1,809 research outputs found
The construction of Karen Karnak: The multi-author-function
No full textThis thesis is situated within the comparatively recent developments of Web 2.0 and the emergence of interactive WikiMedia, and explores the mode of authorship within a Read/Write culture compared to that of a Read/Only tradition. The hypothesis of this study is that the role of the audience has become merged with the author, and as such, represents new functions and attributes, distinct from a more conventional concept of authorship, in which the roles of audience and author are more separate. Read/Write and participatory culture, as defined by this study, is focused on collaboration, and includes the influences of D.I.Y. culture, Open-Source practices and the production of text by multiple authors. Multi-authorship presents a re-thinking of several concepts which support the notion of the individual author, since the focus of multi-authorship is not on attribution and ownership of a finished text, but on the continued malleability of a text. Modes of multi-authorship, demonstrated in the use of the pseudonyms Alan Smithee and Karen Eliot, represent declarative authors whose names signify multiple origins, whilst concurrently indicating a distinct body of work. The function of these names form an important context to this study, since primary research involves the construction of an experimental mode of multi-authorship utilising WikiMedia technology and the interaction of thirty nine participants, who are invited to create a body of work under the collective pseudonym Karen Karnak. The data generated by this experiment is analysed using aspects of Michel Foucault's author-function to identify and determine power structures inherent in the WikiMedia context. The interplay of power structures, including concepts such as identity, ownership and the body of work, affect the resulting mode of authorship and contribute to the construction of Karen Karnak, suggesting further areas of research into the emerging multi-author
Challenging the "chromatin hypothesis" of cardiac laminopathies with LMNA mutant iPS cells
No full textLamins A and C are intermediate filaments that provide structural support to the nuclear envelope and regulate gene expression. In this issue, Bertero et al. (2019. J. Cell Biol. https://doi.org/10.1083/jcb.201902117) report that although lamin A/C haploinsufficient cardiomyocytes show disease-associated phenotypes, those changes cannot be explained by alterations in chromatin compartmentalization
FSD-FS
No full textFSD-FS is a publicly-available database of human labelled sound events for few-shot learning. It spans across 143 classes obtained from the AudioSet Ontology and contains 43805 raw audio files collected from the FSD50K. FSD-FS is curated at the Centre for Digital Music, Queen Mary University of London.
Citation
If you use the FSD-FS dataset, please cite our paper and FSD50K.
@article{liang2022learning,
title={Learning from Taxonomy: Multi-label Few-Shot Classification for Everyday Sound Recognition},
author={Liang, Jinhua and Phan, Huy and Benetos, Emmanouil},
journal={arXiv preprint arXiv:2212.08952},
year={2022}
}
@ARTICLE{9645159, author={Fonseca, Eduardo and Favory, Xavier and Pons, Jordi and Font, Frederic and Serra, Xavier}, journal={IEEE/ACM Transactions on Audio, Speech, and Language Processing}, title={FSD50K: An Open Dataset of Human-Labeled Sound Events}, year={2022}, volume={30}, number={}, pages={829-852}, doi={10.1109/TASLP.2021.3133208}}
About FSD-FS
FSD-FS is an open database for multi-label few-shot audio classification containing 143 classes drawn from the FSD50K. It also inherits the AudioSet Ontology. FSD-FS follows the ratio 7:2:1 to split classes into base, validation, and evaluation sets, so there are 98 classes in the base set, 30 classes in the validation set, and 15 classes in the evaluation set (More details can be found in our paper).
LICENSE
FSD-FS are released in Creative Commons (CC) licenses. Same as FSD50K, each clip has its own license as defined by the clip uploader in Freesound, some of them requiring attribution to their original authors and some forbidding further commercial reuse. For more details, ones can refer to the link.
FILES
FSD-FS are organised in the structure:
root
|
└─── dev_base
|
└─── dev_val
|
└─── eval
REFERENCES AND LINKS
[1] Gemmeke, Jort F., et al. "Audio set: An ontology and human-labeled dataset for audio events." 2017 IEEE international conference on acoustics, speech and signal processing (ICASSP). IEEE, 2017. [paper] [link]
[2] Fonseca, Eduardo, et al. "Fsd50k: an open dataset of human-labeled sound events." IEEE/ACM Transactions on Audio, Speech, and Language Processing 30 (2021): 829-852. [paper] [code
Lineage Plasticity and Regenerative Potential of Adult Muscle Stem Cells: Investigation of Satellite Cell Direct-Reprogramming and Pericyte Self-Renewal
No full textSatellite cells are responsible for most of adult skeletal muscle regeneration. Upon activation they differentiate into transient amplifying myoblasts that undergo cell fusion to form multinucleated fibres. Despite their remarkable differentiation ability and the positive outcomes obtained with transplantation in dystrophic mice and recently in patients with oculo-pharyngeal muscular dystrophy (OPMD), clinical trials in patients with Duchenne muscular dystrophy (DMD) showed limited efficacy, mainly ascribed to myoblasts low survival and poor migration ability. Muscle pericyte-derived mesoangioblasts (perivascular cells associated to the capillaries) also contribute to muscle regeneration and colonise the satellite cell niche. These cells can be injected systemically and migrate through the vascular endothelium, circumventing the necessity of multiple intra-muscular injections. Mesoangioblasts have been also tested in a recently completed phase I / II clinical trial to assess their safety profile in five DMD patients (EudraCT no. 2011-000176-33). We hypothesise that exploiting the key properties of myoblasts and mesoangioblasts may have the potential to produce clinically relevant cells, superior to those currently available. This work shows that exposure to molecules involved in pericyte specification such as the Notch ligand DLL4 and the growth factor PDGF-BB can induce direct reprogramming of primary satellite cells to pericyte-like cells. Reprogrammed cells acquire perivascular marker expression without losing the satellite cell marker Pax7. These highly myogenic cells can be expanded in culture and showed increased engraftment. In vitro and in vivo experiments also showed improved migration ability, similar to what has been observed with mesoangioblasts. Additionally, this thesis includes a set of experiments aiming to assess the self-renewal potential of mesoangioblast-derived cells via serial transplantation assays. Overall, the results obtained improve our understanding of smooth / skeletal fate choice and self-renewal, providing evidence of the possibility of exploiting a direct reprogramming approach to allow systemic delivery of myoblasts for cell therapies of muscular dystrophies
