38 research outputs found
Recommended from our members
Documentation Related to a 1991 Observation of Sturgeon in the Rio Grande – Río Bravo, USA (Texas) and Mexico (Coahuila)
No full textThis digital archive provides a compilation of previously unpublished information regarding a 1991 observation of a live sturgeon (Family Acipenseridae) in the Rio Grande-Río Bravo of the USA and Mexico. Though a few specimens collected in the 19th century support occurrence of sturgeon in this river basin, lack of credible, recent records has often led to this species not being recognized as part of the basin’s native fish fauna, and certainly not part of its modern fish community.
The second and third authors of this document manage the Fishes of Texas Project (Hendrickson, Dean A., & Cohen, Adam E. (2015). Fishes of Texas Project Database (version 2.0). Texas Advanced Computing Center, University of Texas at Austin. http://doi.org/10.17603/C3WC70) and knew of the unpublished 1991 observation of sturgeon reported here. They requested the content provided here from first author (Platania) who provided what follows below (verbatim as received in April 2018) and permission to archive it for public access.Integrative Biolog
Έκφραση DAZL, OCT4 και Υποδοχέα Ωκυτοκίνης σε καινοτόμα καλλιέργεια αμνιακού υγρού
No full textΤα MSCs (Mesenchymal Stem Cells- Μεσεγχυματικά Βλαστικά κύτταρα) αποτελούν έναν πληθυσμό πολυδύναμων κυττάρων και εμφανίζουν ιδιότητες πολυδυναμίας και αυτοανανέωσης σε επιμέρους ιστούς της μεσεγχυματικής σειράς. Επιπλέον παρουσιάζουν την ικανότητα της in vivo αποκατάστασης του ιστού από τον οποίο προέρχονται. Επομένως τα MSCs αποτελούν μια σημαντική πηγή πολυδύναμων κυττάρων για τη συστηματική εφαρμογή αυτόλογης κυτταρικής και γονιδιακής θεραπείας. Συνεπώς, η μελέτη και ο προσδιορισμός εναλλακτικής πηγής MSCs κρίνεται απαραίτητος. Η παρούσα μελέτη επικεντρώθηκε σε μια καινοτόμο τεχνική καλλιέργειας του AF (Amniotic Fluid- Αμνιακού υγρού), στην οποία ο εμβρυϊκός ορός βοοειδών αντικαθίσταται με ορό αμνιακού υγρού, με σκοπό την ταυτοποίηση, την απομόνωση και τον χαρακτηρισμό κυττάρων AF-ΜSCs like (Amniotic Fluid Stem Cells - Βλαστικά κύτταρα Αμνιακού υγρού). Κύτταρα από ανθρώπινο αμνιακό υγρό καλλιεργήθηκαν και AF-MSCs like ταυτοποιήθηκαν με μικροσκοπία. Τα κύτταρα απομονώθηκαν και στη συνέχεις πραγματοποιήθηκε μοριακός έλεγχος της γονιδιακής έκφρασης των δεικτών πολυδυναμίας Oct-4, DAZL καθώς και του υποδοχέα της ωκυτοκίνης. Η μοριακή ανανάλυση με RT-PCR (Real Time Polymerase Chain Reaction- Αλυσιδωτή Αντίδραση Πολυμεράσης Πραγματικού Χρόνου) έδειξε υψηλή έκφραση του Oct-4 και σημαντική έκφραση του DAZL και του υποδοχέα της ωκυτοκίνης. Η έκφραση του δείκτη πολυδυναμίας Oct-4 υποδεικνύει την παρουσία AF-MSCs κυττάρων κατάλληλων για άμεση χρήση στην αναγεννητική ιατρική λόγω έλλειψης ορού ζωικής προέλευσης. Η έκφραση του δείκτη DAZL υποδηλώνει την παρουσία πληθυσμού κυττάρων παρόμοιων με γεννητικά κύτταρα. Οι υποδοχείς ωκυτοκίνης εκφράζονται σε αρχικά στάδια ανάπτυξης των θηλαστικών, υποδηλώνοντας ότι η ωκυτοκίνη μπορεί να εμπλέκεται στη διαφοροποίηση των βλαστικών κυττάρων σε γενετικές κυτταρικές σειρές σε πρώιμα στάδια ανάπτυξης θηλαστικών.Human mesenchymal stem cells (MSCs) constitute a multipotent cell population and are able to differentiate into various lineages of mesenchymal tissues. Studies have indicated the capability of MSCs for in vitro regeneration of their tissue of origin. It is evident that MSCs play an important role in the systematic application of autologous cell and gene therapy. Amniotic fluid (AF) represents a noncontroversial MSCs source. The present study, focused on an innovative technique of cultured AF, in which fetal bovine serum is