111 research outputs found
The research of the phytochemical composition of the fruits of Rosa (Rosa L.) genus plants.
No full textR. Juodytė master‘s thesis. Supervisor Prof. Dr. Habil. Valdimaras Janulis; Lithuanian University of Health Sciences, Medical Academy, Faculty of Pharmacy, Department of Pharmacognosy, Kaunas. The title: The research of the phytochemical composition of the fruits of Rosa (Rosa L.) genus plants. The aim of the research: to determine the variation of the qualitative and quantitative composition of phenolic compounds of fruits samples of wild rose varieties grown in Lithuanian climatic conditions, and to evaluate the antioxidant activity of their extracts in vitro. The objectives of the research: to determine the conditions for extraction of phenolic compounds in fruit samples of wild rose varieties grown in Lithuania; to investigate the variation of hydroxycinnamic acid derivatives, flavonoids, total variation of phenolic compounds in fruit samples of wild rose varieties by spectrophotometric method; to evaluate the antioxidant activity of fruit samples of wild rose varieties grown in Lithuania in vitro by spectrophotometric method; to determine the qualitative and quantitative composition of phenolic compounds in fruit samples of wild rose varieties by high-performance liquid chromatography; The object and methods of the research: fruit samples of wild rose varieties grown in Lithuanian climatic conditions. The total content of phenolic compounds, hydroxycinnamic acid derivatives, flavonoids and antioxidant activity in vitro were determined using spectrophotometric methods. Quantitative and qualitative composition of phenolic compounds was determined by high-performance liquid chromatography. The results and conclusions of the research: The highest amount of total phenolic compounds were determined in the fruit samples of the Rosa pimpinellifolia L. “Single Cherry” (50.13±4.17 mg GRE/g, p<0.05) and were determined on 7 October (51.49±0.52 mg GRE/g, p<0.05), 23 September (51.4±1.72 mg GRE/g, p<0.05) and 9 September (45.84±1.22 mg GRE/g, p<0.05) in the fruit samples of the Rosa canina L. collected during the period of ripening. When using DPPH and FRAP methods, the strongest antioxidant activity was determined in the extracts of fruit samples of the Rosa pimpinellifolia L. variety “Single Cherry” and Rosa rugosa variety “Adam chodun”. The strongest antioxidant activity was determined in the extracts of fruit samples of the Rosa canina L. on 7 October and 23 September by the DPPH and FRAP method. Caffeic acid, chlorogenic acid, quercetin, quercitrin, (+)-catechin, (-)-epicatechin, (-)-epicatechin gallate, rutin, phlorizin, kaempherol-3-O-glucoside were identified by HPLC method
Demand Study for Medicinal Plant-Based Raw Materials and Products Exhibiting Vitamin P Effect
No full textMaster’s Thesis by Jolanta Andrijauskienė: “Demand Study for Medicinal Plant-Based Raw Materials and Products Exhibiting Vitamin P Effect” / Supervisor Prof. Habil. Dr. V. Janulis. Lithuanian University of Health Sciences, Faculty of Pharmacy, Departament of Pharmacognosy. – Kaunas. Purpose: The study is aimed at examining the range of and demand for medicinal plant-based raw materials and products exhibiting vitamin P effect. Objectives: 1. To assess patients’ knowledge about medicinal plant-based raw materials and products exhibiting vitamin P effect. 2. To discuss information sources from which patients learn about plant-based medicinal raw materials and products exhibiting vitamin P effect. 3. To identify factors that influence the choice of medicinal plant-based raw materials and products exhibiting vitamin P effect. 