6,950 research outputs found
TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation
Get PDFThe interaction between TACC3 (transforming acidic coiled coil protein 3) and the microtubule polymerase ch-TOG (colonic, hepatic tumor overexpressed gene) is evolutionarily conserved. Loading of TACC3–ch-TOG onto spindle microtubules requires the phosphorylation of TACC3 by Aurora-A kinase and the subsequent interaction of TACC3 with clathrin to form a microtubule binding surface. Whether there is a pool of TACC3–ch-TOG that is independent of clathrin in human cells, and what is the function of this pool, are open questions. Here, we report that TACC3 is recruited to the plus-ends of microtubules by its association with ch-TOG and that this pool is independent of phosphorylation and binding to clathrin. The plus-end binding of TACC3–ch-TOG persists in interphase and we propose that one cellular function of TACC3–ch-TOG is to modulate cell migration. We also describe the distinct subcellular pools of TACC3, ch-TOG and clathrin. TACC3 is often described as a centrosomal protein, but we show that there is no significant population of TACC3 at centrosomes. The delineation of distinct protein pools reveals a simplified view of how these proteins are organized and controlled by post-translational modification
Analysis of morphometry and mitochondrial DNA Sequences from two Trichiurus species in waters of the western North Pacific: Taxonomic assessment and population structure
No full textMicrosatellite DNA markers for population-genetic studies of blue mackerel (Scomber australasicus) and cross-specific amplication in S. japonicus
No full textChimeric polypeptide of thymidine kinase and thymidylate kinase of shrimp white spot syndrome virus: thymidine kinase activity of the recombinant protein expressed in a baculovirus/insect cell system.
No full textLack of TEL-AML1 fusion transcript resulting from a cryptic t (12;21) in adult B lineage acute lymphoblastic leukemia in Taiwan.
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Synthesis and Crystal Structure of [CuI(N-Ch-Bqdi)2]Cl (N-Ch-Bqdi = N- Cyclohexyl-Bezoquinodiimine). a Novel D10 Benzoquinonediimine Complex
No full textSimultaneous determination of adenosine and its metabolites by capillary electrophoresis as a rapid monitoring tool for 5 '-nucleotidase activity
No full text[[abstract]]A simple and rapid capillary electrophoretic method was developed for the simultaneous determination of micro-molar adenosine, hypoxanthine and inosine in enzyme assays without using radioactive labeled substrates. Prior to electrophoretic separation, addition of acetonitrile and sodium chloride to the assay solution and brief centrifugation are recommended for the purpose of sample cleanup and sample stacking. Under the optimal condition, the good separation with high efficiency was achieved in 6 min. Using deoxyadenylate as an internal standard, the linear range of the method was 5-200 mu M, and the concentration limits of detection of adenosine, hypoxanthine and inosine were 2.2, 3.6 and 1.4 mu M, respectively. Application of the proposed method was demonstrated by the activity assay of 5'-nucleotidase from Hep G2 cells. (c) 2006 Elsevier B.V. All fights reserved.[[note]]SC
DNA-gold nanorod conjugates for remote control of localized gene expression by near infrared irradiation.
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