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Norovirus detection in shellfish harvested and commercialized in Campania region: preliminary results
No full textNorovirus detection in shellfish harvested and commercialized in Campania regio
Applicazione di un protocollo One-step real time RT-PCR per l’identificazione di Norovirus in molluschi bivalve raccolti e commercializzati nella regione Campania
No full textDetection of Campylobacter from Poultry Carcass Skin Samples at Slaughter in Southern Italy
No full textCampylobacter is a major foodborne pathogen responsible for acute gastroenteritis characterized by diarrhea that is
sometimes bloody, fever, cramps, and vomiting. Campylobacter species are carried in the intestinal tracts of mammals and
birds, and sources of human infection include raw milk, contaminated water, direct contact with pets, and foods, particularly
poultry. Campylobacter jejuni and C. coli are the species that account for the majority of human infections. The aim of this
work was to determine the prevalence of Campylobacter in 190 poultry carcasses sampled at slaughter and to use a multiplex
PCR assay to determine if the isolates were C. jejuni or C. coli. C. coli was not isolated, while C. jejuni was recovered from
52 (37.1%) of 140 carcasses for which pools of four sampling sites (neck, cloaca, breast, and back) were examined. In the
remaining 50 carcasses, the four sites were analyzed separately, and C. jejuni was recovered from the samples in the following
order: neck (n 20), cloaca (n 16), breast (n 14), and back (n 11). The results are in agreement with those of other
studies, which showed that C. jejuni is more commonly associated with poultry than is C. coli. Control strategies for Campylobacter
should include interventions to eliminate C. jejuni in poultry at various stages of production and processing,
including at slaughter
NOROVIRUS MONITORING IN BIVALVE MOLLUSCS HARVERSTED AND COMMERCIALIZED IN SOUTHERN ITALY
No full textNorovirus (NoV) is the main cause of human nonbacterial gastroenteritis throughout the world. NoVs are classified into five genogroups: GI, GII, GIII, GIV, and GV. NoVs from GI and GII are the most commonly reported NoVs associated with human infections, and raw or undercooked shellfish have been identified as the main potential infection vehicle. European Commission Regulation 2073/2005 defines only bacteriological parameters for use as safety criteria for shellfish because reference methods for detection of viruses are lacking. From July 2007 to April 2010, 163 shellfish samples were collected in southern Italy from harvesting areas, authorized or nonauthorized retailers, and a restaurant after an outbreak of human gastroenteritis. The shellfish were analyzed for the presence of NoVs from GI and GII using the one-step real-time reverse transcription PCR protocol. A total of 94 shellfish samples (57.7%) were positive for the presence of NoV, and GII was the most frequently identified genogroup
Proteomics - Applications in Biology - Chapter title: Differentiation of four tuna species by two-dimensional electrophoresis and mass spectrometric analysis
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OCCURRENCE OF VIBRIO SPP. IN SEAFOOD OBTAINED FROM THE GULF OF NAPLES, ITALY. PRELIMINARY RESULTS.
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