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Sixth Symposium of the International Working Group on Plant Viruses with Fungal Vectors
Dal 5 al 7 Settembre presso l’Aula Prodi (P.zza S. Giovanni in Monte) si è svolto il sesto congresso internazionale del Working Group on Plant Viruses with Fungal Vectors (IWGPVFV). IWGPVFV è un gruppo internazionale di ricerca sui virus delle piante trasmessi dal terreno mediante vettori fungini e protozoi. Virus questi ultimi che sono responsabili di numerose malattie nelle colture cerealicole, industriali e orticole a livello mondiale.
Durante i tre giorni del convegno, a cui hanno partecipato circa 130 relatori provenienti dall’Europa, dall’Asia, dall’America, dall’Africa e dall’Australia, sono state discusse una settantina di ricerche riguardanti la caratterizzazione e tassonomia dei virus delle piante trasmessi da funghi e protozoi, la caratterizzazione biologica e molecolare dei vettori di tali virus, l’epidemiologia e il controllo delle malattie con particolare riguardo allo studio dei meccanismi di interazione ospite - patogeno e le fonti di germoplasma nell' ambito della resistenza alle virosi.
Il Convegno, patrocinato dall’Università di Bologna e sponsorizzato da UniboCultura e Promega Italia, è organizzato dalla Dott. ssa Concepcion Rubies Autonell, del DiSTA della Facoltà di Agraria dell’Università di Bologna e dal Dr Victor Vallega, C.R.A. Istituto Sperimentale per la Ricerca Cerealicola di Roma. All’organizzazione del convegno hanno collaborato la Dott.ssa Annamaria Pisi e il Dr. Claudio Ratti del DiSTA della Facoltà di Agraria dell’Università di Bologna.
Ulteriori informazioni possono riscontrarsi nei siti:
www.agrsci.unibo.it/iwgpvfv/
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www.rothamsted.bbsrc.ac.uk/ppi/Iwgpvfv/index.htm
Reaction of 31 cultivars of durum wheat and 36 cultivars of common wheat to wheat soilborne mosaic virus during 2003-04
HTML Version http://wheat.pw.usda.gov/ggpages/awn/51/ Edited by W.J. Raupp, Department of Plant Pathology, Kansas State University, Manhattan, KS 66506-5502 USA; financial arrangements made by Brett F. Carver, Oklahoma State University, Department of Agronomy, Stillwater, OK 74078, USA
Reaction to wheat soilborne mosaic virus of 85 cultivars of durum wheat during six seasons in northern and central Italy.
Serological responses of Italian cultivars of Triticum durum to Wheat spindle streak mosaic virus.
Trials were carried out over three seasons in a field with natural inoculum sources of Wheat spindle streak mosaic virus (WSSMV) to evaluate the response to the virus of 71 cultivars of durum wheat (Triticum durum Desf.). Nine of these cultivars were assayed over three seasons, 18 over two seasons and 44 in one season only. The DAS (Double Antibody Sandwich) ELISA absorbance values obtained for single cultivars in each season were indexed to allow comparisons between results obtained in different years. Cultivar Claudio, assayed over three seasons, showed the lowest overall mean ELISA index, and thus represents, at present, the safest choice for soils infected by WSSMV. Relatively low mean ELISA indexes were observed also for cultivars Colorado, Rusticano and Provenzal
Resistance to accumulation of Soil-borne cereal mosaic virus in eight cultivars of Triticum durum Desf.
