1,721,067 research outputs found
Detection of zoonotic genotype of Giardia duodenalis in viral immunodeficied cats in central Italy
Anoplocephala perfoliata infestation and colics in horses.
Intestinal distress represents one of the major cause of morbidity and mortality in horses
and is consequently, one of the most significant factors of economic loss for breeders. Since
the 1980’s several clinical reports have supplied a circumstantial evidence of a strong
association between the presence of a large number of Anoplocephala perfoliata specimens
and both specific surgical colics involving the ileo-caeco-colic tract and spasmodic colics,
leading to an increase in attention the veterinary practitioners on the dangerousness of this
tapeworm (Barclay et al. 1982; Beroza et al. 1983; Biglietti and Garbagnati 2002).
The effective role of A. perfoliata as primary pathogen of colic episodes requires further
investigation; however retrospective studies on this tapeworm infestation assessed its
predisposing role for intestinal ailments including ileo-caecal, caeco-caecal, caeco-colic
intussusceptions, ileal impactions and spasmodic colics (Beroza et al. 1986; Proudman and
Trees 1999).
On the basis of the results of our previous investigation both on tapeworm prevalence in
the equine population reared in Central Italy, showing a rate of 25.6 % (Veronesi et al.
2004) and on several colic events associated with A. perfoliata referred by veterinary
practitioners, the aim of the present work was to assess the relationship between such
helminthiasis and equine intestinal colic episodes.
The present survey was performed through the evaluation of the association between
different intestinal ailments and results of copromicroscopical and immunological
examinations on horses recovered at the Didactic Veterinary Hospital (OVUD) of the
Faculty of Veterinary Medicine of Perugia
Are macrocyclic lactones useful drugs for the treatment of Parascaris equorum infections in foals?
A study was carried out to assess the field efficacy of moxidectin (MOX) against Parascaris equorum in foals. A total of 70 foals from 2 Italian thoroughbred (Farms 1 and 2) stud farms and one trotter stud farm (Farm 3), aged 3-5 months and with faecal egg counts (FEC) f≥ 300 eggs per gram (EPG) were included in the study. On each farm, foals were divided into 2 groups (of 10, 8 and 10 foals, respectively) and treated with either moxidectin (MOX, 400 mcg/kg b.w.) or ivermectin (IVM, 200 mcg/kg b.w.) on day 0 and examined for faecal count reduction (FECR) on day 14. Five, 4 and 5 foals, respectively, were kept as untreated controls. An individually based estimation of efficacy was assessed by a bootstrap simulation to evaluate the percent reduction of FECRs. Two thousand bootstrap resamples were constructed from individual FECRs and the parasite population was considered susceptible for FECs ≥ 90% and 95% lower confidence limit (CL) > 90%, suspected resistant for FECRs ≥ 90% and 95% LCL 90%) in 7 and 5 trotter foals, respectively, and remained >90% until day 35. PYR treatment given on day 14 to remaining 8 foals (3 MOX-treated and 5 IVM-treated) was fully efficacious at faecal examinations carried out on days 28 and 35
Diffusione e caratterizzazione molecolare di isolati di Giardia duodenalis in cuccioli provenienti da canili del Centro Italia
Obiettivo del presente lavoro è stato quello di determinare la prevalenza di Giardia duodenalis in cuccioli ospitati presso canili,
definire alcuni potenziali fattori di rischio associati all’infezione e caratterizzare genotipicamente gli isolati per definirne
il potenziale zoonotico.
Lo studio ha coinvolto 127 cuccioli ospitati in 3 canili rifugio (C1,C2,C3). I campioni fecali di ciascun soggetto sono stati sottoposti
a screening mediante DFA per la ricerca di coproantigeni di G. duodenalis. I campioni risultati positivi sono stati testati
tramite PCR per l’amplificazione di frammenti target dei loci 16s-rRNA e β-giardina.
G. duodenalis è stata identificata in 49 dei 127 cuccioli (38,6%). I soggetti ospitati nella struttura da più di 21 giorni sono risultati
più frequentemente infetti rispetto agli altri, così come quelli ricoverati in box multipli. Dei 49 campioni DFA positivi,
36 isolati sono stati amplificati con successo: 22 con entrambi i targets indagati e 14 unicamente attraverso primers specifici
per il 16s-rRNA. Il sequenziamento dei 36 frammenti del 16s-rRNA ha permesso di evidenziare 31 assemblaggi di tipo
D, 4 di tipo C ed 1 di tipo A. Inoltre, l’analisi dei profili di restrizione degli amplificati della β-giardina ed il successivo sequenziamento
hanno permesso di rilevare la presenza di due “infezioni miste” (D ed A-I).
Nonostante la bassa prevalenza del genotipo zoonotico, le possibili implicazioni per la salute pubblica devono essere considerate
con la dovuta attenzione alla luce del fatto che i cuccioli sono la categoria preferenzialmente data in affidamento
presso nuclei familiari
Felid Lungworms and Heartworms in Italy: More Questions than Answers?
Nematodes infecting the cardiorespiratory system of cats have recently stimulated high scientific interest. Over the past few years, different aspects of these parasites have been clarified and various issues elucidated. This increased knowledge has improved awareness on this topic but, at the same time, some dilemmas have not been solved and new questions have been raised. In this review, we underline and discuss current knowledge of, and new doubts relating to, feline lungworms and heartworms, with the aim of stimulating new studies to fill gaps of basic (i.e., epidemiology and biology) and applied (i.e., clinical aspects) knowledge of the old and new parasites affecting the cardiorespiratory system of cats
Prevalence of Giardia intestinalis infectio and genotyping of isolates in puppies housed in kennels of Central Italy.
DISTRIBUTION OF HYDATIDOSIS IN SLAUGHTERED ANIMALS IN UMBRIA REGION FROM 1995 TO 2004: A RETROSPECTIVE ANALYSIS
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