195 research outputs found
From research to clinical practice: a systematic review of the implementation of psychological interventions for chronic headache in adults
No full textBackground: Psychological interventions have been proved to be effective in chronic headache (CH) in adults. Nevertheless, no data exist about their actual implementation into standard clinical settings. We aimed at critically depicting the current application of psychological interventions for CH into standard care exploring barriers and facilitators to their implementation. Secondarily, main outcomes of the most recent psychological interventions for CH in adults have been summarized. Methods: We conducted a systematic review through PubMed and PsycINFO in the time range 2008-2018. A quality analysis according to the QATSDD tool and a narrative synthesis were performed. We integrated results by: contacting the corresponding author of each paper; exploring the website of the clinical centers cited in the papers. Results: Of the 938 identified studies, 28 papers were selected, whose quality largely varied with an average %QATSDD quality score of 64.88%. Interventions included CBT (42.85%), multi-disciplinary treatments (22.43%), relaxation training (17.86%), biofeedback (7.14%), or other interventions (10.72%). Treatments duration (1 day-9 months) and intensity varied, with a prevalence of individual-basis implementation. The majority of the studies focused on all primary headaches; 4 studies focused on medication-overuse headache. Most of the studies suggest interventions as effective, with the reduction in frequency of attacks as the most reported outcome (46.43%). Studies were distributed in different countries, with a prevalent and balanced distribution in USA and Europe. Ten researches (35.71%) were performed in academic contexts, 11 (39.28%) in clinical settings, 7 (25%) in pain/headache centres. Interventions providers were professionals with certified experience. Most of the studies were funded with private or public funding. Two contacted authors answered to our e-mail survey, with only one intervention implemented in the routine clinical practice. Only in three out of the 16 available websites a reference to the implementation into the clinical setting was reported. Conclusion: Analysis of contextual barriers/facilitators and cost-effectiveness should be included in future studies, and contents regarding dissemination/implementation of interventions should be incorporated in the professional training of clinical scientists. This can help in filling the gap between the existing published research and treatments actually offered to people with CH
«Una realtà di puri echi»: Simbolo e comunione in Pirandello, chi? Di Amelio Memè Perlini
No full textLa linea teatrale degli anni Settanta, come scrive Valentina Valentini, caratterizzò il Nuovo Teatro italiano con la tendenza a espandersi oltre i confini geografici e disciplinari e, al contempo, con la tensione verso un nucleo comune (corpo, gesto, movimento) per «riparare nel luogo teatrale come negli spettacoli del Teatro-Immagine (secondo la definizione data da Bartolucci) di Mario Ricci, Memé Perlini, Giancarlo Nanni, Giuliano Vasilicò». Di fatto, è un processo di permeazione quello compiuto da Memé Perlini con il testo Pirandelliano, nell’accezione latina di per- «attraverso» e meare «passare». Sei personaggi in cerca d’autore per lui rappresentò una doppia illuminazione: una per così dire pubblica, riguardo al suo modo di vedere la scena, l’altra inerente a un’infelicità personale e collettiva che «gli riempiva i giorni e le notti» e che mutuò nella scrittura di Pirandello chi?, messo in scena per la prima volta nel 1973, come già accennato, al Beat ’72 di Roma. Perlini traspose la frammentarietà che caratterizzava la società di quegli anni in quelli che definiva frammenti-immagini, volti a ridurre il corpo dell’attore sulla scena in sezioni di luce e buio, nell’idiosincratica spinta a «parcellizzare, frantumare gli oggetti teatrali (paura del corpo) ma anche chiarissimo tentativo di ricostruirli (desiderio di rappresentare)».The direction of theatre of the 1970s, as Valentina Valentini writes, characterized New Theatre in Italy with its tendency to expand beyond geographic and disciplinary borders and, at the same time, with its tension towards a common core (body, gesture, movement) to «find shelter in the place of theatre like in the productions of Teatro- Immagine (as defined by Bartolucci) by Mario Ricci, Memè Perlini, Giancarlo Nanni, Giuliano Vasilicò». Memè Perlini’s work on Pirandello’s play was in fact a process of permeation, in the Latin sense of per- «through» and meare «transit». Six Characters in Search of an Author by Pirandello represented a double enlightenment for him: one that could be termed public, concerning his way of understanding the stage, the other inherent to a personal and collective unhappiness that «filled his days and nights» and which he wove into the writing of Pirandello chi?, staged for the first time in 1973, as mentioned above, at Beat ’72 in Rome. Perlini transposed the fragmentary nature typical of society at that time into what he defined as fragment-images, aimed at reducing the body of the actor on stage into sections of light and darkness, in the idiosyncratic drive to «parcel, shatter theatrical objects (fear of the body) but also a clear attempt to reconstruct them (desire to represent)»
Proteomic characterization of amyloid deposits in transthyretin amyloidosis associated with various mutations
No full textImaging of systemic amyloidosis