The concentration of Soil-borne cereal mosaic virus (SBCMV) and Wheat spindle streak mosaic virus (WSSMV) was monitored during two seasons in the roots and shoots of eight cultivars of durum wheat (Triticum durum Desf.) grown in a field near Rome (Italy) with
natural inoculum sources of these viruses. SBCMV and WSSMV titers were determined using an amplified immunosorbent electron microscopy (ISEM) procedure and – in one season – also with A sandwich enzyme-linked immunosorbent assay (PAS-ELISA). Particles of SBCMV were detected in the roots and shoots of all cultivars, in both seasons and on most collection dates. The cultivars manifested wide differences in SBCMV particle oncentration maxima in both roots and shoots. Results showed that the defense mechanism mainly responsible for such differences was directed at SBCMV accumulation. Three of the cultivars classified as susceptible to SBCMV accumulation showed dubious signs of ther partial resistance to early root infection or – in one season - to upward SBCMV movement, but neither of the latter defense mechanisms, if indeed present, had appreciable effects on the ltimate virion concentration in roots and shoots of these cultivars. The amount of SBCMV particles in roots was closely correlated to that detected in shoots throughout the greater part of both seasons, suggesting the presence of a generalized resistance mechanism controlling virion accumulation in the plants as a whole. Reappraisal of previously published data suggests that at least
some of the cases of resistance to upward movement and to early root infection reported in the literature for SBCMV and for the losely related Soil-borne wheat mosaic virus (SBWMV) could also be explained in terms of generalized resistance to virus accumulation. Resistance to WSSMV could not be investigated due to the exiguous amount of this virus detected in most of the root and shoot samples examined. PAS-ELISA data proved markedly less informative than those obtained using ISEM for both SBCMV and WSSMV
Reaction of 33 cultivars of common wheat to to wheat soilborne mosaic virus in 2001-2002.
Reaction of 72 durum wheat cultivars to wheat spindle streak mosaic virus in central Italy
In Italy, wheat spindle streak mosaic was first detected in a field near Rome in 1985. Subsequently, WSSMV has been identified, either alone or in mixed infection with CSBMV, in approximately 20 other sites throughout the northern and central and regions of the country. Field trials in 1998–99, 2003–04, and 2006–07 at the Experimental Farm of the Cereals Research Institute near Rome in a field with natural inoculum sources of both WSSMV and CSBMV evaluated the reaction to WSSMV using DAS-ELISA
of 72 durum wheat cultivars marketed in Italy. Nine of these cultivars were grown over three seasons, 17 over two seasons, and 46 in one season only. The cultivars were grown in 10-m2, solid-seeded plots, distributed in the field according to a randomized
Table 2. Estimated mean effects of cereal soil-borne mosaic virus on 34 durum wheat cultivars with different disease severity grown in a field near Bologna, Italy, during 2006–07. Grain yield Plant height Kernel weight Disease Number loss reduction reduction Heading
severity of delay score cultivar
Reaction of 33 cultivars of durum wheat and 35 cultivars of common wheat to cereal soilborne mosaic virus during 2004-05
Reaction of seventy-one cultivars of Triticum durum Desf. to Wheat spindle streak mosaic virus in central Italy
Field trials were carried out in 1998/99, 2003/04 and 2006/07 at the Experimental Farm of the Cereals Research Institute near Rome in a field with natural inoculum sources of both Wheat spindle streak mosaic virus (WSSMV) and Soil-borne cereal mosaic Virus (SBCMV) to evaluate the reaction to WSSMV of 71 cultivars of durum wheat (Triticum durum ) marketed in Italy. Reactions to WSSMV were evaluated on foliar extracts using DAS (Double Antibody Sandwich) ELISA. Nine of these 71 cvs. were grown over three seasons, 18 cvs. over two seasons, and 44 in one season only. In each of the trials, the DAS-ELISA values obtained for single cultivars were indexed in order to allow comparisons between different years. Cultivar Claudio, assayed over three seasons, showed the lowest overall mean ELISA index (0.5%), and thus appears – at present - the safest choice for soils with WSSMV in Italy.
Relatively low ELISA indexes were observed also for cvs. Colorado, Rusticano and Provenzal, tested two seasons only, and for cvs. Tiziana, Italo, Ofanto and Dupri, assayed one season.
Cultivars Colorado, Provenzal, Tiziana and Dupri are of particular interest, in that they are known to possess also a high degree of resistance towards SBCMV in Italy, whereas the highly WSSMV-resistant cv. Claudio is quite susceptible to SBWM
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