replaced with amniotic fluid serum, to achieve the identification, isolation and characterization of AF stem cell like cells. Cells from human amniotic fluid were cultured and AF-MSCs like cells were identified with microscopy. Cells were isolated and analyzed for gene expression of pluripotency markers Oct-4, DAZL and oxytocin receptor. RT-PCR results indicated high expression of Oct-4 and a significant expression of DAZL and oxytocin receptor. The expression of pluripotency marker Oct-4 indicates the presence of AF-MSCs like cells suitable for direct use in regenerative medicine due to the fact of animal origin free serum. DAZL expression indicates the presence of germ cells like cells. Oxytocin receptors are expressed in early mammalian developmental stages, suggesting that oxytocin may be involved in the differentiation of stem cells into the germ cell lines at the very early stages of the mammalian development
3Δ βιοεκτύπωση ενάγγειων οστικών μοσχευμάτων με χρήση σνθρώπινων ενήλικων βλαστοκυττάρων και κυτταρο-καθοδηγητικών βιοϋλικών με μηχανική διέγερση
No full textCurrent medical practice still faces significant challenges in treating large bone defects caused by trauma or disease. The main causes of large graft failure are inner graft necrosis and lack of integration with the host tissue due to poor vascularization. Developing bone grafts that can restore vascular function to the regenerating bone tissue has been the most difficult aspect to address. This thesis explores the critical role of vascularization in enhancing bone tissue engineering outcomes. Acknowledging the potential of 3D bioprinting in bioengineering complex tissues, this study introduces a dual-layered 3D bioprinted vascularized bone model aiming to promote bone regeneration under physiological mechanical loading. The inner layer comprises a vascular-like matrix created from a nanocomposite ink of gellan gum, laponite, and platelet-rich plasma (PRP), with laponite serving as a carrier for the PRP-containing bioactive growth factors, along with Wharton-jelly mesenchymal stem cells. The outer layer features a bone-like matrix containing bone marrow mesenchymal stem cells encapsulated in a photocrosslinkable blend of poly(ethylene glycol) diacrylate (PEGDA), gelatin, and poly(vinyl alcohol). The electrically conductive poly(3,4-ethylenedioxythiophene) (PEDOT) is introduced in the outer layer to enhance the activity of voltage-gated channels, facilitating calcium ion flow across the cell membrane, amplifying the signal triggered by mechanical stimulation. Dynamic cell cultures performed in the presence of mechanical stimulation within a bioreactor were employed to mimic the physiological environment of native bone tissue. The synergistic cellular and molecular interactions of the dual-layered constructs significantly enhance the osteogenic and angiogenic differentiation due to paracrine signaling. Key markers of osteogenesis and angiogenesis showed significant upregulation in response to mechanical stimulation, particularly in the dynamic cultures. The complex bioprinted constructs demonstrated excellent in vitro biocompatibility, without observing any adverse foreign body reaction following in vivo subcutaneous implantation in mice. The mechanoresponsive biofabricated platform has great potential to promote vascularized bone regenerationΗ σύγχρονη ιατρική πρακτική εξακολουθεί να αντιμετωπίζει σημαντικές προκλήσεις στην αντιμετώπιση μεγάλων οστικών ελλειμμάτων που προκαλούνται από τραύματα ή ασθένειες. Οι κύριες αιτίες αποτυχίας μεγάλων μοσχευμάτων είναι η νέκρωση του μοσχεύματος και η έλλειψη ενσωμάτωσης με τον ιστό του ξενιστή λόγω κακής αγγείωσης. Η ανάπτυξη οστικών μοσχευμάτων που