4. To identify plant-based medicinal raw materials and products exhibiting vitamin P effect that are used most frequently for therapeutic purposes and prevention of vein diseases. Research methods: A questionnaire consisting of 17 questions was developed as a research instrument for the purpose of the study. A total of 450 questionnaires were distributed, of which 402 were returned. The response rate is 89.33 percent. Descriptive and graphical methods were used to present study results. The data obtained during the survey was processed using the SPSS 17.0 package and Microsoft Office Excel 2007. The p value threshold for statistical significance was used to check the data which are considered statistically significant when p<0.05. Results and conclusions: 50.7 percent of the respondents indicated that vitamin P effect is related to the reduction of vascular permeability, whereas 49.0 percent of the survey participants reported that biologically active compounds exhibiting vitamin P effect relieve vascular spasms. One third of the respondents indicated that these substances reduce leg swelling. One third of the participants are aware what bioactive substances have vitamin P effect. 59.2 percent of the respondents reported learning about medicinal plant-based raw materials and products exhibiting vitamin P effect from pharmacy specialists. The respondents obtain the least knowledge from scientific literature and publications. For 50.5 percent of the respondents, the choice of medicinal plant-based raw materials and products exhibiting vitamin P effect is influenced by a pharmacist’s recommendation, whereas 48.4 percent of the respondents indicated their physician’s recommendation as the main factor affecting their choice. The most frequently used medicinal plant-based raw material is green tea (reported by 29.5 percent of the respondents), whereas the most frequently selected medicinal plant-based product exhibiting vitamin P effect is Detralex 500 mg tablets (64.2 percent). The most frequently consumed food supplements were Rutin plus C capsules (27.4 percent), Rutin tablets (25.3 percent), and Rutin + C tablets (23.2 percent). The leg gel that the respondents reported using most often was Venolgon (32.6 percent)
Qualitative and quantitative composition variability of phenolic compounds in American cranberry (Vaccinium macrocarpon Aiton) fruits grown in Lithuania
No full textThe object and methods of the research: the samples of fruits of cranberry cultivars, grown in Lithuania ('Baiwfay', 'Bergman', 'Bain', 'Drever', 'Holliston', 'Pilgrim', 'Searles', 'Woolman'), qualitative and quantitative composition variability was investigated, using HPLC and UHPLC methods. The aim of the research: to determine the qualitative and quantitative composition of phenolic compounds of fruits of cranberry, which was grow in Lithuania and to evaluate the antioxidant activity in vitro of their extracts. The objectives of the research: to determine the extraction conditions of anthocyanins from the samples of fruits of American cranberry. To determine the quantitative composition of phenolic compounds in different cultivars of American cranberry. To determine the antioxidant activity in vitro in the samples of different cranberry cultivars. To determine the qualitative and quantitative composition of phenolic compounds in different cultivar samples of American cranberry fruits by HPLC methodology. To evaluate correlation between cranberry fruit extract containing quantitative composition of total phenolic compounds and the antioxidant activity in vitro. To determine the qualitative and quantitative composition in different cultivar samples of American cranberry fruits by UHPLC methodology. The results and conclusions of the research: the best extraction conditions for anthocianins were determined to be when extraction time is 40 min, ethanol concentration is 70%, ultrasovic bath power is 452W. The highest determined total amount of: anthocianins was 8,54±0,04 mg/g (p<0,05), flavonoids 5,34±0,026mg RE/g (p<0,05), phenolic compounds 18,06±0,15mg/g (p<0,05), proanthocianidins 10,55±0,26 mg EE/g (p<0,05). The strongest antioxidant activity determinated by ABTS method was in 'Baiwfay' cultivar (193,63±1,3 μmol TE/g), by TFPH method in 'Drever' cultivar (125,65±2,79 μmol TE/g), by FRAP method in 'Bergman' cultivar (41,87±0,18 μmol TE/g), by CUPRAC method in 'Baiwfay'cultivar (493,86±21,33 μmol TE/g). 