1,721,891 research outputs found
Phytomanagement of trace element polluted fields with aromatic plants: supporting circular bio-economies
No full textTrace elements pollution of soils became a global concern because of their persistence in the environment which can lead to accumulation in food chains up to toxic levels. At the same time, there is a shortage of arable land for growing food, fodder and industrial crops, which highlights the need for remediation/use of polluted land. Restoration of degraded lands has been included as a vital component of UN Sustainable Development Goals (SDGs). We summarize various sources of entry of important trace elements in the environment, available biological reclamation and management strategies and their limitations. Recent advances in phytomanagement approaches using aromatic crops to obtain economically valuable products such as essential oils and revalorize such polluted areas are reviewed. The worldwide application of this strategy in the last 10 years is illustrated through a choropleth map. Finally, the emerging concept of phytomanagement as a restorative and regenerative circular bio-economy is also discussed.Fil: Saran, Anabel. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; ArgentinaFil: Much, Diego Gabriel. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; ArgentinaFil: Vangronsveld, Jaco. Hasselt University; BélgicaFil: Merini, Luciano Jose. Gobierno de la Provincia de la Pampa. Ministerio Publico. Agencia de Investigación Científica.; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentin
First high-quality draft genome of Ochrobactrum haematophilum P6BS-III, a highly glyphosate-tolerant strain isolated from agricultural soil in Argentina
No full textWe report here on a high-quality draft genome sequence of Ochrobactrum haematophilum strain P6BS-III (DSM 106071), a Gram negative, non-sporulating bacterium isolated from a pastureland (Buenos Aires province, Argentina) which had been chronically exposed to the herbicide glyphosate. The genome of 5.25 Mb with a DNA G+C content of 56.63% size was estimated to contain 5,291 protein coding genes and 57 RNA genes. Genome analysis revealed the presence of the phn operon, which is involved in the phosphonate degradation pathway, and a class II 5-enolpyruvylshikimate-3-phosphate synthase (EPSP) that confers tolerance to glyphosate. Genes related to plant growth promotion traits are also present, and include genes for phosphorus metabolism, calcium phosphate and phytate solubilization, siderophore production, organic acid biosynthesis and indole acetic acid (IAA) production.Fil: Massot, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Nanobiotecnología. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina. Hasselt University; BélgicaFil: Gkorezis, Panagiotis. Hasselt University; BélgicaFil: McAmmond, Breanne. Thompson Rivers University; CanadáFil: d´Haen, Jan. Hasselt University; BélgicaFil: Van Hamme, Jonathan. Thompson Rivers University; CanadáFil: Merini, Luciano Jose. Instituto Nacional de Tecnología Agropecuaria. Centro Regional La Pampa-San Luis. Estación Experimental Agropecuaria Anguil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vangronsveld, Jaco. Hasselt University; BélgicaFil: Thijs, Sofie. Hasselt University; Bélgic
Diversity and plant growth-promoting potential of (un)culturable bacteria in the Hedera helix phylloplane
No full textBackground: A diverse community of microbes naturally exists on the phylloplane, the surface of leaves. It is one of the most prevalent microbial habitats on earth and bacteria are the most abundant members, living in communities that are highly dynamic. Today, one of the key challenges for microbiologists is to develop strategies to culture the vast diversity of microorganisms that have been detected in metagenomic surveys.
Results: We isolated bacteria from the phylloplane of Hedera helix (common ivy), a widespread evergreen, using five growth media: Luria–Bertani (LB), LB01, yeast extract–mannitol (YMA), yeast extract–flour (YFlour), and YEx. We also included a comparison with the uncultured phylloplane, which we showed to be dominated by Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes. Inter-sample (beta) diversity shifted from LB and LB01 containing the highest amount of resources to YEx, YMA, and YFlour which are more selective. All growth media equally favoured Actinobacteria and Gammaproteobacteria, whereas Bacteroidetes could only be found on LB01, YEx, and YMA. LB and LB01 favoured Firmicutes and YFlour was most selective for Betaproteobacteria. At the genus level, LB favoured the growth of Bacillus and Stenotrophomonas, while YFlour was most selective for Burkholderia and Curtobacterium. The in vitro plant growth promotion (PGP) profile of 200 isolates obtained in this study indicates that previously uncultured bacteria from the phylloplane may have potential applications in phytoremediation and other plant-based biotechnologies.
Conclusions: This study gives first insights into the total bacterial community of the H. helix phylloplane, including an evaluation of its culturability using five different growth media. We further provide a collection of 200 bacterial isolates underrepresented in current databases, including the characterization of PGP profiles. Here we highlight the potential of simple strategies to obtain higher microbial diversity from environmental samples and the use of high-throughput sequencing to guide isolate selection from a variety of growth media.Funding
This work was supported by the Hasselt University Methusalem project 08M03VGRJ, by the project G0D0916N (“Plant–microbe associations to reduce particulate matter concentration and toxicity in urban areas: a multidisciplinary approach”) financed by the Research Foundation – Flanders (FWO) and by a personal grant of VS provided by the Research Foundation – Flanders (FWO). The funding bodies had no role in the design of the study and collection, analysis, interpretation of data, or manuscript preparation.
Acknowledgements
We thank Prof. Jonathan Van Hamme for proofreading the language in this manuscript. We thank the VSC (Flemish Supercomputer Center), funded by the Research Foundation – Flanders (FWO) and the Flemish Government – department EWI for providing the computational resources and services used in this work
Identification of bacterial populations in Brassica sp., Helianthus annuus and their possible role in heavy metal accumulation in plants
No full textThe Gentle Touch: supporting practical plant-based remediation solutions for contaminated sites
No full textProperties and Evolutionary Implications of the Heliobacterial Genome
No full textHeliobacteria are strictly anaerobic, anoxygenic phototrophic bacteria belonging to the phylum Firmicutes. They are distinct from other anaerobic anoxygenic phototrophs in that they produce unique photosynthetic pigments (bacteriochlorophyll g is the major pigment), have no capacity for autotrophic growth, and like their nonphotosynthetic relatives the clostridia, have a Gram-positive cell structure and are capable of producing heat-resistant endospores. Phototrophy in heliobacteria is carried out using an FeS-type (type-I) homodimeric reaction centre that represents the simplest known photosynthetic apparatus. We present herein a summary of the ecological, phylogenetic, photosynthetic, and physiological properties that distinguish heliobacteria from other phototrophs based on an analysis of features of the heliobacterial (Heliobacterium modesticaldum strain Ice1) genome. Also considered are the implications of pigment biosynthesis elements and components of the heliobacterial photosynthetic apparatus to the complex question of the origin and evolution of photosynthesis
Identification of bacterial populations in Brassica sp., Helianthus annuus and their possible role in heavy metal accumulation in plants
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