191 research outputs found
Robert Barraco, MD, LVHN Chief Academic Officer
No full textWhat is the role of a Chief Academic Officer? Robert Barraco, MD, the physician who holds that position at LVHN explains and shares details about SELECT, a medical education program created by the University of South Florida College of Medicine and LVHN.https://scholarlyworks.lvhn.org/essential-partners-podcast/1005/thumbnail.jp
Il ruolo delle connessioni geometriche nell'osteointegrazione implantare: platform switching
No full textDurante gli ultimi anni sono state proposte diverse soluzioni di connessioni implantari al fine di ottenere un accettabile livello di osteointegrazione. Lo scopo di questo lavoro è stato quello di valutare il riassorbimento dell'osso crestale periimplantare, dopo inserimenti di impianti a connessione avvitata e a connessione cronometrica con platform switching. Abbiamo osservato come nei casi trattati con connessioni interne e platform switching si ottiene un mantenimento significativo dei livelli di osso crestale, mentre nei casi trattati con sistematica avvitata si è rilevato un riassorbimento fino almeno alla prima spira dell'impianto. E' per questo importante ampliare il numero di casi clinici da osservare
Complete and long-lasting cytologic and molecular remission of FIP1L1-PDGFRA-positive acute eosinophil myeloid leukaemia, treated with low-dose imatinib monotherapy.
No full textMyeloproliferative neoplasms associated with FIP1L1-PDGFR rearrangements represent a rare subset of myeloid and lymphoid malignancies, characterised by the presence of eosinophilia and abnormalities of PDGFRA, PDGFRB or FGFR1 genes. The fusion product of such genes is a tyrosine kinase oncoprotein sensitive to imatinib, which to date results to be the standard of care for FIP1L1-PDGFRA-positive chronic myeloproliferative disorders with eosinophilia. However, the coexistence of FIP1L1-PDGFRA rearrangement associated with acute myeloid leukaemia is extremely rare. Here, we report a rare case of FIP1L1-PDGFRA-positive acute myeloid leukaemia, with marked peripheral blood and bone marrow eosinophilia, treated with low dose of imatinib monotherapy, achieving a rapid and long-lasting complete cytologic and molecular remission, without need for intensive chemotherapy
Investigating the cryopreservation of nodal explants of Lithodora rosmarinifolia (Ten.) Johnst., a rare, endemic Mediterranean species
No full textIn this study, we investigated the possibility of using the droplet-vitrification technique for cryopreserving nodal segments of in vitro plantlets of the endangered plant species Lithodora rosmarinifolia. Among the three vitrification solutions tested, only solutions B1, containing (w/v) 50 % glycerol and 50 % sucrose, and B3, containing 40 % glycerol and 40 % sucrose, were able to induce cryotolerance in nodal explants, resulting in intermediate survival and recovery after cryopreservation. A three-step vitrification protocol, including an additional dehydration treatment with half-strength vitrification solution for 30 min before the treatment with full-strength vitrification solution, did not lead to any improvement in survival and recovery compared with the two-step protocol. The optimal protocol was the following: preculture of nodal segments in liquid medium with 0.3 M sucrose for 16 h and 0.7 M sucrose for 5 h, treatment for 20 min in loading solution containing 1.9 M glycerol + 0.5 M sucrose, dehydration with vitrification solution B1 (glycerol 50.0 %, sucrose 50.0 %, w/v) for 60 min at room temperature, rapid cooling in minute droplets of vitrification solution, and rapid rewarming by immersion of nodal segments for 20 min in unloading solution containing 1.2 M sucrose. Under these conditions, 33 % recovery of cryopreserved nodal explants was achieved. Regrowth of cryopreserved samples was rapid and direct. These results indicate that long-term storage of L. rosmarinifolia by means of cryopreservation of nodal segments is possible, thereby contributing to securing the diversity of this rare and endangered plant species
Non-coding RNAs functioning in colorectal cancer stem cells
No full textIn recent years, the hypothesis of the presence of tumor-initiating cancer stem cells (CSCs) has received a considerable support. This model suggested the existence of CSCs which, thanks to their self-renewal properties, are able to drive the expansion and the maintenance of malignant cell populations with invasive and metastatic potential in cancer. Increasing evidence showed the ability of such cells to acquire self-renewal, multipotency, angiogenic potential, immune evasion, symmetrical and asymmetrical divisions which, along with the presence of several DNA repair mechanisms, further enhance their oncogenic potential making them highly resistant to common anticancer treatments. The main signaling pathways involved in the homeostasis of colorectal (CRC) stem cells are the Wnt, Notch, Sonic Hedgehog, and Bone Morfogenic Protein (BMP) pathways, which are mostly responsible for all the features that have been widely referred to stem cells. The same pathways have been identifi ed in colorectal cancer stem cells (CRCSCs), conferring a more aggressive phenotype compared to non-stem CRC cells. Recently, several evidences suggested that non-coding RNAs (ncRNAs) may play a crucial role in the regulation of different biological mechanisms in CRC, by modulating the expression of critical stem cell transcription factors that have been found active in CSCs. In this chapter, we will discuss the involvement of ncRNAs, especially microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), in stemness acquisition and maintenance by CRCSCs, through the regulation of pathways modulating the CSC phenotype and growth, carcinogenesis, differentiation, and epithelial to mesenchymal transition (EMT)
