13,840 research outputs found
Cell-specific regulation of gene expression and transactivation of E2F site-containing promoters by SV40 small t antigen
A new species of Aleurolobus Quaintance et Baker (Homoptera, Aleyrodidae) from Southern Europe.
Aleurolobus teucrii n. sp. is described from southern Italy and the Maltese Islands (Central Mediterranean). The species seems to be monophagous on Teucrium fruticans L. A key to the European species of this genus (A. niloticus Priesner et Hosny, A. olivinus (Silvestri), A. wunni (Ryberg) and A. teucrii n. sp.) is provided.peer-reviewe
Analysis of local tAI profiles.
<p>(<b>A</b>) A schematic description of the 5 segments, each for 30 codons from the N′-terminal region and the C′-terminal of the coding sequence. (<b>B</b>) Relative tAI profile of the N′- and C′-terminal segments of the human proteome according to 4 group partition (as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003294#pcbi.1003294.s004" target="_blank">Table S2</a>). Each of the protein group is color coded as follows: Red, SP non-TMD; Black, SP and TMD; Green, TMD non-SP; Blue, Cytosolic proteins. Purple, the entire proteome, marked as “All.” Pink asterisks, the random proteins according to length distribution of the proteome. (<b>C</b>) Cumulative distribution of proteins according to the tAI values of the N1 and C1 segments. The data are based on all tAI values that were compiled in (B) for N′- and C′-termini. Note that for the N′-terminal but not the C′-terminal, the cumulative distribution of each of the four protein groups is distinctive. The statistic of the cumulative distribution for human proteome is shown in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1003294#pcbi-1003294-t002" target="_blank">Table 2</a>. Relative tAI profile for <i>B. taurus</i> (<b>D</b>), <i>D. melanogaster</i> (<b>E</b>), <i>C. elegans</i> (<b>F</b>) and <i>S. cerevisiae</i> (<b>G</b>). (<b>H</b>) The range of relative tAI values of N1, N3, and C1 segments of all tested organisms. The relative tAI range is defined as the highest averaged relative tAI subtracted by the lowest averaged relative tAI value among the four protein groups within the same segment.</p
Biomolecula imaging based on far-red fluorescent protein with a high two-photon excitation action cross section.
Asticcacaulis solisilvae sp nov., isolated from forest soil
An obligately aerobic, chemoheterotrophic, mesophilic prosthecate bacterium, designated strain CGM1-3EN(T), was isolated from the enrichment cultures of forest soil from Cheonggyesan Mountain, Republic of Korea. Cells were Gram-reaction-negative, motile rods (1.3-2.4 mu m long by 0.30-0.75 mu m wide) with single flagella. The strain grew at 10-37 degrees C (optimum 25-30 degrees C) and at pH 4.5-9.5 (optimum 5.0-7.0). The major cellular fatty acids were C-16:0, C-18:1 omega 7c 11-methyl, C-12:1 3-OH and summed feature 8 (comprising C-18:1 omega 7c/C-18:1 omega 6c). The genomic DNA G + C content of strain CGM1-3EN(T) was 63.7 mol%. The closest phylogenetic neighbour to strain CGM1-3EN(T) was identified as Asticcacaulis biprosthecium DSM 4723(T) (97.2 % 16S rRNA gene sequence similarity) and the DNA-DNA hybridization value between strain CGM1-3EN(T) and A. biprosthecium DSM 4723(T) was less than 24.5 %. Strain CGM1-3EN(T) used D-glucose, D-fructose, sucrose, maltose, trehalose, D-mannose, D-mannitol, D-sorbitol, D-galactose, cellobiose, lactose, raffinose, fumarate, pyruvate, DL-alanine and glycerol as carbon sources. Based on data from the present polyphasic study, the forest soil isolate CGM1-3EN(T) is considered to represent a novel species of the genus Asticcacaulis, for which the name Asticcacaulis solisilvae sp. nov. is proposed. The type strain is CGM1-3EN(T) (=AIM0088(T)=KCTC 32102(T)=JCM 18544(T)).OAIID:oai:osos.snu.ac.kr:snu2013-01/102/0000002410/17SEQ:17PERF_CD:SNU2013-01EVAL_ITEM_CD:102USER_ID:0000002410ADJUST_YN:YEMP_ID:A002014DEPT_CD:458CITE_RATE:2.112DEPT_NM:화학생물공학부SCOPUS_YN:YCONFIRM:YN
Mosquito Larvicidal Constituents from Lantana Viburnoides SP Viburnoides Var Kisi (A. rich) Verdc (Verbenaceae).
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Lantana viburnoides sp viburnoides var kisi is used in Tanzania ethnobotanically to repel mosquitoes as well as in traditional medicine for stomach ache relief. Bioassay-guided fractionation and subtraction bioassays of the dichloromethane extract of the root barks were carried out in order to identify the bioactive components for controlling Anopheles gambiae s.s. mosquito larvae. Twenty late III or early IV instar larvae of An. gambiae s.s. were exposed to various concentrations of the plant extracts, fractions, blends and pure compounds, and were assayed in the laboratory by using the protocol of WHO 1996. Mean mortalities were compared using Dunnett's test (p < 0.05) and lethal concentration calculated by Lackfit Inversel of the SAS programme. The crude extract (LC50 = 7.70 ppm in 72 h) and fractions exhibited different level of mosquito larvicidal activity with subtraction of some fractions resulting in activity enhancement. The active fractions contained furanonaphthaquinones regio-isomers (LC50 = 5.48-5.70 ppm in 72 h) and the lantadene triterpenoid camaric acid (LC50 = 6.19 ppm in 72 h) as active principles while the lupane triterpenoid betulinic acid (LC50 < 10 ppm in 72 h) was obtained from the least active fraction. Crude extracts and some fractions had higher or comparable larvicidal activity to the pure compounds. These results demonstrate that L. viburnoides sp viburnoides var kisi extracts may serve as larvicides for managing various mosquito habitats even in their semi-purified form. The isolated compounds can be used as distinct markers in the active extracts or plant materials belonging to the genus Lantana
Optical signal degradation study in fixed human skin using confocal microscopy and higher-harmonic optical microscopy
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