540 research outputs found

    Patti Smith and Andi Ostrowe in a hotel room, Washington, D.C., July 1978

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    Musician, poet, and author Patti Smith poses holding a clarinet in a hotel room in July 1978. Andi Ostrowe, working as Smith's personal assistant, reclines on the bed in the background.The photograph was taken by Don Hamerman as part of a session for "Unicorn Times," an alternative performing arts periodical in Washington, D.C. The photograph appeared in the September 1978 issue of "Unicorn Times.

    Patti Smith and Andi Ostrowe being photographed by Don Hamerman, Washington, D.C., July 1978

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    Musician, poet, and author Patti Smith poses as Don Hamerman photographs her and Andi Ostrowe in a Washington, D.C. hotel room in July 1978. Smith was in Washington to perform at the Warner Theatre and Ostrowe was then working as Smith's personal assistant. The photograph was taken by Don Hamerman, who was then a staff photographer for Unicorn Times, a monthly performing arts periodical in Washington, D.C

    Patti Smith poses as Andi Ostrowe reclines, Washington, D.C., circa July 1978

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    Musician, poet, and author Patti Smith posing as Andi Ostrowe smokes on a bed in a Washington, D.C. hotel room in July 1978. Smith was in Washington to perform at the Warner Theatre and Ostrowe was then working as Smith's personal assistant. The photograph was taken by Don Hamerman, who was then a staff photographer for Unicorn Times, a monthly performing arts periodical in Washington, D.C

    Inszenierte Dokumente: zu Paul Bowles' Sammlung traditioneller marokkanischer Musik

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    Andi Schoon widmet sich in diesem Beitrag der Frage nach dokumentarischer Authentizität: Der amerikanische Schriftsteller Paul Bowles (1910-1999) reiste, kurz nachdem Marokko 1956 unabhängig geworden war, monatelang durch das Land, um traditionelle Musikstile wie Ahwouach, Andaluz, Gnawa und Rwais zu dokumentieren. Der Beitrag beleuchtet das Zustandekommen dieser Aufnahmen

    ANALISIS RASIO RNA/DNA UDANG WINDU Penaeus monodon HASIL SELEKSI TUMBUH CEPAT Andi

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    Seleksi udang windu Penaeus monodon tumbuh cepat menggunakan marker DNA telah berhasil dilakukan dalam upaya perakitan strain unggul udang windu. Udang windu hasil seleksi menunjukkan adanya peningkatan pertumbuhan dibandingkan dengan tanpa seleksi (kontrol). Rasio RNA/DNA merupakan salah satu parameter yang telah banyak digunakan dalam menentukan kualitas ikan/udang di antaranya adalah pertumbuhan. Penelitian ini bertujuan untuk mengetahui rasio RNA/DNA pada udang windu hasil seleksi tumbuh cepat dan kontrol (tanpa seleksi). Sampel udang windu tumbuh cepat yang digunakan berukuran bobot 50,66±16,51 g dan panjang 17,55±1,93 cm; sedangkan udang kontrol berukuran bobot 29,64±11,93 g dan panjang 14,78±2,53 cm. Metode isolasi total RNA dilakukan dengan menggunakan kit isogen, sedangkan genom DNA menggunakan metode konvensional fenol kloroform. Konsentrasi RNA dan DNA hasil isolasi diukur menggunakan GeneQuant. T-test dari Statistix Versi 3,0 digunakan untuk membedakan rasio RNA/DNA antara kedua perlakuan yang dianalisis. Hasil penelitian menunjukkan bahwa genom DNA dan total RNA mempunyai tingkat kemurnian yang tinggi. Hasil analisis t-test menunjukkanbahwa rasio RNA/DNA udang windu tumbuh cepat (4,51) berbeda secara nyata (P<0,05) dengan udang windu kontrol (3,19). Kecenderungan rasio RNA/DNA semakin tinggi dengan semakin beratnya bobot badan, di mana rasio RNA/DNA udang betina (4,96) lebih tinggi (P<0,05) dari udang jantan (2,93). Analisis regresi menunjukkan bahwa rasio RNA/DNA udang windu memiliki hubungan erat dengan panjang (R=0,5628) dan bobot (R=0,6539). Hasil penelitian ini berimplikasi bahwa parameter rasio RNA/DNA dapat dijadikan sebagai indikator pertumbuhan udang windu

    Patti Smith and Don Hamerman, Washington, D.C., July 1978

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    Musician, poet, and author Patti Smith poses in a hotel room mirror in July 1978. The photograph was taken as part of a session for "Unicorn Times," an alternative performing arts periodical in Washington, D.C. Photographer Don Hamerman is visible in the picture, as is Smith's personal assistant Andi Ostrowe, who lies on the bed

    GENETIC VARIABILITY AND POPULATION STRUCTURE OF GROUPER (Epinephelus suillus) FROM MAKASSAR STRAIT AND BONE BAY, SOUTH SULAWESI, INDONESIA

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    Random Amplified Polymorphic DNA (RAPD) was employed to determine the genetic variability and population structure of grouper (Epinephelus suillus) from Makassar Strait and Bone Bay, South Sulawesi, Indonesia. Genomic DNA was isolated from preserved muscle tissue using Phenol-Chloroform technique. Among 24-screened arbitrary primers, ten primers (OPA-02, OPA-06, OPA-08, OPA-10, OPA-15, OPA-16, OPA-17, OPA-18, OPA-19 and CA-05) were selected to generate RAPD fingerprinting of grouper populations. The ten primers generated a total of 212 fragments (loci) and 120 polymorphic fragments in their size ranging from 250 to 2,500bp. The high polymorphism (60%) was obtained from Makassar population followed by Bone (59%) and Pare-Pare populations (50%). Similarity index of individuals was 0.86±0.07 for Pare-Pare, 0.80±0.11 for Makassar and 0.82±0.07 for Bone population. Fifteen fragments from ten primes were identified as species-specific markers of E. suillus. The UPGMA cluster analysis showed that the dendrogram seemed to be clustered according to their geographical location, where Pare-Pare population was genetically closer to Makassar population (D=0.20) than to Bone population (D=0.24)