μπορούν να αποκαταστήσουν τη λειτουργία των αιμοφόρων αγγείων στον αναγεννώμενο οστικό ιστό αποτελεί τη μεγαλύτερη πρόκληση. Αυτή η διατριβή διερευνά τον κρίσιμο ρόλο της αγγείωσης στη βελτίωση των αποτελεσμάτων της μηχανικής οστικού ιστού. Αναγνωρίζοντας τη δυναμική της τρισδιάστατης βιοεκτύπωσης στη βιομηχανική σύνθετων ιστών, η παρούσα μελέτη παρουσιάζει ένα διστρωματικό τρισδιάστατο βιοεκτυπωμένο αγγειωμένο μοντέλο οστού, το οποίο στοχεύει στην προαγωγή της αναγέννησης του οστού υπό φυσιολογικές μηχανικές φορτίσεις. Το εσωτερικό στρώμα αποτελείται από μία αγγειακή μήτρα, φτιαγμένη από νανοσύνθετο μελάνι αποτελούμενο από κόμμι γκελλάνης, λασπολίτη και πλάσμα πλούσιο σε αιμοπετάλια (PRP), με τον λασπολίτη να λειτουργεί ως φορέας των αυξητικών παραγόντων που περιέχονται στο PRP, μαζί με μεσεγχυματικά βλαστοκύτταρα του ζελέ του Wharton. Το εξωτερικό στρώμα διαθέτει μία μήτρα ομοιάζουσα με οστό, που περιέχει μεσεγχυματικά βλαστοκύτταρα μυελού των οστών, εγκλωβισμένα σε φωτοδιασταυρούμενο μείγμα πολυ(αιθυλενογλυκόλης) διακρυλικού (PEGDA), ζελατίνης και πολυ(αλκοόλης βινυλίου). Το ηλεκτρικά αγώγιμο πολυ(3,4-αιθυλενοδιοξυθειοφαίνιο) (PEDOT) προστίθεται στο εξωτερικό στρώμα για την ενίσχυση της δραστηριότητας των διαύλων που ελέγχονται από την τάση, διευκολύνοντας τη ροή των ιόντων ασβεστίου μέσω της κυτταρικής μεμβράνης και ενισχύοντας το σήμα που προκαλείται από τη μηχανική διέγερση. Οι δυναμικές κυτταροκαλλιέργειες, οι οποίες πραγματοποιήθηκαν παρουσία μηχανικής διέγερσης μέσα σε ένα βιοαντιδραστήρα, χρησιμοποιήθηκαν για να μιμηθούν το φυσιολογικό περιβάλλον του φυσικού οστικού ιστού. Οι συνεργιστικές κυτταρικές και μοριακές αλληλεπιδράσεις των διστρωματικών κατασκευών ενισχύουν σημαντικά τη διαφοροποίηση σε οστεογενή και αγγειογενή κύτταρα λόγω παρακρινικής σηματοδότησης. Σημαντικοί δείκτες της οστεογένεσης και της αγγειογένεσης έδειξαν σημαντική αυξημένη έκφραση σε απόκριση στη μηχανική διέγερση, ιδιαίτερα στις δυναμικές καλλιέργειες. Οι σύνθετες βιοεκτυπωμένες κατασκευές επέδειξαν εξαιρετική βιοσυμβατότητα in vitro, χωρίς να παρατηρηθεί καμία ανεπιθύμητη αντίδραση ξένου σώματος μετά από υποδόρια εμφύτευση σε ποντίκια. Η μηχανοευαίσθητη βιοκατασκευασμένη πλατφόρμα έχει μεγάλο δυναμικό για την προώθηση της αγγειωμένης αναγέννησης οστού
A Doubly Fmoc-Protected Aspartic Acid Self-Assembles into Hydrogels Suitable for Bone Tissue Engineering
No full textHydrogels have been used as scaffolds for biomineralization in tissue engineering and regenerative medicine for the repair and treatment of many tissue types. In the present work, we studied an amino acid-based material that is attached to protecting groups and self-assembles into biocompatible and stable nanostructures that are suitable for tissue engineering applications. Specifically, the doubly protected aspartic residue (Asp) with fluorenyl methoxycarbonyl (Fmoc) protecting groups have been shown to lead to the formation of well-ordered fibrous structures. Many amino acids and small peptides which are modified with protecting groups display relatively fast self-assembly and exhibit remarkable physicochemical properties leading to three-dimensional (3D) networks, the trapping of solvent molecules, and forming hydrogels. In this study, the self-assembling fibrous structures are targeted toward calcium binding and act as nucleation points for the binding of the available phosphate groups. The cell viability, proliferation, and osteogenic differentiation of pre-osteoblastic cells cultured on the formed hydrogel under various conditions demonstrate that hydrogel formation in CaCl2 and CaCl2-Na2HPO4 solutions lead to calcium ion binding onto the hydrogels and enrichment with phosphate groups, respectively, rendering these mechanically stable hydrogels osteoinductive scaffolds for bone tissue engineering
