10,935 research outputs found
Recommended from our members
Emmett L. Bennett, Jr. Offprint Collection
Full text linkThe scholarly library of Emmett L. Bennett, Jr. compiled in the course of his Editorship of the journal Nestor (founded in 1957). The collection includes scholarly publications (offprints) and manuscripts sent by prospective authors to Dr. Bennett. Includes a Finding Aid (PDF and Word) and Catalog (an Excel document for each of two record groups: offprints collected up to 1995, and offprints collected from 1995-2011). Both the Finding Aid and Catalog are provided to facilitate researchers' searches for offprints by author, title, journal, year, and subject.Classic
Phosphorylation of the phosphatase modulator subunit (inhibitor-2) by casein kinase-1. Identification of the phosphorylation sites.
No full textLetter from T.H. Hayes, Jr. to Attorney Henry M. Beaty Jr
No full textA letter of recommendation for Russell B. Sugarmon, Jr. to be admitted to the bar in Memphis and Shelby County. The author commends his ability, character, and family background
RAPID STIMULATION OF SER THR PROTEIN-KINASES FOLLOWING TREATMENT OF SWISS 3T3 CELLS WITH BOMBESIN - INVOLVEMENT OF CASEIN KINASE-2 IN THE SIGNALING PATHWAY OF BOMBESIN
No full textTreatment of quiescent Swiss 3T3 mouse fibroblasts with bombesin resulted in a rapid 6-8-fold stimulation of cytosolic Ser/Thr kinase activities toward the S6 peptide (RRLSSLR), myelin basic protein (MBP), and the G peptide (SPQPSRRGSESSEE). Anion exchange Mono Q chromatography resolved multiple S6 peptide- and G peptide kinase activities and two MBP kinase peaks. Both MBP- and several S6 peptide kinase peaks could be inactivated by PCSL (PP2A2) phosphatase action. This indicates that the bombesin-induced activation of these enzymes is mediated by a Ser/Thr phosphorylation event. The S6 peptide kinases as well as the two MBP kinases stimulated in response to bombesin are similar to those activated by epidermal growth factor in Swiss 3T3 fibroblasts which suggests that the early events of the signal transduction pathway mediated by these growth factors in Swiss 3T3 cells may converge in the activation of common Ser/Thr kinases. Bombesin, which acts as a sole mitogen for Swiss 3T3 fibroblasts, also produced a several-fold increase in the kinase activity toward the RRREEESEEE peptide, a specific substrate for CK-2. This kinase activity was heparin-sensitive and also measurable with the G peptide (SPQPSRRGSESSEE) and GS-1 peptide (YRRAAVPPSPSPSLSRHSSPHQSEDEE), which contain consensus sequences for phosphorylation by CK-2. The bombesin-stimulated CK-2 activity could not be measured in whole cytosols but was revealed by the anion exchange chromatography step. The activation of CK-2 was not reversed by PCSL phosphatase action. The implication of CK-2 in the signal transduction pathway of bombesin is discussed
The ATP,Mg-dependent protein phosphatase: regulation by casein kinase-1.
No full textAbstractThe free modulator subunit of the ATP,Mg-dependent phosphatase is phosphorylated up to 1 mol per mol by casein kinase-1, up to 1.85 mol per mol after dephosphorylation by the PCSH1 phosphatase, but 10-fold less when purified in the presence of NaF, suggesting an in vivo phosphorylation of the casein kinase-1 sites. Peptide mapping of 32P-modulator labeled by casein kinase-1 or -2 shows a different phosphorylation pattern. Phosphorylation of the inactive phosphatase by casein kinase-1 prevents the subsequent kinase FA-mediated activation, while it does not impair the activated phosphatase
A synthetic peptide substrate specific for casein kinase I.
No full textAbstractThe synthetic peptide, Asp-Asp-Asp-Glu-Glu-Ser-Ile-Thr-Arg-Arg, derived from the phosphorylation site of casein kinase-1 (CK-1) in β-casein A2, is readily phosphorylated by CK-1, but not by casein kinase-2 (CK-2), cyclic AMP-dependent protein kinase, protein kinsae C, phosphorylase kinase and protein kinase Fa. Phosphorylation by CK-1 occurs only at Ser-6, Thr-8 being unaffected. The Km for the peptide is higher (1 mM) than for β-casein A2 (40 μM), while the Vmaxis quite comparable. This is the first synthetic peptide substrate for CK-1 described so far, and can be used for the rapid and specific estimation of CK-1 activity in crude extracts
Phosphorylation of the modulator protein of the ATP, Mg-dependent protein phosphatase by casein kinase TS. Reversal by PCS phosphatases and control by distinct phosphorylation site(s).
No full textAbstractThe phosphorylation by casein kinase TS (II) of the modulator protein of the ATP,Mg-dependent phosphatase increases after preineubation with the PCSH1 phosphatase or with the catalytic subunit of the ATP,Mg-dependent phosphatase. Dephosphorylation by the two phosphatases combined leads to the incorporation of 2 mol phosphate per mol modulator (at Ser residues). Occupancy of the ATP,Mg-dependent phosphatase phosphorylation site(s) is a negative determinant in the phosphorylation of the modulator by kinase TS. Among the PCS phosphatases PCSh1 shows the highest activity toward the 32P-Ser residues labeled by kinase TS in untreated or previously dephosphorylated modulator, while the ATP,Mg-dependent phosphatase is totally ineffective. Protamine stimulates all phosphatase activities, so that the catalytic subunit of the ATP,Mg-dependent phosphatase becomes almost as effective as the PCSC phosphatase in dephosphorylating the kinase TS sites
Letter from Robert J. Walsh Jr., Chief, Freedom of Information/Privacy Office, Department of the Army, to Michi Weglyn, July 23, 1990
No full textA letter from Robert J. Walsh Jr., Chief, Freedom of Information/Privacy Office, Department of the Army, to Michi Weglyn. The letter is a response to Weglyn's 1988 Freedom of Information Act (FOIA) request to the Federal Bureau of Investigation (FBI), regarding records on the Japanese American Citizenship League (JACL).These materials are from box 73 and 74 of the Frank Chin Papers. The Frank Chin Papers contain personal and professional correspondence between Frank Chin and Michi Weglyn relating to particular projects on which either author was working as well as files related to the Day of Remembrance Tribute to Michi Weglyn
Visiting author Dr. Arthur Schlesinger, Jr. at MU
No full textVisiting author Dr. Arthur Schlesinger, Jr. at MU , b&w. Schlesinger wrote a book on disuniting America.https://mds.marshall.edu/parthenon_photo_morgue/1756/thumbnail.jp
Visiting author Dr. Arthur Schlesinger, Jr. being interviewed at MU
No full textVisiting author Dr. Arthur Schlesinger, Jr. being interviewed at MU , b&w. Schlesinger wrote a book on disuniting America.https://mds.marshall.edu/parthenon_photo_morgue/1757/thumbnail.jp
- …
