37 research outputs found
Differentiation potential of human postnatal mesenchymal stem cells, mesoangioblasts, and multipotent adult progenitor cells reflected in their transcriptome and partially influenced by the culture conditions
No full textSeveral adherent postnatal stem cells have been described with different phenotypic and functional properties. As many of these cells are being considered for clinical therapies, it is of great importance that the identity and potency of these products is validated. We compared the phenotype and functional characteristics of human Mesenchymal Stem Cells (hMSC), Mesoangioblasts (hMab) and Multipotent Adult Progenitor Cells (hMAPC) using uniform standardized methods. Human MAPC could be expanded significantly longer in culture. Differences in cell surface marker expression were found among the three cell populations with CD140b being a distinctive marker among the three cell types. Differentiation capacity towards adipocytes, osteoblasts, chondrocytes and smooth muscle cells in vitro, using established protocols, was similar among the three cell types. However, only hMab differentiated to skeletal myocytes, while only hMAPC differentiated to endothelium in vitro and in vivo. A comparative transcriptome analysis confirmed that the three cell populations are distinct and revealed gene signatures that correlated with their specific functional properties. Furthermore, we assessed whether the phenotypic, functional and transcriptome features were mediated by the culture conditions. Human MSC and hMab cultured under MAPC conditions became capable of generating endothelial-like cells, whereas hMab lost some of their ability to generate myotubes. By contrast, hMAPC cultured under MSC conditions lost their endothelial differentiation capacity, while this was retained when cultured under Mab conditions, however myogenic capacity was not gained under Mab conditions. These studies demonstrate that hMSC, hMab and hMAPC have different properties that are partially mediated by the culture conditions.sponsorship: We thank Nele Peersman, Lotte Vanbrabant, and Ellen Konings for the excellent technical assistance with cell culture and RT-qPCR. We also thank Kristel Eggermont for the help with fluorescence microscopy. We are thankful to Dr. Anja Van Campenhout for providing us with the bone and skeletal muscle fragments. We thank Kris Van Den Bogaert for her critical review of this article to assure accuracy of the data. This work was supported by the Center of Excellence funding K.U.Leuven, an Odysseus award, research funding from Athersys Inc., and a grant from the European Commission (EC-FP6-STREP-STRO-KEMAP; to C.M.V.); the Center of Excellence funding K.U.Leuven (EF/05/13) (to A.L.); a CAF-DCF chair for stem cell research (to M.D.), and a OT (ETH-C0420-OT/09/053) and GOA (EME-C2161-GOA/11/012) grant from the K.U.Leuven (to C.M.V., M.S., and A.L.). V.D.R. is funded by a grant from the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen). S.C. is a research assistant of the Flemish Fund for Scientific Research (FWO Vlaanderen). S.W.V.G. and M.D. are senior clinical investigators of FWO Vlaanderen. (Center of Excellence funding K.U.Leuven|EF/05/13, Athersys Inc., European Commission|EC-FP6-STREP-STRO-KEMAP, CAF-DCF, K.U.Leuven|ETH-C0420-OT/09/053, K.U.Leuven|EME-C2161-GOA/11/012, Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT Vlaanderen))status: Publishe
The Efficacy of Working Memory Training on Working Memory Capacity, Psychopathology, and Mental Well-being
No full text__Abstract__
Mood and anxiety disorders are the most prevalent psychopathological disorders in
the Netherlands (de Graaf, ten Have, Van Gool, & Van Dorsselaer, 2012). Anxiety disorders
and major depression, which are the focus of this dissertation, affect respectively 10.1% and
5.2% of the Dutch population yearly. The World Health Organization (WHO) estimates that
150 million people worldwide suffer from depression (WHO, 2008) and 15% of the world
population will suffer from an anxiety disorder in his/her life (Kessler et al., 2009).
Approximately 85% of these patients suffer from both depression and anxiety (Gorman,
1996). This high comorbidity rate might indicate a shared underlying etiology and is
reflected in similar consequences. That is, both psychiatric disorders result in significant
social and personal concern, which is associated with reduced quality of life, productivity
and even significantly increased mortality (Doris, Ebmeier, & Shajahan, 1999). As a matter
of fact, mood and anxiety disorders are responsible for 40% of the costs for work absence
and disability to work (Hutschemaekers, Donker, & Richter, 2000). Unfortunately, the
prevalence of these disorders has remained stable over decades, which might imply that
contemporary treatments are not able to reduce these disorders (de Graaf et al., 2012)
Design of ovalbumin-loaded mesoporous silica nanoparticles as proof of concept for the development of personalized anti-cancer nanovaccines
No full textModulated electrohyperthermia as part of immunogenic cell death treatment in pediatric neuro-oncology
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Significance of crustal-scale shear zones and synkinematic mafic dykes in the Nagssugtoqidian orogen, SW Greenland: a re-examination
No full textCurrent concepts on oxidative/carbonyl stress, inflammation and epigenetics in pathogenesis of chronic obstructive pulmonary disease
No full textChronic obstructive pulmonary disease (COPD) is a global health problem. The current therapies for COPD are poorly effective and the mainstays of pharmacotherapy are bronchodilators. A better understanding of the pathobiology of COPD is critical for the development of novel therapies. In the present review, we have discussed the roles of oxidative/aldehyde stress, inflammation/immunity, and chromatin remodeling in the pathogenesis of COPD. An imbalance of oxidants/antioxidants caused by cigarette smoke and other pollutants/biomass fuels plays an important role in the pathogenesis of COPD by regulating redox-sensitive transcription factors (e.g., NF-κB), autophagy and unfolded protein response leading to chronic lung inflammatory response. Cigarette smoke also activates canonical/alternative NF-κB pathways and their upstream kinases leading to sustained inflammatory response in lungs. Recently, epigenetic regulation has been shown to be critical for the development of COPD because the expression/activity of enzymes that regulate these epigenetic modifications have been reported to be abnormal in airways of COPD patients. Hence, the significant advances made in understanding the pathophysiology of COPD as described herein will identify novel therapeutic targets for intervention in COPD
Multimodal immunotherapy integrated into standard therapy for GBM: learning from clinical practice
No full textImmunotherapy for malignant glioma: preclinical models to improve efficacy of the immune attack
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