16 componds have been determined by HPLC method in witch malvidin-3-O-arabinoside was the dominant one. Using UHPLC method was determined, that the highest total amount of flavonol group was found in 'Searles' cultivar (370,38±12,07 mg/g)
Phenolics qualitative and quantitative composition and antioxidantactivity identification in apple tree (Malus domestica L.) leaves
No full textPhenolic compounds are commonly found in apple leaves as secondary plant metabolites. Most common phenolic compounds are flavonoids (phloridzin, epicatechin, catechin and others) and phenolic acids (caffeic acid, chlorogenic acid). For plants phenolics act as antioxidants, protective agents against harsh conditions. For human use phenolics are used as natural antioxidants due to their ability to scavenge free radicals, inhibit their formation processes and induce response to oxidative stress. Other beneficial effects are antimicrobial, anti-cancerogenic, anti-inflammatory and cardiovascular protective effect. The aim of this research was to evaluate total content of phenolic compounds, total content of flavonoid compounds and antioxidant activity of different apple cultivars (Ligol, Aldas, Lodel, Auksis) using spectrophotometry and HPLC. Methods: Apple leaf samples were lyophilised and extracted using ultrasonic sound assisted extraction. 70% ethanol was used as extractant, extraction time was 40 min and temperature – 60˚C. To determine total content of phenolic compounds expressed as gallic acid equivalent (mg/g) for absolutely dried lyophilisate in apple leaf extracts spectrophotometric Folin-Ciocalteu method was used. To identify total content of flavonoids expressed as rutin equivalent (mg/g) for absolutely dried lyophilisate more specific reaction with AlCl3 was used. Two spectrophotometric methods FRAP and DPPH were used to determine total antioxidant activity expressed as trolox equivalent (TEAC μmol/g) of ethanolic extracts. HPLC was used to identify phenolics and their quantity. Results: total content of phenolics in apple leaf ethanolic extracts varied from 98,77 mg/g GAE in ‘Auksis’ cultivar to 114,86 mg/g GAE in ‘Lodel’ cultivar. Total content of flavonoids in apple leaf ethanolic extracts varied from 31,91 mg/g in ‘Ligol’ cultivar to 39,6 mg/g in ‘Lodel’ cultivar. TEAC values obtained using DPPH method varied from 142,83 μmol/g in ‘Ligol’ cultivar to 147,94 μmol/g ‘Lodel’ cultivar. TEAC values obtained using FRAP method varied from 365,45 μmol/g in ‘Ligol’ cultivar to 445,64 μmol/g in ‘Aldas’ cultivar. Highest total amount of quercetin glycosides (28601,7μg/g of absolute dry weight) was found in ‘Aldas’ cultivar, lowest amount (16954,4 μg/g) was found in ‘Ligol’ cultivar. Total amount of chlorogenic acid varied from 480,1 μg/g in ‘Aldas’ to 1383,5 μg/g in ‘Auksis’. Total amount of phloridzin varied from 106006,4 μg/g in ‚Aldas‘ to 114425,4 μg/g in ‚Ligol‘ cultivar. Total amount of catechins varied from 567,0 μg/g in ‚Lodel‘ cultivar to 851,4 μg/g in ‚Ligol‘ cultivar
Study of quantitative composition of phenolic compounds in Lithuania grown apples from different rootstocks and cultivation systems
No full textTo evaluate quantitative composition and antioxidant activity of phenolic compounds in Lithuania grown apples from different rootstocks and cultivation systems
Phenolic Compounds and Antioxidant Activity composition research in Rowan bark and fruits.
