1,720,969 research outputs found
Pedobacter panaciterrae sp nov., isolated from soil in South Korea
No full textA Gram-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore-forming bacterial strain, designated Gsoil 042(T), was isolated from a soil sample of a ginseng field in Pocheon province (South Korea) and was characterized taxonomically by using a polyphasic approach. The isolate was positive for catalase and oxidase, but negative for gelatinase and production of indole and H2S. The predominant isoprenoid quinone was menaquinone 7 (MK-7). The major cellular fatty acids were summed feature 4 (C-16:1 omega 7c and/or iso-C-15:0 2-OH; 35.7%), iso-C-15:0 (24.3%) and iso-C-17:0 3-OH (11.6%). Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 042(T) fell within the radiation of the cluster comprising Pedobacter species. On the basis of 16S rRNA gene sequence similarities, the phylogenetically closest recognized species of the genus Pedobacter to strain Gsoil 042(T) included Pedobacter heparinus DSM 2366(T) (98.1%) Pedobacter africanus DSM 12126(T) (97.8%)and Pedobacter caeni LMG 22862(T) (97.2%) The 16S rRNA gene sequence similarity with other recognized species of the genus Pedobacter was less than 96.1%. DNA-DNA hybridization experiments showed that the DNA-DNA relatedness between strain Gsoil 042(T) and its phylogenetically closest neighbours was less than 43%. On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 042(T) should be classified as representing a novel species in the genus Pedobacter, for which the name Pedobacter panaciterrae sp. nov. is proposed. The type strain is Gsoil 042(T) (= KCTC 12594(T) = LMG 23400(T))
Methylibium fulvum sp nov., a member of the Betaproteobacteria isolated from ginseng field soil, and emended description of the genus Methylibium
No full textTwo bacterial strains, designated Gsoil 322 T and Gsoil 328, were isolated from soil of a ginseng field in Pocheon province (Republic of Korea). The strains were Gram-negative, motile, aerobic rods that showed nearly identical physiological profiles and similar chemotaxonomic profiles. The two strains were oxidase-positive but catalase-negative, reduced nitrate to nitrite and had fatty acid profiles in which C-16:0, C-17:0 cyclo and C-16:10 omega 7c/iso-C-15:0 2-OH were predominant. The DNA G + C contents of Gsoil 322(T) and Gsoil 328 were 66.6 and 66.7 mol%, respectively. Q-8 was observed as the major quinone. Comparative 16S rRNA gene sequence analysis showed that strain Gsoil 322 T belongs to the class Betaproteobacteria and was most closely related to Methylibium petroleiphilum ATCC BAA-1232(T) (97.5% sequence similarity). On the basis of its phenotypic properties and phylogenetic distinctiveness, strain Gsoil 322(T) (=KCTC 12591(T) =LMG 23394(T)) was classified in the genus Methylibium as the type strain of a novel species, for which the name Methylibium fulvum sp. nov. is proposed
Phenylobacterium koreense sp nov., isolated from South Korea
No full textT A Gram-negative, aerobic, rod-shaped, non-spore-forming bacterium, Slu-01(T) , was isolated from activated sludge from a wastewater treatment plant in Daejeon, South Korea. After 3 days on R2A medium, strain Slu-01(T) developed colourless colonies (0(.)-7-1(.)2 mm). The non-motile rods (0(.)7-1(.)0 x 1(.)0-2(.)0 mu m) were slightly curved and occurred singly and in pairs. No filamentous cells were found. On the basis of 16S rRNA gene sequence similarity, strain SlU-01(T) was shown to belong to the family Caulobacteraceae and was most closely related to Phenylobacterium immobile (96(.)6 %) and Phenylobacterium lituiforme (96(.)5 %). The genomic DNA G + C content of strain SlU-01(T) was 68(.)1 mol%, within the range of 67(.)3-68(.)4 mol% for the genus Phenylobacterium. Chemotaxonomic data (major ubiquinone, Q-10; major fatty acids C(18:1)omega 7c, C-16:0, C-15:0 and C-17:0) and 16S rRNA gene sequence analysis supported the affiliation of strain SlU-01(T) to the genus Phenylobacterium. However, DNA-DNA hybridization data and phenotypic properties showed that strain Slu-01(T) could be distinguished from the two other Phenylobacterium species with validly published names. Thus, it is suggested that strain Slu-01(T) represents a novel species of the genus Phenylobacterium, for which the name Phenylobacterium koreense is proposed. The type strain is SlU-01(T) (=KCTC 12206(T) = IAM 15119(T))
Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates
No full textA plate assay based on the visible solubilization of small Substrate particles and the formation of haloes oil Petri dishes, containing a mixture of different dye-labelled polysaccharides Lis substrates, provides a specific, reliable and rapid simultaneous detection of corresponding polysaccharide-degrading microorganisms. It has potential for increasing the efficacy of screening of microorganisms, utilizing different polysaccharides, in large numbers of natural samples. Diversely colored insoluble forms of amylose, xylan and hydroxyethyl-cellulose (HE-cellulose) were prepared as chromogenic substrates by using the cross-linking reagent 1,4-butanediol diglycidyl ether and the dyes Brilliant Red 3B-A, Cibacron Blue 3GA and Reactive Orange 14. Using the method, the bacteria with amylase or xylanase or cellulase or a combination of these activities were screened from soil and sludge samples, selected and identified according to 16S rDNA sequencing. (C) 2003 Elsevicr B.V. All Lights reserved
Pleomorphomonas koreensis sp nov., a nitrogen-fixing species in the order Rhizobiales
No full textA Gram-negative, non-motile, non-spore-forming, rod-shaped bacterium (strain Y9(T)) was isolated from a contaminated culture of the phototrophic bacterium Rhodopseudomonas palustris, and was investigated using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y9(T) belonged to the order Rhizobiales in the Alphaproteobacteria. Comparison of phylogenetic data indicated that it was most closely related to Pleomorphomonas oryzae (98(.)5% similarity of 16S rRNA gene sequence), and the phylogenetic distance from any other species of the order Rhizobiales with a validly published name was greater than 7(.)5% (i.e. less than 92(.)5% similarity). The predominant ubiquinone was Q-10 and the major fatty acids were C-18:1, C-16:0, C-19:0 cyclo omega 8c and C-18:0. The G + C content of genomic DNA of strain Y9(T) was 65(.)1 mol%. The results of DNA-DNA hybridization in combination with chemotaxonomic and physiological data demonstrated that strain Y9(T) represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas koreensis sp. nov. is proposed. The type strain is Y9(T) (=KCTC 12246(T) =NBRC 100803(T)
Methylobacterium jeotgali sp nov., a nonpigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood
No full textA novel facultatively methylotrophic, Gram-negative, rod-shaped bacterium, designated strain S2R03-9(T), was isolated from jeotgal, a traditional Korean fermented seafood. The organism was strictly aerobic, motile by means of a single polar flagellum, non-sporulating and catalase- and oxidase-positive. Strain S2RO3-9(T) grew in the presence of 0-1 % (w/v) NaCl and at pH 6.0-10.0, with optimum growth in the absence of NaCl and at pH 7.0. It grew at temperatures in the range 20.0-30.0 degrees C, with optimum growth at 30 degrees C. Colonies grown on R2A medium were nonpigmented, opaque and creamy white. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it was most closely related to Methylobacterium organophilum JCM 2833(T) (96.6% similarity) and the phylogenetic similarities to all other Methylobacterium species with validly published names were less than 95.0%. The DNA G + C content was 64.9 mol%. The phylogenetic analysis, the phenotypic assessment and the chemotaxonomic data (major ubiquinone, Q-10; major fatty acids, C-18:1 and C-18:0) showed that S2RO3-9(T) represents a novel species within the genus Methylobacterium in the class Alphaproteobacteria, for which the name Methylobacterium jeotgali sp. nov. is proposed. The type strain is S2RO3-9(T) (= KCTC 12671(T) = LMG 23639(T))
