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Insights into G1/S transition in plant
The G1/S transition generally represents the
principal point of commitment to cell division. Many of
the components of the cell cycle core machinery regulating
the G1/S transition in plants have been recently identified.
Although plant regulators of the G1/S transition display
structural and biochemical homologies with their animal
counterparts, their functions in integrating environmental
stimuli and the developmental program within cell cycle
progression are often plant-specific. In this review, recent
progress in understanding the role of plant G1/S transition
regulators is presented. Emerging evidence concerning
the mechanisms ofG1/S control in response to factors
triggering the cell cycle and the integration of these
mechanisms with plant development is also discussed
Immature embrios culture in Italian red chicory (Cichorium intybus L.)
Chicory (Cichorium intybus L.) embryo maturation in vitro was achieved in B5 medium without growth regulators.
Immature embryos were collected at different developmental stages 4–168 h after pollination. Only a few plantlets
were obtained from ovules collected shortly after pollination since the embryos at these early stages developed
abnormal leaves and roots which regenerated shoots ‘via’ organogenesis. Zygotic embryos collected 24–72 h after
pollination needed one month of culture in vitro before acclimation. Embryos collected at the heart or early torpedo
stage of development (three days after pollination) produced green plantlets after two weeks of in vitro culture and
thus they could be transferred to the greenhouse for acclimation. Culture of zygotic embryos in vitro has shown
to be a suitable technique to accelerate the breeding process in biennial cultivated Italian red chicory. In fact it
is possible to obtain plantlets ready for performing the selection for the desired agronomic traits one month after
pollination. Since flowering occurs after vernalization, the selection can be performed in the correct season before
flowering
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