No full textIn systemic amyloidosis, several imaging techniques can be used to detect
the presence, extent, and localization of amyloid deposits, to monitor their progression
and regression, and to assess organ involvement and dysfunction. The presence
of heart involvement is the main prognostic determinant and most efforts have been
directed to the evaluation of cardiac amyloidosis. Heart involvement is classically
diagnosed based on increased ventricular wall thickness and myocardial echogenicity
(often referred to as “granular sparkling”) at echocardiography. However, more
refined echocardiographic techniques, such as myocardial integrated backscatter, tissue
Doppler, and strain imaging can provide evidence of early heart involvement and
add functional and prognostic information. Magnetic resonance imaging (MRI) and
cardiac scintigraphy with radiolabeled phosphate derivatives showed good sensitivity
and specificity in the detection of heart involvement. In particular, scintigraphy with
radiolabeled aprotinin can detect early amyloid deposits in the heart. Scintigraphy has
the advantage of specific tissue characterization. The prototype of a specific amyloid
tracer is iodinated serum amyloid P component (I-SAP). Scintigraphy with I-SAP is a
useful complement for the diagnosis and provides an estimation of amyloid load, and
serial studies can reveal disease progression and regression. However, I-SAP scintigraphy
cannot image the heart. Anatomo-functional imaging, via ultrasound, computed
tomography, and MRI scanning, is useful in the diagnosis and follow-up of localized
amyloidosis. Accurate imaging of amyloid deposits can now be combined with the
biochemical assessment of organ, particularly cardiac, damage and with reliable measurement
of the circulating precursors. This will improve the care of patients with
amyloidosis and shed light on the pathogenesis of organ damage
Effects of the chronic digoxin administration on extracellular matrix turnover in a model of cardiac hypertrophy in Guinea pigs.
No full textLobe-specific oxidative stress and matrix metalloproteinase activation in two animal models of non-alcoholic fatty liver disease (NAFLD)
No full textEffects of the chronic digoxin administration on extracellular matrix turn-over in a model of cardiac hypertrophy in guinea pig
No full textSynthesis of 2-amino-5-sulfanyl-1,3,4-thiadiazole derivatives and evaluation of their antidepressant and anxiolytic activity
No full textRecently a series of 2-amino-5-sulfanyl-1,3,4-thiadiazole derivatives bearing different substituents were synthesized and screened pharmacologically in order to evaluate their central nervous system activity. The purpose of this study was to evaluate the effects of the title compounds on CNS activity by varying the substituents in the thiadiazole moiety. It was found that some of these compounds possess marked antidepressant and anxiolytic properties comparable in efficiency to the reference drugs Imipramine and Diazepam. The most potent compound 3k was further investigated to complete its pharmacological profile with respect to undesired side effects. Behavioral results showed that 3k is a very promising compound, characterized by a mixed antidepressant-anxiolytic activity accompanied by a therapeutic dose range that is essentially 2 orders of magnitude less than that at which side effects such as sedation and amnesia are evident
Novel mitochondrial protein interactors of immunoglobulin light chains causing heart amyloidosis
No full textIn immunoglobulin (Ig) light-chain (LC) (AL) amyloidosis, AL deposition translates into life-threatening cardiomyopathy. Clinical and experimental evidence indicates that soluble cardiotoxic LCs are themselves harmful for cells, by which they are internalized. Hypothesizing that interaction of soluble cardiotoxic LCs with cellular proteins contributes to damage, we characterized their interactome in cardiac cells. LCs were purified from patients with AL amyloidosis cardiomyopathy or multiple myeloma without amyloidosis (the nonamyloidogenic/noncardiotoxic LCs served as controls) and employed at concentrations in the range observed in AL patients' sera. A functional proteomic approach, based on direct and inverse coimmunoprecipitation and mass spectrometry, allowed identifying LC-protein complexes. Findings were validated by colocalization, fluorescence lifetime imaging microscopy (FLIM)-fluorescence resonance energy transfer (FRET), and ultrastructural studies, using human primary cardiac fibroblasts (hCFs) and stem cell-derived cardiomyocytes. Amyloidogenic cardiotoxic LCs interact in vitro with specific intracellular proteins involved in viability and metabolism. Imaging confirmed that, especially in hCFs, cardiotoxic LCs (not controls) colocalize with mitochondria and spatially associate with selected interactors: mitochondrial optic atrophy 1-like protein and peroxisomal acyl-coenzyme A oxidase 1 (FLIM-FRET efficiencies 11 and 6%, respectively). Cardiotoxic LC-treated hCFs display mitochondrial ultrastructural changes, supporting mitochondrial involvement. We show that cardiotoxic LCs establish nonphysiologic protein-protein contacts in human cardiac cells, offering new clues on the pathogenesis of AL cardiomyopathy.-Lavatelli, F., Imperlini, E., Orrù, S., Rognoni, P., Sarnataro, D., Palladini, G., Malpasso, G., Soriano, M. E., Di Fonzo, A., Valentini, V., Gnecchi, M., Perlini, S., Salvatore, F., Merlini, G. Novel mitochondrial protein interactors of immunoglobulin light chains causing heart amyloidosis
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