Starkeya koreensis sp nov., isolated from rice straw
No full textA facultatively chemolithoautotrophic, Gram-negative, aerobic, highly curved rod-shaped, nonmotile, non-spore-forming bacterium, strain Jip08(T), was isolated from rice straw in Daejeon, South Korea. Strain Jip08(T) produced colourless colonies (1.0-1.5 mm) on R2A medium after 3 days. On the basis of 16S rRNA gene sequence similarity, strain Jip08(T) was shown to belong to the alpha-2 subclass of the Proteobacteria, and was most closely related to Starkeya novella IAM 12100(T) (98.6%), Angulomicrobium tetraedale DSM 5895(T) (98.1%), Angulomicrobium amanitiforme NCIMB 1785(T) (98.0%), Ancylobacter aquaticus ATCC 25396(T) (96.6%) and Methylorhabdus multivorans DM13(T) (95.8%), all of which belong to the family Hyphomicrobiaceae. The genomic DNA G + C content of strain Jip08(T) was 69 mol%. Chemotaxonomic data [major ubiquinone, Q-10; major fatty acids, C-18:1 isomer (summed feature of C-18:1 omega 7c/omega 9t/omega 12t), C-19:0 cyclo omega 8c and C-16:0], 16S rRNA gene sequence analyses and some morphological and physiological features supported the affiliation of strain Jip08(T) to the genus Starkeya. However, DNA-DNA hybridization data and some phenotypic properties showed that strain Jip08(T) could be distinguished from the single known Starkeya species and represented a novel species, for which the name Starkeya koreensis sp. nov. is proposed. The type strain is Jip08(T) (= KCTC 12212(T) = NBRC 100963(T) = IAM 15215(T))
Novel insoluble dye-labeled substrates for screening inulin-degrading microorganisms
No full textInulin hydrogels were used to prepare insoluble colored substrates for screening of inulin-degrading microorganisms. Methycrylated inulin, synthesized with glycidyl methacrylate, was crossed-linked by free radical polymerization producing an insoluble hydrogel. The inulin hydrogels were colored with one of three covalent dyes (Reactive blue 4, Reactive orange 14, Reactive red 120). The efficacy of the colored inulin hydrogels was determined by comparing their performance to insoluble, colored amylose, xylan and HE-cellulose in screening assays. Novel substrates (alone or in combination with other insoluble colored substrates) were successfully used for screening inulin-degrading microorganisms from sludge samples. Selected strains were identified using 16S rDNA gene partial sequencing; all of them belong to the genus Bacillus. (C) 2007 Elsevier B.V. All rights reserved
Paracoccus koreensis sp nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket (UASB) reactor
No full textA Gram-negative, short rod- to coccus-shaped, non-spore-forming bacterium (Ch05(T)) was isolated from granules in an upflow anaerobic sludge blanket (UASB) reactor. On the basis of 16S rRNA gene sequence similarity, strain Ch05(T) was shown to belong to the subclass alpha-Proteobacteria, being related to Paracoccus solventivorans (97.5%), Paracoccus alkenifer (96.9%) and Paracoccus kocurii (96.4%). The phylogenetic distance from Paracoccus species with validly published names was always less than 96%. Physiological and chemotaxonomic data (major ubiquinone, Q-10; major fatty acids, C-18:1 and C-18:0) supported the affiliation of strain Ch05(T) to the genus Paracoccus. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Ch05(T) from the 17 Paracoccus species with validly published names. Ch05(T) therefore represents a novel species, for which the name Paracoccus koreensis sp. nov. is proposed. The type strain is Ch05(T) (= KCTC 12238(T) = IAM 15216(T))
- …