Nurse-led physical health interventions for people with mental illness: an integrative review of international literature
No full textPublished online: 16 Aug 2024Background: People experiencing mental illness receive physical healthcare from nurses in a variety of settings including acute inpatient, secure extended care, forensic, and community services. hile nurse-led clinical practice addressing sub-optimal consumer physical health is salient, a detailed understanding and description of the contribution by nurses to physical health interventions in people experiencing mental illness is not clearly articulated in the literature. Aims: he aim of this integrative review is to describe the state of knowledge on nurse-led physical health intervention for consumers, focusing on nursing roles, nursing assessment, and intervention settings. Methods: A systematic search of six databases using Medical Subject Headings from 2001 and 2022 inclusive was conducted. he Mixed Methods Appraisal ool (MMA) was utilised for quality appraisal. Results: Seventy-four studies were identified as “nurse-led”. nterventions were most common among community settings (n=34, 46%). Nurses performed varied roles, often concurrently, including the collection of 341 physical health outcomes, and multiple roles with 225 distinct nursing actions identified across the included studies. A nurse as lead author was common among the included studies (n=46, 62%). However, nurses were not always recognised for their efforts or contributions in authorship. Conclusions: here is potential gap in role recognition that should be considered when designing and reporting nurse-led physical health interventions.Brenda Happell, Alycia Jacob, Trentham Furness, Alisa Stimson, Jackie Curtis, Andrew Watkins, Chris Platania-Phung, Brett Scholz, Robert Stanto
Responsive Quaternized PDMAEMA Copolymers with Antimicrobial Action
No full textIn this work, the antimicrobial action of partially quaternized poly(2-(dimethylamino)ethyl methacrylate) (PQDMAEMA) copolymers using different alkyl halides is presented. The poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) homopolymer was synthesized by group transfer polymerization, followed by the modification of its tertiary amine groups, using bromoethane, iodoethane, bromohexane and bromoethanol, to introduce permanent cationic, quaternary ammonium salt moieties, randomly distributed along the polymer chains. In all cases, the degree of quaternization was low, at ~10 mol%, as verified by proton nuclear magnetic resonance spectroscopy to preserve the thermo-responsive character of the PDMAEMA precursor polymer. The biocidal activity of the lightly quaternized PQDMAEMA copolymers against Escherichia coli and Staphylococcus aureus was evaluated by calculating the minimum inhibitory concentration (MIC) as well as the minimum bactericidal concentration (MBC) of the polymers and by comparing them to the respective values of the precursor non-quaternized PDMAEMA homopolymer. The antibacterial mechanism of action in the solution was studied by zeta potential measurements, scanning electron microscopy and protein leakage tests signifying the disruption of the outer membrane of the bacterial cells to release their periplasmic proteins
Guerra, Fascismo, Resistenza. Avvenimenti e dibattito storiografico nei manuali di storia
No full text2009 - 2010Nel 1996 il Ministro della Pubblica istruzione Luigi Berlinguer emana un decreto che modifica la
suddivisione annuale del programma di Storia, considerato che le vigenti disposizioni “riservano
