1,721,012 research outputs found
Kinetics of radiation-Cytochrome C-induced modifications in liposomes analysed by FT-Raman Spectroscopy.
No full textNew insights into red-ox response modulation in Fanconi's anemia cells by hydrogen peroxide and glutathione depletors.
No full textFanconi's anemia (FA) patients face severe pathological consequences. Bone marrow
(BM) failure, the major cause of death in FA accounting for as much as 80-90% of
FA mortality, appear significantly linked to excessive apoptosis of hematopoietic
cells induced by oxidative stress. However as 20-25% of the FA patients develop
malignancies of myeloid origin. A surviving strategy for bone marrow and
hematopoietic cells under selective pressure has evidently taken place. This
study reports how lymphoblastoid cell lines derived from two FA patients display
significant resistance to oxidative stress induced by treatments with H(2) O(2)
and different glutathione (GSH) inhibitors which induce ROS, GSH depletion and
mitochondrial membrane depolarization (MMD). Among the various GSH inhibitors
employed FA cells appear peculiarly resistant to Menadione (5 μM) and Ethacrynic
acid (ETA, 50 μM), two drugs which specifically target mitochondria. Even after
pre-treatments with Buthionine Sulfoximine (BSO), a GSH synthesis inhibitor which
induces enhanced ROS induction, FA cells maintain significant resistance to these
stressors. These data suggest that the resistance to oxidative stress and the
altered mitochondrial and metabolic functionality found in the FA mutant cells
used in this study may define a survival strategy adopted in FA cells undergoing
transformation. The study of red-ox and mitochondria regulation in FA may be of
help in the diagnosis of the disease and in the care of the patients
Induction of kinetochore positive and negative micronuclei in V79 cells by the alkylating agent diethylsulfate
No full text
Analysis of the DNA contant distribution of micronuclei using flow sorting and fluorescent in situ hybridization with a centromeric DNA probe.
No full text
Identification and fate of a marker chromosome in metotrexate resistant V79,B7 cells by flow karyotyping and sorting, metaphase analysis and in situ hybridation.
No full textAntikinetochore antibodiesand flow karyotyping: new techniques to detect aneuploidy in mammalian cellsinduced by ionizing radiation and chemicals
No full textThe analysis of 10 potential spindle poisons for their ability to induce CREST-positive micronuclei in human diploid fibroblasts.
No full textChromosome rearrangements associated with CAD gene amplification. Experiments with cell hybrids.
No full text- …
