center for Infertility, Yazd, Iran who was suffering from leukemia. Ovarian biopsy was performed by laparoscopy surgery and transported to cryopreservation lab at 4°C for 1–2 hr. The ovarian cortex was removed from the medulla, and ovarian strips were cryopreserved by vitrification. This procedure used the equilibration and vitrification solutions including medium 199 supplemented with 20% serum, and ethylene glycol and dimethyl sulfoxide with concentrations of 7.5% and 20%, respectively. Before doing OTT, we assessed the tissue viability and follicular count by chick embryo chorioallantoic membranes and histologic survey, respectively. OTT was done after complete remission, following warmed tissue sutured together and transplanted on the residual medulla on the right side. On the left side, the ovary was removed completely; however, 2 strips were put on the peritoneal pocket. Anti-Müllerian hormone, follicle-stimulating hormone, and luteinizing hormone levels were 0.1 ng/mL, 36.5 mIU/mL, and 19.8 mIU/mL before OTT. During a 6-month follow-up, the anti-Müllerian hormone increased to 0.9, and then follicle-stimulating hormone and luteinizing hormone levels decreased dramatically until 17.47 mIU/mL and 6.71 mIU/mL, respectively. Also, the patient had 3 cycles of menstrual periods.
Conclusion: We demonstrated an appropriate hormonal profile, and the restoration of the menstrual cycle might indicate a successful transplant. Further investigations are needed to achieve successful clinical outcomes.
Key words: Cryopreservation, Tissue transplantation, Leukemia, Vitrification, Ovarian follicle