generalmente all’ultimo anno di ogni ciclo lo svolgimento di un programma troppo esteso e tale da non
consentire comunque un adeguato sviluppo degli eventi succedutisi nel corso del Novecento; attesa altresì
l’esigenza pedagogico-culturale di dedicare un ampio spazio alla trattazione di avvenimenti recenti di
notevole rilievo storico-politico”
Autori ed editori cominciano a pubblicare, fin dal 1997, “nuovissime edizioni” di manuali “per i
nuovi programmi”. Ma, come sono fatti questi testi? Cosa contengono? In che modo viene trattata la storia
del Novecento? Qual è la loro impostazione didattica? Che rapporto c’è tra storia e storiografia? E, infine:
viene colta l’occasione offerta dal decreto del ministro Berlinguer per rinnovare lo studio e l’insegnamento
della storia?
L’analisi di 32 manuali di storia per i licei, pubblicati tra il 1997 e il 2009, può fornire qualche
risposta. Si è pensato analizzare capitolo dedicato alla Seconda guerra mondiale per comprendere il modo in
cui viene affrontato, in termini di avvenimenti e di dibattito storiografico, il periodo 1940-1940 e in
particolare il passaggio dalla guerra dichiarata alla guerra combattuta; la crisi del regime e la caduta del
fascismo; il 25 luglio e l’8 settembre 1943; la continuità ambigua del Regno del Sud e la rottura ambiziosa
della Repubblica Sociale Italiana; le stragi nazifasciste; il complesso fenomeno della Resistenza; il ruolo
degli Alleati e delle forze politiche, sociali, spirituali che agiscono nella società italiana; la Liberazione;
Piazzale Loreto; il sangue dei vinti e il sangue dei vincitori; la resa dei conti e i conti con il passato;
l’influenza di tutto questo sulla dissoluzione della Monarchia e sulla nascita della Repubblica.
L’analisi della struttura e dei contenuti dei manuali di storia è stata condotta anche in riferimento alle
opportunità offerte dalle tecnologie per la didattica. [a cura dell'Autore]In 1996, Luigi Berlinguer Minister of Education issued a decree amending the division's annual program of history, given that the existing provisions "generally reserved to the last year of each cycle the action of a program that was too long and does not allow, however, adequate development of the events that occurred during the twentieth century, also waiting for the educational and cultural needs to devote a large space to discussion of recent events of great historical importance-political "
Authors and publishers are beginning to publish, since 1997, "brand new editions" of books "for new programs." But, as these texts are made? What do they contain? How is it treated the history of the twentieth century? What is their teaching approach? What is the relationship between history and historiography? And, finally, is seized the opportunity offered by the Decree of the Minister Berlinguer to renew the study and teaching of history?
The analysis of 32 history textbooks for high schools, published between 1997 and 2009, may provide some answers. It is designed to analyze the chapter on World War II to understand the way in which
it is addressed, in terms of events and historical debate, the period 1940-1940 and in particular the transition from war declared war fought, the crisis of the regime and the fall of Fascism, 25 July and 8 September 1943; ambiguous continuity of the Southern Kingdom and the failure of the ambitious Italian Social Republic, the Nazi-Fascist massacres, the complex phenomenon of resistance, the role of the Allies and the political, social , spiritual act in Italian society, the Liberation; Piazzale Loreto, the blood of the vanquished and the victors of the blood, the yield of the accounts and accounts with the past, the influence of this on the dissolution of the monarchy and the birth of the Republic.
