1,721,071 research outputs found
Recent advances in the application of CE to forensic sciences, an update over years 2009-2011
No full textThe present article reviews and comments the applications of capillary electrophoresis in the different areas of forensic sciences covering the time from the second half of 2009 until the first part of 2011, being the latest update of previous reviews covering the years from 2001 to 2009. Numerous articles reporting applications of capillary electrophoresis to analytical problems of potential interest for the forensic researchers and scientists can be found in the most qualified journals of analytical chemistry, analytical biochemistry, pharmacology, toxicology, laboratory medicine, human genetics, etc. However, the present review has been focused on discussing only the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques published in the following fields: (i) illicit and abused drugs, (ii) ions and small molecules of forensic interest, (iii) proteins and peptides of forensic interest, (iv) dyes and inks, (v) forensic DNA. The present review collects and comments on 60 references. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Guidelines for Testing Drugs under International Control in Hair, Sweat and Oral Fluid
No full textThe present Manual is a revision of the United Nations Office on Drugs and Crime (UNODC) manual Guidelines for Testing Drugs Under International Control (ST/NAR/30/Rev.2). This version has been prepared taking into account recent developments in analytical technology to detect conventional and new, unconventional drugs and is based on up-to-date scientific knowledge of the physiology and pharmacology
of the so-called “alternate” biological specimens, which today may offer important information, complementary to the analysis of the traditional biological specimens (blood and urine)
Il delitto di doping in Medicina legale per i corsi di laurea a orientamento giuridico e triennali
No full textNano-liquid chromatography for enantiomers separation of baclofen by using vancomycin silica stationary phase.
No full textThe chiral separation of baclofen (Bac) was obtained by nano-liquid chromatography tandem mass spectrometry (nano-LC-MS/MS) using a 100 μm I.D. fused silica capillary column packed with silica particles chemically modified with vancomycin. Various experimental parameters, such as composition (buffer concentration, water content, organic modifier) and pH of the mobile phase and sample solvent were investigated for method optimization. In order to increase the sensitivity an on-column focusing procedure was applied. Acceptable separation of Bac enantiomers was obtained in less than 11 min eluting in isocratic mode, with 90:10 MeOH/water (v/v) containing 10 mM ammonium acetate at pH 4.5. These optimized experimental conditions were applied to the analysis of human plasma samples spiked with racemic mixture of Bac. The use of a Buckypaper disc as sorbent membrane allows one to recover both enantiomers with yields ≥ 65%. The method was fully validated, following the identification criteria of the European Commission Decision 2002/657/EC
Capitolo 23 'Il delitto di doping'. In Medicina legale. Per studenti e medici di medicina generale
No full textLactate determination in human vitreous humour by capillary electrophoresis and time of death investigation
No full textForensic inquests, particularly, in assessing time since death currently recognize the importance of the analysis of vitreous humour (VH) biomarkers. Present research, studies, and validates the determination of lactate (La) in VH by CZE with indirect UV detection. The BGE (pH 8.9) consisted of Tris buffer (37 mM) containing 4-methoxybenzoic acid (4 mM) and alkyl-trimethyl-ammonium bromide (1.2 mM). Each VH specimen was diluted with a butyric acid solution (internal standard 0.057 mM) and La and butyrate were separated within 3-5 min (30 kV). The La LOQ and LOD were 4 and 2 mM, respectively. The calibration curve linearity ranged from 4 to 80 mM; intra- and interruns precisions were less than 10% for standard as well as for VH specimen, respectively. To investigate postmortem interval (PMI) and VH lactate level correlation, human VH specimens were collected during autopsy (n = 40) and stored at -20°C until assay. La levels ranged from 16 to 42 mM; PMI values ranged from 10 to 141 h. La (mM) and PMI (h) correlation was statistically significant (r2 = 0.527; p < 0.05). In conclusion, the present CZE analysis is efficacious to determine VH La as a biomarker for PMI investigatio
First application of atmospheric-pressure chemical ionization gas chromatography tandem mass spectrometry to the determination of cannabinoids in serum.
No full textThe analysis of cannabinoids in blood samples is still a challenging issue for forensic laboratories, because of the low concentrations to be determined to prove that a person acted under CannabisTherefore, sensitive analytical techniques are required. This study presents the development and validation of a novel APGC-MS/MS method for the simultaneous determination of Δ9-tetrahydrocannabinol (THC), 11-hydroxy- Δ9-THC (THC-OH), 11-nor-9-carboxy- Δ9-THC (THCA), cannabidiol (CBD), cannabidiol acid (CBDA) and cannabigerol (CBG) in human serum. The developed method was fully validated according to international guidelines, with evaluation of selectivity, precision, accuracy, linearity, LODs and LOQs, extraction recovery and matrix effect. The method was linear in the range 0.2-25 ng/mL for THC, THC-OH, CBD and CBG, while for THCA and CBDA linearity was assessed in the range of 0.8-100 ng/mL and 3-100 ng/mL, respectively. The LOQs were determined in 0.2 ng/mL for THC, 0.4 ng/mL for THC-OH, 0.8 ng/mL for CBD and CBG, 1.6 ng/mL for THCA and 3 ng/mL for CBDA. The method was applied to the analysis of 15 serum samples from DUID cases. To the best of our knowledge, the present work is the first one describing an application of APGC source in the field of forensic toxicology
Rapid optimized separation of bromide in serum samples with capillary zone electrophoresis by using glycerol as additive to the background electrolyte
No full textAfter decades of neglect, bromide has recently been re-introduced in therapy as an effective anti-epileptic drug. The present paper describes the methodological optimization and validation of a method based on capillary zone electrophoresis for the rapid determination of bromide in serum using a high-viscosity buffer and a short capillary (10 cm). The optimized running buffer was composed of 90 mM sodium tetraborate, 10 mM sodium chloride, pH 9.24 and 25% glycerol. The separation was carried out at 25 kV at a temperature of 20 °C. Detection was by direct UV absorption at 200 nm wavelength. The limit of detection (signal-to-noise ratio = 5) in serum was 0.017 mM. The precision of the method was verified in blank serum samples spiked with bromide, obtaining intra-day and day-to-day tests, relative standard deviation values ≤0.2% in terms of migration times and values <2% in terms of peaks areas, respectively
Analytical and diagnostic aspects of carbohydrate deficient transferrin (CDT): A critical review over years 2007-2017.
No full textThe need for investigating alcohol abuse by means of objective tools is worldwide accepted. Among the currently available biomarkers of chronic alcohol abuse, carbohydrate-deficient transferrin (CDT) is one of the most used indicator, mainly because of its high specificity. However, some CDT analytical and interpretation aspects are still under discussion, as witnessed by numerous research papers and reviews. The present article presents a critical review of the literature on CDT appeared in the period from 2007 to 2017 (included). The article is organized in the following sections: (1) introduction, (2) pre-analytical aspects (3) analytical aspects (4) diagnostic aspects (5) concluding remarks. As many as 139 papers appeared in the international literature and retrieved by the search engines PubMed, Web of Science and Scopus are quoted
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