1,720,985 research outputs found
SCREENING FOR ANTIBODY REACTIVITY IN EARLY AXIAL SPONDYLOARTHRITIS IDENTIFIES NOVEL ANTIGENIC TARGETS
No full textSCREENING FOR ANTIBODY TARGETS AS NOVEL CANDIDATE BIOMARKERS FOR THE DIAGNOSIS OF ANKYLOSING SPONDYLITIS USING CDNA PHAGE DISPLAY
No full textIMMUNOGLOBULIN G ANTIBODIES TO THREE NOVEL PEPTIDES IN EARLY AXIAL SPONDYLOARTHRITIS IN TWO INDEPENDENT COHORTS
No full textIMMUNOGLOBULIN G ANTIBODIES TO THREE NOVEL PEPTIDES IN EARLY AXIAL SPONDYLOARTHRITIS IN TWO INDEPENDENT COHORTS
No full textSCREENING FOR ANTIBODY REACTIVITY IN EARLY AXIAL SPONDYLOARTHRITIS IDENTIFIES NOVEL ANTIGENIC TARGETS
No full textNovel maternal autoantibodies in autism spectrum disorder: Implications for screening and diagnosis
No full textIntroductionAutism spectrum disorder (ASD) is a complex neurodevelopmental disorder for which early recognition is a major challenge. Autoantibodies against fetal brain antigens have been found in the blood of mothers of children with ASD (m-ASD) and can be transferred to the fetus where they can impact neurodevelopment by binding to fetal brain proteins. This study aims to identify novel maternal autoantibodies reactive against human fetal brain antigens, and explore their use as biomarkers for ASD screening and diagnosis. MethodsA custom-made human fetal brain cDNA phage display library was constructed, and screened for antibody reactivity in m-ASD samples from the Simons Simplex Collection (SSC) of the Simons Foundation Autism Research Initiative (SFARI). Antibody reactivity against 6 identified antigens was determined in plasma samples of 238 m-ASD and 90 mothers with typically developing children (m-TD). ResultsWe identified antibodies to 6 novel University Hasselt (UH)-ASD antigens, including three novel m-ASD autoantigens, i.e., ribosomal protein L23 (RPL23), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and calmodulin-regulated spectrin-associated protein 3 (CAMSAP3). Antibody reactivity against a panel of four of these targets was found in 16% of m-ASD samples, compared to 4% in m-TD samples (p = 0.0049). DiscussionMaternal antibodies against 4 UH-ASD antigens could therefore provide a novel tool to support the diagnosis of ASD in a subset of individuals.This work was supported by the Research Foundation - Flanders [SB-FWO, 1S54717 (RM-C) and G098618N (PV)] and a Simons Foundation Autism Research Initiative - Explorer Award (SFARI, 534849).
The authors thank Dr. Marc Raes (Jessa Hospital) for help with m-TD recruitment, Kim Ulenaers, Veronique Pousset, and Anne Bogaers (Hasselt University, Biomedical Research Institute) for assistance with the sample collection, and Igna Rutten and Josianne Bleus (Hasselt University, Biomedical Research Institute) for their excellent technical support
SCREENING FOR IMMUNOGLOBULIN A ANTIBODY REACTIVITY IN EARLY AXIAL SPONDYLOARTHRITIS IDENTIFIES NOVEL PEPTIDE TARGETS
No full textSCREENING FOR ANTIBODY TARGETS AS NOVEL CANDIDATE BIOMARKERS FOR THE DIAGNOSIS OF ANKYLOSING SPONDYLITIS USING CDNA PHAGE DISPLAY
No full textAB0027 Screening for antibody reactivity in early axial spondyloarthritis identifies novel antigenic targets
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