No full textObject and methods: Extracts of Sorbus L. bark and fruits. Total phenolic compounds, proanthocyanidins, hydroxycinnamonic acid derivatives are determined for UV spectrophotometry. Phenolic compounds qualitative and quantitative configuration is established by high-performance chromatography. Antioxidant activity by spectrophotometric method using DPPH, ABTS, FRAP reagents. Aim: To investigate the composition of phenolic compounds entry Lithuania growing rowan fruits and bark samples. Objective: Set rowan bark and fruits total phenolics variation of spectrophotometry method. Set rowan bark and fruits phenolics qualitative and quantitative compositions of the high-performance chromatography. Perform rowan bark and fruits antioxidant activity of spectrophotometry. Results: The highest total phenolic compounds common rowan bark established on August 16 in sample (10,51 ± 0,006 per cent.), proanthocyanidins - September 13th in sample (4,92 ± 0,118 per cent.), hydroxycinnamonic acid - April 26th in sample (0,38 ± 0,006 per cent.). Rowan fruits largest phenolic compounds set of Swedish mountain ash, grown in Draugystės park (Kaunas city), in sample (2,36 ± 0,052 per cent.), rowan fruits of these compounds largest growing in Pauslajo forest (Kėdainiai district) (1,98 ± 0,138 per cent.) and a maximum hydroxycinnamonic acid content as determined by conventional rowan growing in Daumėnų forest (Panevėžys district) , the sample (1,74 ± 0,018 per cent.). Neochlorogenic acid, chlorogenic acid and isoquercitrin found in Rowan bark. Neochlorogenic acid, chlorogenic acid, caffeic acid, rutin, hyperoside, isoquercitrin and astragalin found in rowan fruits. The highest antioxidant activity of the cortex in the sample was gathered on 10 May (949,54 ± 3,47 micromoles TE/g), and fruit, dialed Kėdainiai region, in sample (299,96 ± 3,4 micromoles TE/g). Conclusions: Rowan bark and fruits can be a viable raw material for food supplements, enriched rowan bark and fruit extracts, production, and isolation of individual phenolic compounds
Evaluation of phytochemical composition of plants of genus hawthorn (Crataegus L.) growing in Lithuania
No full textPerilės (Perilla L.) rūšių ir varietetų auginimo, fitocheminės sudėties ir biologinio poveikio tyrimas
No full textPerilla L. are significant for multi-pharmacological effect. The aim of this work is to study Perilla L. growth and develop¬ment tendencies, productivity; composition of biologically active compounds and their variations during the vegetation period, and the biological effect of the extracts; to select perspective plants for cultivation in Lithuania and for production of medicinal preparations. The objectives of the Study: To investigate and determine growth dynamics of Perilla L. species and varieties during the vegetation period and to assess the influence of climate conditions on the vegetation rhythmic. To assess the quantity dynamics of Perilla L. species and varieties herbal medicinal raw material and to compare the productivity of Perilla L. species and varieties. To determine the quantity of the essential oil in Perilla L. species and varieties and to identify the chemotypes of the plants. To determine the qualitative composition and variation dynamics of the phenolic acids in Perilla L. species and varieties. To determine the composition and variation dynamics of the flavone complex in Perilla L. species and varieties. To determine the composition and variation dynamics of antho¬cyanidines in Perilla L. species and varieties. To assess the radical scavenging activity of the extracts of Perilla L. species and varieties raw materials. To research the effect of Perilla L. extracts on the oxidative phosphorylation in the rat heart mitochondria
Variation of quantitative composition of phenolic compounds in apples during ripening.
No full textThe aim of the research – to investigate variation of quantitative composition of phenolic compounds in Lithuania grown apples cultivars 'Connel Red' and 'Ligol' in apples during ripening and to evaluate the antioxidant activity of these extracts. The objectives of the research: 1. To determine variation of total content of phenolic compounds in apple cultivars 'Connel Red' and 'Ligol' apples during ripening. 2. To determine variation of quantitative composition of ascorbic acid in apple cultivars 'Connel Red' and 'Ligol' apples during ripening. 3. To investigate antioxidant activity of ethanolic apple extracts by performing DPPH, FRAP and TFPH methods of Lithuania grown apples cultivars 'Connel Red' and 'Ligol'. 