Dynamic identification of helicopter structures using operational modal analysis methods in the presence of harmonic loading
No full textThis paper reports the activities performed to evaluate the effectiveness of a number of selected Operational Modal Analysis methodologies, based on both frequency- and time-domain algorithm, dealing with a class of input excitation composed by a broad band white noise corrupted with harmonic loadings, characterizing the dynamic environment of helicopters. The main objective is to evaluate how this class of excitation (that differs from the pure random noise excitation required by all the Operational Modal Analysis methods) affects the estimate of the modal parameters. Moreover, the sensitivity of the proposed approaches to the harmonic loading to the random energy ratio is investigated. An actual medium class helicopter structure, a time expired Lynx Mk7 airframe located at the University of Bristol laboratory facilities, and an AgustaWestland lightweight helicopter tail are considered for this purpose. The modal parameters of such structures are estimated in the Operational Modal Analysis framework only considering the vibration responses due to several multiple stochastic random and sinusoidal excitations, provided by electro-dynamic shakers. Such estimates are compared against those derived from the more traditional input/output-based Experimental Modal Analysis approach, which is used as a reference to assess the performance of the considered Operational Modal Analysis methods
Noise reduction for magnetic resonance imaging by wavelet. An application to the study of the capilary water absorption in sedimentary rocks
No full textApplicazione di trasformate wavelet per la riduzione del rumore nelle immagini NMR di rocce sedimentarie
No full textIl sempre crescente interesse verso i Beni Culturali suscita molteplici problematiche inerenti la salvaguardia e la diagnosi del loro stato di conservazione. In questo ambito, lo studio delle caratteristiche, quali ad esempio le proprietà capillari delle rocce sedimentarie, ricopre un’importanza cruciale non solo per studiare lo stato di conservazione ma anche per fornire utili indicazioni sulle possibili tecniche e sui materiali da utilizzare negli eventuali trattamenti di protezione e restauro.
Al fine di ottenere approfondite informazioni sulle proprietà capillari, il presente lavoro propone un metodo matematico utile nello studio della cinetica di assorbimento capillare. Lo studio è stato condotto su una roccia sedimentaria particolarmente omogenea, la Pietra di Lecce, analizzando le immagini del campione, acquisite mediante la tecnica di imaging NMR (Nuclear Magnetic Resonance, MRI). Il rumore presente nelle immagini è stato ridotto grazie all’utilizzo delle trasformate wavelet. Le immagini elaborate e ricostruite tramite un algoritmo che implementa la trasformata DWT (Discrete Wavelet Transform) – epsilon-decimata, forniscono utili informazioni per la caratterizzazione della cinetica di assorbimento dell’acqua
Are Long Noncoding RNAs New Potential Biomarkers in Gastrointestinal Stromal Tumors (GISTs)? The Role of H19 and MALAT1
No full textLong noncoding RNAs (lncRNAs) are emerging as key regulators of genetic and epigenetic networks, and their deregulation may underlie complex diseases, such as carcinogenesis. Several studies described lncRNA alterations in patients with solid tumors. In particular, HOTAIR upregulation has been associated with tumor aggressiveness, metastasis, and poor survival in gastrointestinal stromal tumor (GIST) patients. We analyzed expression levels of other lncRNAs, H19 and MALAT1, in FFPE tissue specimens from 40 surgically resected and metastatic GIST patients, using real-time PCR analysis. H19 and MALAT1 were both upregulated in 50% of GIST patients. MALAT1 lncRNA expression levels seem to be correlated with c-KIT mutation status. The percentage of both H19 and MALAT1 upregulation was significantly higher in patients with time to progression (TTP) < 6 months as compared to patients with TTP > 6 months. The median TTP was significantly lower in patients with both H19 and MALAT1 lncRNA upregulation as compared to those with lncRNA downregulation. These data suggest a potential role for both H19 and MALAT1 lncRNAs as prognostic biomarker for the clinical selection of the best candidate to first-line treatment with imatinib
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