The analysis of the structure and content of history textbooks was conducted in reference to the opportunities offered by technology for teaching. [edited by Author]IX n.s
Κοινωνικό - ιστορική προσέγγιση των εκπαιδευτικών δομών πριν απο το Δημοτικό Σχολείο
No full textΗ Προσχολική Αγωγή στην Ελλάδα δεν έχει μακροχρόνια ιστορία. Η σημαντικότητα της ίδιας όμως είναι κάτι παραπάνω από σίγουρη όσον αφορά την ολόπλευρη ανάπτυξη των νηπίων. Η Προσχολική Αγωγή ξεκίνησε να αναπτύσσεται αρκετές δεκαετίες μετά την ίδρυση του νεοελληνικού κράτους και πέρασε από πολλά εμπόδια μέχρι να αναγνωριστεί επίσημα από το κράτος και να εισαχθεί ως βαθμίδα στην τυπική εκπαίδευση. Στην παρούσα διπλωματική εργασία λοιπόν επιχειρείται μια κοινωνικό – ιστορική προσέγγιση των δομών πριν από το Δημοτικό σχολείο από την ίδρυση του νεοελληνικού κράτους μέχρι τις αρχές του 21ου αιώνα. Η μεθοδολογία που επιλέχθηκε είναι ιστορική – ερμηνευτική καθώς σκοπός είναι να κατανοηθεί μέσα από τις ιστορικές πηγές η εξέλιξη του θεσμού σε σχέση με τις κοινωνικές αλλαγές κάθε περιόδου στην Ελλάδα.Pre - school Education in Greece has no long history. But its importance is something beyond certain as regard to the whole child’s development. Pre - school Education began to develop several decades after the founding of the Modern Greek state and overcame many obstacles until it was formally recognized and introduced as a step in formal education. Through this present thesis, the author attempts a social and historical approach of the structures before the elementary school from the establishment of the Modern Greek state until the beginning of the 21st century. The methodology chosen was historical – interpretive, taking advantage of the historical sources in order to present the evolution of the institution in relation to the social changes of each period
Analysis of intrinsic evolutionary factors leading to microendemic distributions in New Caledonian leaf beetles
No full textMicroendemicity, or the condition of some species having local ranges, is a relatively common pattern in nature. However, the factors that lead to this pattern are still largely unknown. Most studies addressing this issue tend to focus on extrinsic factors associated with microendemic distributions, such as environmental conditions, hypothesising a posteriori about underlying potential speciation mechanisms, linked or not to these conditions. Here, we use a multi-faceted approach mostly focusing on intrinsic factors instead, namely diversification dynamics and speciation modes in two endemic sibling genera of leaf beetles with microendemic distributions, Taophila and Tricholapita, in a microendemicity hotspot, New Caledonia. Results suggest that the diversification rate in this lineage slowed down through most of the Neogene and consistently with a protracted speciation model possibly combined with several ecological and environmental factors potentially adding rate-slowing effects through time. In turn, species accumulated following successive allopatric speciation cycles, possibly powered by marked geological and climatic changes in the region in the last 25 million years, with daughter species ranges uncorrelated with the time of speciation. In this case, microendemicity seems to reflect a mature state for the system, rather than a temporary condition for recent species, as suggested for many microendemic organisms.Work funded by the Spanish “Agencia Estatal de Investigación” and the European Regional Development Fund of the European Commission (Grants CGL2017-83324-P and PID2021-123668NB-I00) and associated predoctoral scholarship PRE2018-083172 to LP.Abstract
Introduction
Results
Discussion
Methods
Data availability
References
Acknowledgements
Funding
Author information
Ethics declarations
Additional information
Supplementary Information
Rights and permissions
About this article
CommentsPeer reviewe
Whey protein isolate hydrogels containing Cannabidiol sup-port the proliferation of pre-osteoblasts
No full text