4. To evaluate correlation between 'Connel Red' and 'Ligol' cultivars of apple extracts containing quantitative composition of phenolic compounds, flavonoids, proanthocyanidins and antioxidant activity. Object and methods: Apple fruits samples of cultivars 'Connel Red' and 'Ligol' were collected in 2013 on different phonological stages from June to November. The total content of phenolic compounds, flavonoids and proanthocyanidins in apples samples was performed using UV-VIS spectrophotometric method, ascorbic acid - titration with 2,6-dichlorphenolindophenol sodium salt solution. Antioxidant activity was assessed by DPPH, FRAP and TFPH spectrophotometric methods. Results: Total content of phenolic compounds in apples samples ethanolic extracts during ripening period varied from 7,20 mg GAE/g to 77,23 mg GAE/g in 'Connel Red' cultivar and from 8,12 mg GAE/g to 80,26 mg GAE/g in 'Ligol' cultivar. Total content of flavonoids varied from 0,81 mg RE/g to 3,27 mg RE/g in 'Connel Red' cultivar and from 0,63 mg RE/g to 3,61 mg RE/g in 'Ligol' cultivar. Total content of proanthocyanidins varied from 3,10 mg/g to 18,00 mg/g in 'Connel Red' cultivar and from 2,67 mg/g to 18,13 mg/g in 'Ligol' cultivar. Content of ascorbic acid varied from 0,029 percent to 0,058 percent in 'Connel Red' cultivar and from 0,031 percent to 0,058 percent in 'Ligol' cultivar. The highest TE values ('Connel Red' – 735,27 ± 25,38 μmol TE/g, 'Ligol' – 715,63 ± 24,19 μmol TE/g) in apple samples ethanol extract was assessed FRAP method. The strongest correlation (0,963 – 0,997) between phenolic compounds, flavonoids and proanthocyanidins content and antioxidant activity was estimated using FRAP method. Conclusion: During development and ripening stages of apples major phenolic compounds, flavonoids, proanthocyanidins and ascorbic acid were determined at the beginning of their stage of development (17 June). Therefore at this stage we recommend to carry out the set-ups of apple extracts production or extraction of individual compounds
Qualitative and quantitative determination of phenolic compounds composition in apple peels and pulp
No full textObject and methods: Apple peel and pulp extracts. Quantitative analysis of the total content of phenolic compounds, flavonoids and proanthocyanidins was assesed by spectrophotometry. Qualitative and quantitative determination of phenolic compounds was performed using high performance liquid chromatography. The antioxidant activity was assesed by ABTS, DPPH and FRAP spectrophotometric methods. Pectin content was determined by a gravimetric method. Aim: to determine the qualitative and quantitative composition of phenolic compounds of apple peels and pulp of apple cultivars grown in Lithuanian climatic conditions and to assess the antioxidant activity of their extracts. Objective: to determine of the phenolic compounds quantitative composition variation of apple peels and pulp of apple cultivars 'Ligol', 'Connel Red', 'Aldas', 'Auksis', 'Rajka' and 'Lodel'. To determine the qualitative and quantitative composition of phenolic compounds of apple peels and pulp of apple cultivars 'Aldas' and 'Lodel' using high performance liquid chromatography. To assess the antioxidant activity of apple peel and pulp extracts. To evaluate the correlation between the total phenolic, flavonoid and proanthocyanidin content in apple peels and pulp and the antioxidant activity of their extracts. To determine the pectin content in apple peels and pulp. Conclusions: among apple peels the highest amount of phenolic compounds (3,37±0,03%), flavonoids (0,24±0,003%) and proanthocyanidins (0,29±0,006%) were determined in the peels of cultivar 'Aldas'. Among apple pulp the highest amount of phenolic compounds (2,60±0,05%), flavonoids (0,03±0,001%) and proanthocyanidins (0,21±0,007%) were determined in the peels of cultivar 'Lodel'. 11 phenolic compounds were identified and quantified in apple peels and pulp of cultivars 'Aldas' and 'Lodel'. Chlorogenic acid, procianydin B2 and (-)-epicatechin were the main phenolic compounds found in both apple cultivars. The highest antioxidant activity in apple peels was determined in cultivar 'Aldas' (119,86±3,23 µmol TE/g) and the highest antioxidant activity in apple pulp was determined in cultivar 'Auksis' (77,43±3,56 µmol TE/g). The strongest correlation (0,953) was determined to be between the total amount of phenolic compounds and the antioxidant activity, determined by the DPPH method. The highest pectin amount was determined in cultivar 'Rajka' peels (13,3±0,5%) and pulp (16,2±0,6%)
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