1,720,984 research outputs found
Reaction of 81 cultivars of common wheat in northern Italy to wheat soilborne mosaic virus during five seasons.
Le virosi più diffuse
I cereali autunno vernini sono soggetti a numerose malattie causate da virus, alcune delle quali costituiscono un importante fattore limitante per la loro produzione, sia sotto l’aspetto quantitativo che qualitativo. Le virosi possono assumere sviluppo epidemico in relazione alle caratteristiche delle popolazioni della pianta ospite, delle popolazioni del patogeno (natura della specie patogena, presenza di patotipi più o meno virulenti, modalità di trasmissione) in relazione alle condizioni ambientali (temperatura, precipitazioni, natura e stato del suolo, ecc)
Le cultivar resistenti al mosaico comune. I risultati di cinque anni di prove. Saggiate 81 varietà.
Reaction of cultivars of Triticum aestivum L. to Wheat spindle streak mosaic virus and to Soilborne cereal mosaic virus
Indexed data for comparing the reaction of durum wheat cultivars to Soil-borne cereal mosaic virus assayed in different seasons.
Evaluating the reaction to Cereal soilborne mosaic virus (CSBMV) of wheat cultivars in replicated, large-plot, field trials in which genotypes are compared in terms of symptom severity and ELISA values, as well as on the basis of agronomical performance has furnished reliable data, useful for both wheat growers and plant breeders.
Indexed data for fifteen field trials carried out in eight seasons and involving a total of 215 cultivars of durum and hexaploid wheat are presented. As might be expected, use of indexes did not appreciably modify the reaction thus far attributed to single cultivars. On the other hand, use of such indexed data has furnished more objective data for ranking cultivar reactions to CSBMV in Italy, and will enable more precise comparisons with data produced by research groups in other countries
Molecular characterization of Italian soilborne cereal mosaic virus isolates
A mosaic disease of winter wheat was first described in the USA by McKinney in 1925 and subsequently in many other wheat-growing countries. Until recently, the disease was associated worldwide to wheat soil-borne mosaic virus (WSBMV). Results of sequence analysis by German and Chinese researchers, however, have prompted the ICTV to approve a taxonomic proposal to divide American, European, and Chinese isolates into different species within the genus Furovirus. The novel species have been denominated wheat soil-borne mosaic virus, cereal soilborne mosaic virus (CSBMV), and wheat Chinese mosaic virus (WCMV). On the basis of the new classification, the wheat mosaic disease is believed to be generally caused by WSBMV in United States, Brazil, and Canada; by CSBMV in Europe; and by WCMV in Asia. Twenty-four wheat samples showing typical Furovirus symptoms were collected from farmers in fields northern, central, and southern Italy to study their degree of genetic diversity.
ssRNA was extracted, and the nucleotide sequence of a viral coat protein gene was determined for each sample. Nucleotide and amino-acid sequences alignment between the sequences obtained and the published RNA2 CP sequences of CSBMV, WSBMV, and WCMV isolates was generated applying the Clustal V method, and phylogenetic distance trees were constructed. Branching orders identified three major phylogenetic groups. In the first group, all the Italian isolates clustered closely together along with French, English, and German isolates (SBCMV species), whereas the second group included the American isolates, and the third ascribed the Asian isolates. Sequence data analysis revealed a high degree of genetic identity among Italian isolates (96.6 to 100.0 %) and between Italian isolates and SBCMV accessions
from the United Kingdom, France, and Germany (88.7 to 99.6 %). Sequence divergences from 29.6 to 45.9 % were observed between Italian SBCMV isolates and SBWMV or CWMV isolates. Amino-acid sequence analysis of CP cistron revealed few nonspecific exchanges as well as a high degree of sequence identity (97.7 to 100%) among CSBMV isolates from Italy and other European countries. Amino-acid sequence similarity between Italian and Asian or American isolates ranged from 71.0 to 81.2 %. Eventhough all the Italian isolates so far analyzed belong to the novel CSBMV species,
the presence of SBWMV in this country cannot be excluded
Molecular characterization of italian soil-borne cereal mosaic virus (SBCMV) isolates
Soil-borne cereal mosaic virus (SBCMV) was reported to affect wheat crops in Italy in 1960. Subsequently in many other European countries too. SBCMV causes a serious disease of wheat and growing resistant varieties represents the only economical means of control. Several wheat samples showing typical Furovirus symptoms were collected in Italy during the 2003 and 2004 seasons. Molecular characterization of the viral coat protein gene was carried out to study the degree of genetic diversity of these viruses over a relatively wide geographic area. Sequence data analysis revealed a high degree of genetic identity among Italian isolates, and between Italian and others SBCMV accessions. Alignment of deduced CP amino acids sequences showed an homology close to 100% between European isolates. Our results confirm a low degree of genetic diversity between European Furoviruses infecting wheat
- …
