1,902 research outputs found
Supplemental_Material – Supplemental material for Examining the relationships between environmental barriers and leisure in community-dwelling individuals living with stroke
No full textSupplemental material, Supplemental_Material for Examining the relationships between environmental barriers and leisure in community-dwelling individuals living with stroke by Ada Tang, Billy Sun, Marco YC Pang and Jocelyn E Harris in Clinical Rehabilitation</p
Subtyping of human T-lymphotropic virus type I by amplification of long terminal repeat sequences and restriction fragment length polymorphism analysis in carriers with multiple transfusion
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Yc-1 Attenuates Lps-Induced Proinflammatory Responses and Activation of Nuclear Factor-Kappa B in Microglia
No full textBackground and purpose: An inflammatory response in the central nervous system mediated by the activation of microglia is a key event in the early stages of the development of neurodegenerative diseases. LPS has been reported to cause marked microglia activation. It is very important to develop drugs that can inhibit microglia activation and neuroinflammation. Here, we investigated the inhibitory effect of YC-1, a known activator of soluble guanylyl cyclase, against LPS-induced inflammatory responses in microglia. Experimental approach: To understand the inhibitory effects of YC-1 on LPS- induced neuroinflammation , primary cultures of rat microglia and the microglia cell line BV-2 were used. To examine the mechanism of action of YC-1, LPS- induced nitric oxide ( NO) and prostaglandin E-2( PGE (2)) production, iNOS, COX-2 and cytokine expression were analyzed by Griess reaction, ELISA, Western blotting and RT-PCR, respectively. The effect of YC-1 on LPS- induced activation of nuclear factor kappa B (NF- kappa B) was studied by NF-kappa B reporter assay and immunofluorocytochemistry. Key results: YC-1 inhibited LPS- induced production of NO and PGE2 in a concentration- dependent manner. The protein and mRNA expression of iNOS and COX-2 in response to LPS application were also decreased by YC-1. In addition, YC-1 effectively reduced LPS- induced expression of the mRNA for the proinflammatory cytokines, TNF-alpha and IL-1 beta. Furthermore, YC-1 inhibited LPS- induced NF-kappa B activation in microglia. Conclusions and implications: YC-1 was able to inhibit LPS- induced iNOS and COX-2 expression and NF- kappa B activation, indicating that YC-1 may be developed as an anti- inflammatory neuroprotective agent. British Journal of Pharmacology ( 2007 )
Effects of YC-1 on Hypoxia-Inducible Factor 1 Alpha in Hypoxic Human Bladder Transitional Carcinoma Cell Line T24 Cells
No full text<i>Objectives:</i> It was the aim of this study to explore the effects of 3-(5′-hydroxymethyl-2′-furyl)-l-benzyl indazole (YC-1) on transcription activity, cell proliferation and apoptosis of hypoxic human bladder transitional carcinoma cells (BTCC), mediated by hypoxia-inducible factor 1α (HIF-1α). <i>Methods:</i> BTCC cell line T24 cells were incubated under normoxic or hypoxic conditions, adding different doses of YC-1. The protein expression of HIF-1α and HIF-1α-mediated genes was detected by Western blotting. RT-PCR was used to detect HIF-1α mRNA expression. Cell proliferation, apoptosis and migration activity were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, flow cytometry and transwell migration assay. The cells were pretreated by two ERK/p38 MAPK pathway-specific inhibitors, PD98059 or SB203580, and then incubated with YC-1 treatment under hypoxic condition. HIF-1α protein expression was detected by Western blotting. <i>Results:</i> Hypoxic T24 cells expressed a higher level of HIF-1α, vascular endothelial growth factor, matrix metalloproteinases-2, B-cell lymphoma/leukemia-2 protein and HIF-1α mRNA compared with normoxic controls, in which the above-mentioned expression was downregulated by YC-1 in a dose-dependent manner. Cell proliferation and migration activity were inhibited while apoptosis was induced by YC-1 under hypoxic condition. Moreover, YC-1-downregulated HIF-1α expression was reversed by PD98059 and SB203580, respectively. <i>Conclusions:</i> YC-1 inhibits HIF-1α and HIF-1α-mediated gene expression, cell proliferation and migration activity and induces apoptosis in hypoxic BTCC. The ERK/p38 MAPK pathway may be involved in YC-1-mediated inhibition of HIF-1α.</jats:p
Use of Herbal Dietary Supplement Si-Wu-Tang and Health-Related Quality of Life in Postpartum Women: A Population-Based Correlational Study
No full text[[abstract]]Objective. The aim of the study was to explore the association between women’s use of herbal dietary supplement Si-Wu-Tang during the postpartum period and their health-related quality of life. Methods. This is a population-based correlational study. We used multistage, stratified, systematic sampling to recruit 24,200 pairs of postpartum women and newborns from the Taiwan National Birth Registry in 2005. A structured questionnaire was successfully administered to 87.8% of the sampled population. Trained interviewers performed home interviews 6 months after the women’s deliveries between June 2005 and July 2006. The Medical Outcomes Study 36-item Short-Form (SF-36) was used to measure the quality of life of the women along with the frequency of Si-Wu-Tang use. Results. Si-Wu-Tang use after delivery improved women’s score for bodily pain and also improved their score for mental health when used more than 10 times. In addition, there were increases in general health and vitality scores in the group who continuously used Si-Wu-Tang more than 10 times after using Sheng-Hua-Tang. Conclusion. Use of Si-Wu-Tang after delivery may be associated with women’s health-related quality of life especially for those who previously used Sheng-Hua-Tang. These results are exploratory and need to be replicated
Revascularization of turnover sternum: A definitive treatment for intractable funnel chest.
No full textPartitioning of Poly(amidoamine) Dendrimers between n-Octanol and Water
No full textDendritic nanomaterials are emerging as key building blocks for a variety of nanoscale materials and technologies. Poly(amidoamine) (PAMAM) dendrimers were the first class of dendritic nanomaterials to be commercialized. Despite numerous investigations, the environmental fate, transport and toxicity of PAMAM dendrimers is still not well understood. As a first step toward the characterization of the environmental behavior of dendrimers in aquatic systems, we measured the octanol-water partition coefficients (logK(ow)) of a homologous series of PAMAM dendrimers as a function of dendrimer generation (size),terminal group and core chemistry. We find that the log K(ow) of PAMAM dendrimers depend primarily on their size and terminal group chemistry. For G1-G5 PAMAM dendrimers with terminal NH(2) groups, the negative values of their log K(ow) indicate that they prefer to remain in the water phase. Conversely, the formation of stable emulsions at the octanol-water (O/W) interface in the presence of G6-NH(2) and G8-NH(2) PAMAM dendrimers suggest they prefer to partition at the O/W interface. In all cases, published studies of the cytotoxicity of Gx-NH(2) PAMAM dendrimers show they strongly interact with the lipid bilayers of cells. These results suggest that the log K(ow) of a PAMAM dendrimer may not be a good predictor of its affinity with natural organic media such as the lipid bilayers of cell membranes
Safety and immunogenicity of a Diphtheria, Tetanus, and acellular pertussis-inactivated poliovirus vaccine/Haemophilus Influenzae type B combination Vaccine administered to Taiwanese infants at 2, 4 and 6 months of age
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