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    Biotipizzazione ed antibioticoresistenza di ceppi di Escherichia coli isolati da conigli selvatici (Oryctolagus cuniculus) asintomatici

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    Biotyping and antibiotic resistance of Escherichia coli strains isolated from asymptomatic wild rabbits (Oryctolagus cuniculus). One hundred wild rabbits hunted in Serio Regional Park (Bergamo, Italy) were examinated. Post mortem examination revealed a good body condition, even in rabbits with ecto-endoparasites (Spilopsyllus cuniculi and Cittotaenia spp.). The caecum of each animal was tested bacteriologically for Escherichia coli. Sixty per cent of samples were positive. 25 strains were identified, biotyped and checked for antibiotic sensitivity (nalidixic acid, apramycin, chloramphenicol, chlortetracycline, ciprofloxacin, colistin, sulph.+trimethoprim, enrofloxacin, flumequine, gentamicin, neomycin, norfloxacin, streptomycin and the three sulphanilamides). Nine different biotypes were identified: B2 (n.1), B8 (4), B10 (3), B12 (4), B14 (5), B18 (1), B20 (2), B26 (1) and B30 (4). Antibiotic sensitivity varied widely: only norfloxacin was active against all the strains, chloramphenicol on 17, enrofloxacin only on 12, and chlortetracycline and the sulphanilamides were virtually inactive. The presence of different pathogenic biotypes of E. coli and their multi-resistance to antibiotics has never been reported in wild rabbits. These preliminary findings need to be completed with serotype studies and identification of the gene coding for intimin and adesines, to give a fuller picture of the situation in these wild animals

    Preliminary researches to standardize a method of quantitative analysis on Lactobacillus acidophilus in poultry feed

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    The study focuses on the method and the problems about quantitative analyses in the research on Lactobacillus acidophilus after its addition to commercial poultry-feed, whose rough grinding is not suitable for the "IDF Standard quantitative method for lactic acid bacteria count at 37°" employed in dairy products. Poultry-feed was prepared every month. A sample was collected before and after adding Lactobacillus acidophilus, while analyses were carried out respectively at T 0, 15, and 28 days after the food storage at 4-6°C. The best outcome (more 30% of recovered cells compared to the standard method) resulted from samples subjected to the homogenization and the addition of Skim Milk Powder

    Lesioni podali e benessere del broiler: osservazioni preliminari

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    Foot pad dermatitis and welfare in broiler chickens. An attempt to classify broiler foot health status using the method developed in Sweden by Ekstrand et al. for the control of foot pad dermatitis was carried out. The Italian market demanded heavy broilers (3.5 kg and more) at 56 days of age. In this condition, the foot pad dermatitis was frequent as a consequence of feed composition, litter material, stocking density and climatic conditions. Foot pad dermatitis evaluation and classification of lesions at slaughterhouses were very difficult. Gross and histological lesions were not always comparabl

    Valutazioni preliminari di una miscela di Lactobacillus acidofilus e Lactobacillus plantarum sulle performance della gallina ovaiola e sulla qualità dell'uovo

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    Lactobacilli are commonly used as probiotics in poultry production for their beneficial effect on the host through maintenance of the gut microbiota balance. The aim of this study was to evaluate the effect of a mixture of L.acidophilus and L. plantarum administered with drinking water on egg quality and layer performance. The trial, performed on two different farms, was conducted on 480 laying hens which were housed in pens. The animals, from 21 to 44 weeks of age were divided into two groups: the hens of the Treatment group (T) received water with the supplementation of 100000000 CFU/animal/day of the experimental mixture, while animals of the other group received water without any supplementation (Control =C). Number and weight of eggs were recorded daily, while feed consumption and body weights of the animals were registered every week and monthly, respectively. At 25, 30, 35, 40 and 44 weeks of age, specific gravity of eggs, albumen quality (Haugh Units) and shell thickness were measured on 30 eggs/group. During the experimental period, body weight, daily feed intake and feed conversion rate of the animals were not significantly affected by the supplementation of the microbial feed additive. Moreover, no significant difference was observed in egg production and egg mass of hens between the two groups. Egg quality, instead, was influenced by the experimental treatment; in particular, specific gravity and Haugh Units of eggs in the T group were significantly higher in comparison to the C group. In conclusion, the supplementation of this mixture in drinking water did not influence growth performance of hens, but seemed to improve some characteristic of egg quality
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