2 research outputs found
Review of Research on Public Libraries: A Scopus Based Bibliometric Analysis
No full textPublic libraries are considered as peoples universities and can play a vital role in overall development of community they serve with the help of the resources and services. We have collected to data from the Scopus database for the period of 1971-2019. We have identified the trends in public libraries research, prominent authors who are involved in the research on public libraries issues and Y applied to evaluate the author’s contribution. Core journals and productive countries also determined and taken up the author supplied keyword analysis over decades. This work would be useful to the researchers who are working on the public libraries
Identification of Critical Motifs within HIV-1 Integrase Required for Importin alpha 3 Interaction and Viral cDNA Nuclear Import
No full textThe viral cDNA nuclear import is an important requirement for human immunodeficiency virus type 1 (HIV-1) replication in dividing and nondividing cells. Our recent study identified a specific interaction of importin alpha 3 (Imp alpha 3) with HIV-1 integrase (IN) and its involvement in viral cDNA nuclear import. In this study, we have performed a more detailed investigation on the molecular mechanism of how HIV-1 IN interacts with Imp alpha 3. Our results revealed a reduced interaction between the two IN mutants lNKK215,9AA(IN215,9) and INRK263,4AA (IN263,4) with Imp alpha 3, while an IN double mutant, IN215,9/263,4,was severely impaired for its Imp alpha 3-binding ability, even though it was still found interacting with other cofactors, IN interactor I and Transportin3. Immunostaining and fractionation analysis have shown that YFP-IN215,9/263,4 failed to localize in the nucleus of transfected cells. Also, we found that both major and minor nuclear localization signal binding grooves of Imp alpha 3 are involved in interaction with IN. All of these results suggest a cargo protein import receptor type of interaction. Finally, the effect of IN215,9/263,4 mutations on HIV-1 replication was evaluated, and real-time quantitative PCR analysis showed that, while mutant virus (v215,9/263,4) had a slightly lowered total viral DNA, the 2-long-terminal-repeat DNA, a marker for nuclear import, was greatly reduced during v215,9/263,4 infection in both dividing and nondividing cells. Also, by cell fractionation assay, we found that a significant proportion of viral cDNA was still retained in cytoplasmic fraction of v215,9/263,4-infected cells. Overall, our study provides strong evidence that (211)KELQKQITK and (262)RRKAK regions of IN C-terminal domain are required for Imp alpha 3 interaction and HIV-1 cDNA nuclear import. (C) 2011 Elsevier Ltd. All rights reserved.Biochemistry & Molecular BiologySCI(E)13ARTICLE5,SI847-86241
