1,539 research outputs found

    Immunolocalization of the mercurial-insensitive water channel and glycerol intrinsic protein in epithelial cell plasma membranes

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    Two water channel homologs were cloned recently from rat kidney, mercurial-insensitive water channel (MIWC) and glycerol intrinsic protein (GLIP). Polyclonal antibodies were raised against synthetic C-terminal peptides and purified by affinity chromatography. MIWC and CLIP antibodies recognized proteins in rat kidney with an apparent molecular mass of 30 and 27 kDa, respectively, and did not cross-react, By immunofluorescence, MIWC and GLIP were expressed together on the basolateral plasma membrane of collecting duct principal cells in kidney, By immunohistochemistry, MIWC and GLIP were expressed on tracheal epithelial cells with greater expression of GLIP on the basal plasma membrane and Mn;VC on the lateral membrane; only MIWC was expressed in bronchial epithelia, In eye, GLIP was expressed in conjunctival epithelium, whereas MIWC was found in iris, ciliary body, and neural cell layers in retina, MIWC and GLIP colocalized on the basolateral membrane of villus epithelial cells in colon and brain ependymal cells, Expression of MIWC and GLIP was not detected in small intestine, liver, spleen, endothelia, and cells that express water channels CHIP28 or WCH-CD, These studies suggest water/solute transporting roles for MIWC and GLIP in the urinary concentrating mechanism, cerebrospinal fluid absorption, ocular fluid balance, fecal dehydration, and airway humidification. The unexpected membrane colocalization of MIWC and GLIP in several tissues suggests an interaction at the molecular and/or functional levels

    Cw ion lasers pumped by electron beams

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    We have obtained cw laser radiation from singly ionized Hg, I, Cd, Se, and As by exciting He metal-vapor mixtures with a dc electron beam. The beam is generated by glow discharge electron guns located apart from the active medium. This is the first time that cw ion laser action has been obtained using electron beam excitation

    CW laser action in atomic fluorine

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    We have obtained CW laser action on four transitions in the doublet system of atomic fluorine for the first time. All previously reported laser action was on a pulsed basis only. CW laser radiation was obtained when F2 or AgF was used as a fluorine donor in an electron beam pumped helium plasma. A multiline output power of 200 mW was obtained

    CW silver ion laser with electron beam excitation

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    A CW laser power of 140 mW was obtained in the 840.39 nm transition of Ag II by electron beam excitation. This electron beam excited metal vapor ion laser is capable of operating using metals with high vaporization temperatures and is of interest for generation of CW coherent radiation in the 220-260 nm spectral region

    1-W cw Zn ion laser

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    We have obtained 1.2W of cw laser power on the 4911.6- and 4924.0-Å transitions of Zn II by exciting a He-Zn gas mixture with a dc glow discharge electron beam. In addition, 0.25-W output power has been obtained on the 6149.9-Å line of Hg+ using the same excitation scheme. The combination of electron beam ionization of rare gas atoms and subsequent charge transfer excitation to metal ion levels is shown to have the potential of significantly increasing the efficiency of ion lasers. cw multiwatt visible and ultraviolet ion lasers operating at efficiencies > 10-3 appear feasible using this excitation scheme

    Stabilization as a CW approximation

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    AbstractThis paper describes a peculiar property of the category of S-modules constructed by the author, Kriz, Mandell, and May: the full subcategory of suspension spectra (all of which are S-modules) forms a precise copy of the category of topological spaces. Consequently, the “classical” homotopy category of S-modules with morphisms the actual homotopy classes of maps contains a copy of unstable homotopy theory. Stabilization and stable homotopy are induced by CW approximation as S-modules. One consequence is that CW complexes whose suspension spectra are CW S-modules must be contractible

    Cw iodine ion laser excited by an electron beam

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    CW laser oscillation has been obtained on the 5760.7 and6127.5 Å transitions of singly ionized iodine in an He-I2 plasma excited by a dc electron beam

    A closed manifold is a fat CW complex

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    In this paper, we introduce a notion of a fat CW complex to show that a closed manifold is a regular CW complex, while it is not always the case if we discuss about a smooth CW structure, introduced by the first author, instead of a fat CW structure. We also verify that de Rham theorem holds for a fat CW complex and that a regular CW complex is reflexive in the sense of Y.~Karshon, J.~Watts and P.~I-Zemmour. Further, any topological CW complex is topologically homotopy equivalent to a fat CW complex. It implies that there are lots of non-manifold examples supporting de Rham theorem.Comment: 17 page

    Electron beam pumped cw Se II laser

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    We have observed cw laser action in 15 transitions of singly ionized selenium using an electron beam to excite a He-Se mixture. The variation of the laser output power as a function of the electron beam discharge parameters is reported

    Cw laser oscillations in Cd II in an electron beam created plasma

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    A cw Cd II laser has been demonstrated using electron beam excitation. Cw laser oscillation has been obtained on six transitions of Cd 11 in a He-Cd plasma. Output power as a function of electron beam discharge parameters has been investigated
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