Tissue architecture of the anterior pituitary showing the epithelial cell cords with hormonal cells and folliculo-stellate (FS) cells, the capillaries (C) with fenestrated endothelial cells (EC) and connective tissue (CT)
No full text<p><b>Copyright information:</b></p><p>Taken from "Paracrinicity: The Story of 30 Years of Cellular Pituitary Crosstalk"</p><p></p><p>Journal of Neuroendocrinology 2008;20(1):1-70.</p><p>Published online Jan 2008</p><p>PMCID:PMC2229370.</p><p>© 2008 The Author. Journal Compilation © 2008 Blackwell Publishing Ltd</p> The cell cords are a cluster of endocrine cells surrounding an aggregate of FS cells that make a follicle (F). FS cells also make a meshwork between the hormonal cells, making junctions among each other (thick lines) and extending foot processes (f) ending on the basal membrane (BM) in the periphery of the cord. The cords are surrounded by BM, which may have extensions between some cells. A second BM surrounds the capillary vessels and between these two some connective tissue resides. Small and larger lacunae are present between hormonal cells. Paracrine substances may circulate from cell-to-cell but also could be released in these lacunae and reach more remote places. FS cells make gap junctions mostly among each other, but occasionally also with some hormonal cells. Hormonal cells can make interdigitations with FS cells (small arrows) to favour cell-to-cell communication. Adapted from Vila-Porcile ()
Adaptive pulse compression for transform-limited 15-fs high-energy pulse generation
No full textWe demonstrate the use of a deformable-mirror pulse shaper, combined with an evolutionary optimization algorithm, to correct high-order residual phase aberrations in a 1-mJ, 1-kHz, 15-fs laser amplifier. Frequency resolved optical gating measurements reveal that the output pulse duration of 15.2 fs is within our measurement error of the theoretical transform limit. This technique significantly reduces the pulse duration and the temporal prepulse energy of the pulse while increasing the peak intensity by 26%. It is demonstrated, for what is believed to be the first time, that the problem of pedestals in laser amplifiers can be addressed by spectral-domain correction
Generation of Biocompatible Human 3D Skeletal Muscle Tissue from Healthy and Dystrophic Pluripotent Stem Cells
No full textSkeletal muscle is a tissue with remarkable regenerative abilities but a wide range of disorders can impair its functions. At present, there is no effective therapy for most of these pathologies and this represents a significant unmet medical need. The pursuit of therapeutic strategies has led to the development of different encouraging approaches. Importantly, tissue engineering is also emerging as a promising discipline for the establishment of new platforms with therapeutic relevance for muscle disorders. Generating human artificial skeletal muscles in vitro would indeed provide an invaluable tool for disease modelling, drug screening and tissue replacement. Nevertheless, skeletal muscle tissue engineering is extremely challenging and at present no commonly endorsed model is available. Specifically, one of the bottlenecks is the use of primary cells, which hold the drawback of scarce availability and reduced proliferation and differentiation potential in vitro thus limiting their use. Here I describe the generation of 3D artificial mini-muscles from human pluripotent stem cells derived from healthy donors and patients with muscular dystrophy. This has never been reported before and the use of pluripotent stem cells offers a virtually unlimited source of myogenic cells. These patient- and disease-specific human artificial mini-muscles recapitulate characteristics of the adult tissue and, importantly, are able to engraft into immunodeficient mice. Finally, I show that other isogenic cell types present in normal muscle tissue (such as endothelial cells and pericytes) can be derived and combined together with the same patient-specific myogenic cells, thus generating a multi-lineage artificial mini-muscle. This complex platform could provide a valuable tool for skeletal muscle disease modelling, drug screening and tissue replacement ultimately leading to the development of new therapies for muscle diseases
Human artificial chromosomes for Duchenne muscular dystrophy and beyond: challenges and hopes.
No full textSafe and efficacious vectors able to carry large or several transgenes are of key importance for gene therapy. Human artificial chromosomes can fulfil this essential requirement; moreover, they do not integrate into the host genome. However, drawbacks such as the low efficiency of chromosome transfer and their relatively complex engineering still limit their widespread use. In this article, I summarise the key steps that brought human artificial chromosomes into preclinical research for Duchenne muscular dystrophy, an X-linked, monogenic disorder. I will also review possible future pre-clinical and clinical perspectives for this technology
Self-compression of 4.9 µm pulses to sub-40 fs with 2 mJ energy in Zinc Sulfide
No full textNonlinear self-compression of few-cycle multi-mJ pulses at 4.9 µm in ZnS is presented. 80 fs input pulses are compressed to 37 fs with 2.1 mJ energy at a 1 kHz repetition rate. © 2024 The Author(s
Sub-40 fs Er:fiber laser
No full textSimulation and experiment results are presented for sub-40 fs pulse generation in Er: fiber ring lasers. (C) 2010 Optical Society of Americahttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000295612401079&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Engineering, Electrical & ElectronicOpticsPhysics, AppliedCPCI-S(ISTP)
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