1,720,980 research outputs found

    Influence of supplemental dietary taurine on antibody production in broiler chicks

    No full text
    The objective of the present study was to investigatge the effects of supplemental dietary taurine on antibody production in broiler chicks. The result showed that the supplemetary dietary taurine, added in a dose of 0.6% but not of 0.3% exerted a positive influence on chicken antibody production to NDV an Pa

    Application of quantitative real-time PCR in the detection of prion-protein gene species-specific DNA sequences in animal meals and feedstuffs

    No full text
    Restriction site analysis of Polymerase Chain Reaction (PCR) products of cytochrome b mitochondrial DNA was applied to identify species in meat meal and animal feedstuffs. PCR was used to amplify a variable region of cytochrome b mitochondrial DNA gene. Species differentiation was determined by digestion of the obtained 359 bp amplicon with restriction enzymes, which generated speciesspecific electrophoresis patterns; the sequencing of PCR products was used as confirming analysis. PCR-RFLP analysis revealed the presence of meat meal in animal feedstuffs and distinguished species of interest. The results supported the application of the method in control measures which should be adopted for meat-meal-based animal feed, as suggested by EU law. As a technical improvement, to simplify the analysis, the number of enzymes presented in this study for the detection of different species was smaller than others described in the literature; discrimination between ruminant and nonruminant species and between mammalian and poultry species was possible with few digestions
    corecore