1,721,101 research outputs found
Evaluation of matrix metalloproteinase-2 and -9 (gelatinase A and B) in the sera from patients with prostate diseases.
No full textAbstract
Background: It is widely recognized that the serum prostate-specific antigen (PSA) levels as a biomarker of prostate cancer is imperfect, in that it can have many false positive elevations attributable to benign hyperplasia and subclinical prostatic inflammation. There are increasing data that support a positive correlation between gelatinase (MMP-2 and-9) activity and tumor cell invasion and tumor aggressiveness.
Objectives: We evaluate gelatinolytic activities in the sera of patients with prostate carcinoma and benign prostate hyperplasia (BPH) in order to verify whether MMP-2 and-9 might have potential as non invasive biomarkers.
Methods: By gelatin zymography we verified MMP activity in a total of 43 patients. Of these 9 had BPH and 34 had cancer, consisting of 3 with Gleason score 5, 13 with Gleason 6, 14 with Gleason 7, 1 with Gleason 8, and 3 with Gleason 9.
Results: Four dominant proteinases were detected migrating at ~ 240, 130, 92 and 72 kDa. The most abundant lytic activity is at 92 kDa (MMP-9); whereas MMP-2 is present in lesser quantities. MMP-9 activity is enhanced in the sera from patients with cancer compared with BPH patients; whereas the 72 kDa band is similar in the two groups.
Conclusions: These results suggest that the inexpensive measurement of MMPs in serum may serve as a suitable supplementary tool to distinguish between patients with prostate cancer and patients with BPH, and the addition of this enzyme to currently available PSA and/or f-PSA/t-PSA ratio might provide clinicians additional objective information on prostate carcinomas
Role of gangliosides and phospholipids in thyrotropine-response of hyperplastic and neoplastic rat thyroid
No full textNuovi approcci in campo di diagnostica oncologica: la determinazione del recettore per l'epidermal growth factor (EGFr) nei tumori solidi umani
No full text
Desensitization and resensitization to TSH of thyroid gland from rats chronically treated with propylthiouracil
No full text
Analisi zimografica delle matrix metallo-proteinasi nei liquidi biologici di soggetti con patologie neoplastiche
No full textNuovi approcci in campo di diagnostica endocrinologica: il ruolo del laboratorio nella diagnosi delle basse ed alte stature
No full text- …