    OVERCOMING CHALLENGES IN THE AIRPORT AUTHORITY OF ANDI JEMMA MASAMBA AIRPORT

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    Airport management always poses challenges in its administration. In addition to requiring high-security standards, airport management must provide well-standardized services. This research was conducted to understand how a well-planned airport management development aligns with existing standards. In this study, the author tested the hypothesis regarding the importance of commanding/charismatic leadership in ensuring the implementation of an airport management system that meets service standards. The research was conducted at Andi Jemma Masamba Airport in Sulawesi, and the results showed a strong correlation between commanding/charismatic leadership and the improvement of quality at Andi Jemma Masamba Airport

    Implementación de un manual de funciones específicas del pasante en la Cámara de la Industria Automotriz de la ANDI

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    En el presente documento se exponen los argumentos, el desarrollo y resultados esperados del proceso de implementación del plan de mejora o manual de funciones específicas para pasante. Esto, tras haber realizado la práctica profesional dentro de la Cámara de la Industria Automotriz de la Asociación Nacional de Empresarios de Colombia, de ahora en adelante, ANDI. Principal agremiación representante del sector privado de Colombia y reconocida por el Ministerio de Comercio, Industria y Turismo (MinCIT, 2021). Inicialmente se evidencian los aspectos generales de la ANDI, tales como: Misión, Visión, Valores, ubicación geográfica y estructura organizacional. De esta manera, al brindar una conceptualización de las funciones y composición de esta agremiación, en general, se facilita el entendimiento de los beneficios de la aplicación del proyecto dentro de la Cámara de la Industria Automotriz. Sustentando la manera en cómo la contribución al mejoramiento del desempeño del pasante, facilita el aprovechamiento de la visión fresca, disruptiva e intrépida, por la que se caracteriza el talento joven (Confama, 2021), fructuoso en procesos de constante evolución. Además, se expone el procedimiento del proyecto, por medio del planteamiento del objetivo general y los objetivos específicos. Finalmente, se exponen las conclusiones y resultados que traería una primera aplicación de proyecto, junto con una sección de seguimiento de práctica profesional, donde se destacan los logros y experiencia obtenida por el autor durante su pasantía en la Cámara de la Industria Automotriz de la ANDI.This document presents the arguments, development and expected results of the implementation process of the improvement plan or manual of specific functions for interns. This, after having carried out the professional practice within the Chamber of the Automotive Industry of the National Association of Entrepreneurs of Colombia, from now on, ANDI. Main association representing the private sector in Colombia and recognized by the Ministry of Commerce, Industry and Tourism (MinCIT, 2021). Initially, the general aspects of ANDI are evidenced, such as: Mission, Vision, Values, geographic location and organizational structure. In this way, by providing a conceptualization of the functions and composition of this association, in general, the understanding of the benefits of the application of the project within the Chamber of the Automotive Industry is facilitated. Supporting the way in which the contribution to the improvement of the intern's performance, facilitates the use of the fresh, disruptive and intrepid vision, by which young talent is characterized (Confama, 2021), fruitful in processes of constant evolution. In addition, the procedure of the project is exposed, through the approach of the general objective and the specific objectives. Finally, the conclusions and results that a first project application would bring are presented, along with a professional practice follow-up section, where the achievements and experience obtained by the author during his internship at the ANDI Chamber of the Automotive Industry are highlighted.Profesional en Negocios Internacionaleshttp://unidadinvestigacion.usta.edu.coPregrad

    CHARACTERISTICS OF VIRAL PROTEIN, VP-15, OF WHITESPOT SYNDROME VIRUS ISOLATED FROM INFECTED TIGER SHRIMP Penaeus monodon (Fabricius, 1798)

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    White spot syndrome virus (WSSV) has caused mass mortality on tiger shrimp (Penaeus monodon) culture and adversely affects prawn industry worldwide including Indonesia. It is well known that the protein structure of WSSV plays an important role in the virus infection and morphogenesis process. A viral protein structure called VP-15 is located in the nucleocapsid of virion virus. The protein structure involves in the life cycle of WSSV in host cells. A gene encoding VP-15 could be involved in constructing the RNA interference (RNAi), so it is needed to isolate and characterize for RNAi technology purpose. The study was aimed to isolate and characterize the VP-15 from the infected WSSV tiger shrimp. The characterization of VP-15 was undertaken through assessment of nucleotide sequence, amino acid deduction, alignment nucleotide/protein searches using Genetyx and BLAST program, and dendrogram construction analysis. The results showed that VP-15 was successfully isolated in form of ORFDNA with a fragment size of 243 bp. The phylogenetic tree analysis revealed three clusters corresponding to the time (year) of isolates collection. The VP-15 consisted of 80 amino acids, two start codons (ATG), one stop codon (TAA), and one Kozak context (AAAATGG). Hydrophilic amino acid was the highest composition (44.2%), followed by neutral (31.2%) and hydrophobic (24.6%) amino acid groups. The VP-15 was rich in amino acid of lysine (21.3%), arginine (22.9%) and serine (24.6%). The successful isolation of VP-15 is a very important step in providing a basic yet suitable material in constructing the dsRNA vaccine to control shrimp diseases in aquaculture.</jats:p
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