1,721,101 research outputs found
High-Resolution Confocal Imaging of Pericytes in Human Fetal Brain Microvessels
No full textPericytes are integral part of neurovascular unit and play a role in the maintenance of blood-brain barrier integrity, angiogenesis, and cerebral blood flow regulation. Despite their important functional roles, a univocal phenotypic identification is still emerging also for the lack of a "pan-pericyte" marker. In the present study, we describe in detail the method for performing fluorescence immunohistochemistry on thick free-floating sections from human fetal brain in high resolution laser confocal microscopy. This method enables to obtain three-dimensional images of pericytes and provides insights about their distribution and localization in the microvessels of human developing brain
INFILTRATING MYOEPITHELIOMA OF THE BREAST
No full textTumors exclusively composed of myoepithelial cells are extremely uncommon in the breast. We report such a tumor in a 25-year-old white woman. Clinical and ultrasonographic findings suggested a small benign lesion of the breast. Nodulectomy performed for intraoperative diagnosis found a malignant spindle cell tumor. Consequently, a quadrantectomy with axillary lymphadenectomy was carried out. Routine stains suggested a tumor of myoepithelial origin that infiltrated the fat tissue; immunocytochemical stains demonstrated strong cytoplasmic reactivity for actin, cytokeratin, S-100 protein, and vimentin. Glial fibrillary acidic protein (GFAP) was detectable only in scattered tumor cells. Examination of the resected breast tissue and lymph nodes did not reveal additional tumor foci. Due to the evidence of infiltrative growth in this case and to the uncertain prognosis for breast myoepitheliomas, we support the previously proposed term ''infiltrating myoepithelioma'' for this rare tumor. We also suggest that immunocytochemistry should be mandatory in every spindle cell tumor of the breast because this morphological pattern is shared by several tumors with different biological behaviors
Cx43 expression in the human microvascolature of the developing and adult human brain. It. J. Anat. Embryol. , ,
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Immunocytochemical detection of caveolin-1 by confocal microscopy in human brain. It. J. Anat. Embryol. :,
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Immunolocalization of GAP and tight junction proteins in the human developing brain microvessels
No full textEffects of 6-aminonicotinamide gliotoxin on blood-brain barrier differentiation in the chick embryo cerebellum.
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