3,875 research outputs found
vsharma-next/CRYOWRF: CRYOWRF v1.0
No full textCRYOWRF v1.0 released for public use.
See
1. "Introducing CRYOWRF v1.0: Multiscale atmospheric flow simulations with advanced snow cover modelling", Submitted to "Geoscientific Model Development" for scientific background.
2. Sharma, Varun (2021). Reproducibility Dataset for CRYOWRF v1.0. EnviDat. doi:10.16904/envidat.232. for reproducing results in #1 and for using CRYOWRF for your own applications
vsharma-next/CRYOWRF: CRYOWRF v1.0
No full textCRYOWRF v1.0 released for public use. See 1. "Introducing CRYOWRF v1.0: Multiscale atmospheric flow simulations with advanced snow cover modelling", Submitted to "Geoscientific Model Development" for scientific background. 2. Sharma, Varun (2021). Reproducibility Dataset for CRYOWRF v1.0. EnviDat. doi:10.16904/envidat.232. for reproducing results in #1 and for using CRYOWRF for your own applications.CRYO
I Was Addicted to Sex With Married Women
No full textAs a young man, Akhil Sharma revelled in the most dangerous of liaisons, having sex with other men's wives – until the thrill began to pall
Supplementary Tables 1-4 for Jose, Sharma et al.
No full textThe data includes supplementary tables 1-4 for the manuscript, published as preprint below:Susan Jose, Himanshi Sharma, Janki Insan, Khushboo Sharma, Varun Arora, Sonam Dhamija, Nabil Eid, Manoj B. MenonbioRxiv 2023.08.27.554994; doi: https://doi.org/10.1101/2023.08.27.554994 </p
Maldives Resorts: Eco-Friendly Vacations
No full textLuxurious, exclusive and remote, the Maldives are the ultimate beach escape. They’re also a case study in the risks of global warming. Writer Akhil Sharma visits the country’s most eco-friendly resorts and discovers a remarkable cuisine worth protecting
Recollections of a Hindu Hedonist
No full textNovelist Akhil Sharma grew up in a teetotaling Indian household. Here, he tells how discovering a passion for great wine helped him create a new identity out of a painful past
The role of small non-coding RNAs in human T-cell leukemia virus type 1 (HTLV-1) infection and transformation
Get PDFHuman T-cell leukemia virus type 1 (HTLV-1) is the causative agent of two distinct pathologies, adult T-cell leukemia/lymphoma (ATLL), an aggressive neoplasm of mature CD4+ T-cells, and tropical spastic paraparesis/HTLV-1-associated myelopathy (TSP/HAM), a demyelinating neurodegenerative disease. The emerging importance of small noncoding RNAs in normal cell physiology and disease has prompted studies of their role in T-cell activation and transformation. The work described in the present thesis was aimed at understanding the role of small noncoding RNAs, in particular microRNAs and tRNA fragments (tRFs), in HTLV-1 infection and ATLL pathogenesis.
The laboratory generated small RNA libraries to identify the repertoire of small noncoding RNAs expressed in two HTLV-1-infected T-cell lines (C91PL and MT-2) compared to normal CD4+ T-cells. Results revealed upregulation of miR-34a in the cell lines. Many tRFs were identified in both uninfected and infected cells. One of the most abundant tRFs (tRF-3019) was derived from the 3’ end of tRNA-proline, which is considered to be the primer for HTLV-1 reverse transcriptase. Results of an in vitro reverse transcriptase assay verified that tRF-3019 was capable of priming HTLV-1 reverse transcriptase. Both tRNA-proline and tRF-3019 were detected in HTLV-1 virus particles. tRF-3019 may thus play an important role in HTLV-1 reverse transcription and could represent a target to control HTLV-1 infection.
Data from a microarray-based analysis of microRNA expression in ATLL samples compared to normal CD4+ T-cells revealed 21 downregulated microRNAs and 6 upregulated microRNAs. Upregulated microRNAs included miR-34a, which is a member of the highly conserved miR-34 family that acts as a tumor suppressor induced by p53 in other cell types. However, p53 is known to be functionally inactivated or mutated in ATLL cells and HTLV-1-infected cell lines. Treatment of infected cell lines with nutlin-3a, a drug that restores p53 activity by interfering with MDM2, resulted in an upregulation of miR-34a and strong downregulation of several of its predicted targets. These findings indicate that unblocking the p53 pathway in HTLV-1-infected cells promotes engagement of the miR-34a/mRNA regulatory network.
The final aim of the project was to identify microRNAs regulated by the viral regulatory protein Tax. To this end the HTLV-1-negative T-cell line Jurkat was transfected with a Tax expression plasmid and assayed for changes in mRNA and microRNA expression by quantitative RT-PCR. Results revealed significant alterations in the levels of 7 microRNAs in the presence of Tax. These included let-7g, whose levels were reduced in the Tax-expressing cells. Let-7g was also found to be downregulated in ATLL samples compared to normal CD4 cells analysed by microarrays, suggesting that this microRNA might play a tumor suppressor role in HTLV-1-mediated transformation. Experiments are currently underway to identify targets of let-7g in infected cells using as a starting point 14 genes identified by integrating results from microRNA target prediction programs with expression profiles for microRNAs and mRNAs in ATLL cells vs. CD4 controls
Aflibercept – How does it compare with other Anti-VEGF Drugs?
No full text<p>Aflibercept – How does it compare with other Anti-VEGF Drugs? Yog Raj Sharma*, Koushik Tripathy, Pradeep Venkatesh and Varun Gogia<br>Austin Journal of Clinical Ophthalmology</p>
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