1,720,990 research outputs found
Su alcune patologie riscontrate in ostriche piatte (Ostrea edulis) provenienti da un banco naturale
No full textUlteriori dati sulle patologie infettive e infestive dei molluschi eduli lamellibranchi allevati o raccolti sui litorali pugliesi
No full textRicerche sulla presenza di virus influenzali e relativi anticorpi in cavalli importati dall'estero a scopo di macellazione
No full textDati preliminari sulla patologia infettiva ed infestiva dei Molluschi eduli Lamellibranchi allevati o raccolti sui litorali Pugliesi
No full textEvaluation of the pathogenicity to pregnant rabbits of a bovine Herpesvirus-4 (BHV-4) strain
No full text
The endocannabinoid system in the mediterranean mussel mytilus galloprovincialis: Possible mediators of the immune activity?
Full text linkAnandamide (AEA) is one of the best characterized members of the endocannabinoid family and its involvement in many pathophysiological processes has been well documented in vertebrates and invertebrates. Here, we report the biochemical and functional characterization of key elements of the endocannabinoid system in hemocytes isolated from the Mediterranean mussel Mytilus galloprovincialis. We also show the effects of exogenous AEA, as well as of capsaicin, on the cell ability to migrate and to activate the respiratory burst, upon in vitro stimulation of phagocytosis. Interestingly, our findings show that both AEA and capsaicin suppress the hemocyte response and that the use of selective antagonists of CB2 and TRPV1 receptors revert their inhibitory effects. Overall, present data support previous evidence on the presence of endocannabinoid signaling in mollusks and advance our knowledge about the evolutionary origins of this endogenous system and its role in the innate response of mollusks
L'immunodiffusione in gel di agar (AGIDT) per la diagnosi dell'infezione da virus sinciziale felino. (FeSFV)
No full textExperimental vaccine trial against vibriosis in Dicentrarchus labrax (L.).
No full textImmunohistochemistry (IHC) showed first appearance of Ig+ cells in the head kidney (HK) of 50 days post-hatch (dph) sea bass larvae. Rare Ig+ cells were also found in spleen and thymus. Till 70 dph these became numerous in the HK (70±9 cells/section) and spleen (34±6), less frequent in the thymus (12±4) and absent in the GI tract. RT-PCR with specific primers for the constant region of sea bass Ig H chain and β-actin (housekeeping gene) confirmed Ig transcripts at 50 dph and their rise till 70 dph.
Therefore, a vaccine trial vs. Vibrio anguillarum was established using AQUAVAC VIBRIO VAB (biotypes I-II, Shering Plough). Fry were immersion vaccinated (1:10, 1-min) at 95 dph, immersion boosted (same dose) at 165 dph and i.p. boosted (0.1 ml) at 233 dph. Final samplings were made at 291 dph.
Bacterial agglutination (BA) by sera and quantitative IHC showed significantly higher BA activity and numbers of mAb DLIg3+ cells in the HK of fish immersion boosted (233 dph) than in age-matched untreated controls and immersion vaccinated only (165 dph). The higher BA lasted till 291 dph, without any further enhancement by i.p. boost, whereas more numerous Ig+ cells were in the HK and spleen of i.p. boosted group at 291 dph.
Experimental challenges against biotype I Vibrio anguillarum 77/I03 strain (2.4-6.2 million CFU/ml, 200 ul i.p.) of fish treated as above showed significant protective effect exerted by immersion boost, and almost complete protection (survival) following further i.p. boost
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