1,721,012 research outputs found
Strong extracellular nuclease activity displayed by barley (Hordeum vulgare L.) uninucleate microspores
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Effetti della varietà e del substrato nella coltura di antere in orzo (Hordeum vulgare L.)
No full textEvaluation of genetic diversity between toxic and non toxic Jatropha curcas L. accessions using a set of SSR markers
No full textScepticism about Jatropha as a competitive biofuel feedstock especially on marginal soils has been growing; in fact, the jatropha-biofuel chain is risky economically and often financially unfeasible without significant government subsidies under these conditions. A valorization of the by-products and in particular of the extruded seed cake (about 70% (w/w) of the processed seed), as animal feed, currently prevented by the presence of phorbol esters (PE) toxins, could contribute to a significant improvement in the economic sustainability of the crop. Strategies for breeding improved varieties could be accelerated by DNA-based molecular marker technology. Wild Mexican accessions and accessions from other parts of the world (South America and Africa) were analyzed by 40 simple sequence repeat (SSR) markers. SSR primers were chosen on the grounds of their Tm, length, degree of polymorphism and specificity for toxic trait. The genetic study pointed out a high degree of similarity both within and among the non Mexican accessions. The Mexican accessions proved to be non toxic and genetically differentiated forming a well separated cluster from out of Mexico accessions. Some polymorphic loci were close correlated with the character toxicity and useful, once validated their association in segregating populations for Marker Assisted Selection (MAS)
Assessing diversity in common bean (Phaseolus vulgaris L.) accessions at phenotype and molecular level: a preliminary approach
No full textAssessing diversity across and within crop varieties is relevant to improve the description of collections in genebanks and in on-farm conservation. The differences among and within several common bena (Phaseolus vulgaris L.) landraces, collected from and maintained ex situ in NE Italy, were initially studied using a set of 15phenotypic descriptors. From a subgroups of five bean accessions, microsatellite genotyping was performed after the extraction of DNA from each of 38-40 individuals. One of the 15 phenotypical descriptors (plant architecture) was of no use. Only ten out of the 23 molecular markers did work, however sufficient to discriminate the accessions. The phenotypic descriptors identified a portion of the within-population variability. A few discrepancies were obtained when observations of phenotype descriptors were run independently in two alpine locations in NE Italy. The genetic approach carried out separately on DNA of about 40 individuals clarified the structure of the five accessions
Rapporti tra accrescimento dei germogli e sviluppo dei frutti anche in presenza di trattamenti con (GA3) nel pesco (Cv. "Redhaven")
No full textAFLP markers for the study of introgression between wild sunflower species (H.argophyllus and H. debilis ssp.cucumerifolius)and in relation with H.annuus
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