22,886 research outputs found
Sphingopyxis flavimaris sp. nov., isolated from sea water of the Yellow Sea in Korea
No full textA Gram-negative, motile, yellow-pigmented, slightly halophilic bacterial strain, SW-151T, was isolated from sea water of the Yellow Sea in Korea, and subjected to a polyphasic taxonomic study. The isolate grew optimally at 30 °C and in the presence of 2-3 % NaCl. Strain SW-151T was characterized chemotaxonomically as having Q-10 as the predominant respiratory lipoquinone and C18:1ω7c, C16:1ω7c and/or iso-C15:0 2-OH and C17:1ω6c as the major fatty acids. Sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the major polar lipids. The DNA G+C content was 58 mol%. 16S rRNA gene sequence analysis showed that strain SW-151T joins the evolutionary radiation enclosed by the genus Sphingopyxis. Similarities between the 16S rRNA gene sequences of strain SW-151T and the type strains of Sphingopyxis species ranged from 92.3 to 94.3%, which is low enough to categorize strain SW-151T as a species distinct from previously described Sphingopyxis species. On the basis of phenotypic properties and phylogenetic distinctiveness, strain SW-151T (=KCTC 12232T=DSM 16223T) should be classified as a novel Sphingopyxis species, for which the name Sphingopyxis flavimaris sp. nov. is proposed.open
Chryseobacterium daeguense sp. nov., isolated from wastewater of a textile dye works
No full textA Gram-negative, rod-shaped bacterial strain, K105T, was isolated from wastewater of a textile dye works in Korea and subjected to a polyphasic; taxonomic study. Strain K105T grew optimally at pH 6.5-7.5 and 30-37 °C. It contained MK-6 as the predominant menaquinone and iS0-C15:01 iSO-C17:1ω9c, iS0-C17:0 3-OH and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH) as the maior fatty acids. The DNA G+C content was 36.8 mol%. Phylogenetic: analysis based on 16S rRNA gene sequences showed that strain K105T belonged to the genus Chryseobacterium. Strain K105T exhibited 16S rRNA gene sequence similarity values of less than 96.9% to the type strains of recognized Chryseobacterium species. Strain K105T differed from phylogenetically related Chryseobacterium species in several phenotypic properties. On the basis of phenotypic and phylogenetic data, strain K105T (=KCTC 12841T=JCM 14362T) is placed in the genus Chryseobacterium as the type strain of a novel species, for which the name Chryseobacterium daeguense sp. nov. is proposed.open
Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea
No full textA novel Tenacibaculum-like bacterial strain, SIVIK-4T, was isolated from a tidal flat sediment in Korea. Strain SMK-4T was Gram-negative, pale yellow-pigmented and rod-shaped. It grew optimally at 30-37°C and in the presence of 2-3 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C15:0, iso-C16:0 3-OH and C16:1ω7c and/or iso-C15:0 2-OH as the major fatty acids (> 10 % of total fatty acids). The DNA G + C content was 33.6 mol%. Phylogenetic trees based on 16S rRNA gene sequences showed that strain SMK-4T fell within the evolutionary radiation encompassed by the genus Tenacibaculum. Strain SMK-4T exhibited 16S rRNA gene sequence similarity levels of 95.2-98.6 % with respect to the type strains of recognized Tenacibaculum species. DNA-DNA relatedness levels and differential phenotypic properties made it possible to categorize strain SMK-4T as a species that is separate from previously described Tenacibaculum species. On the basis of phenotypic properties and phylogenetic and genetic distinctiveness, strain SMK-4T (=KCTC 12569T=JCM 13491T) should be classified as a novel Tenacibaculum species, for which the name Tenacibaculum aestuarii sp. nov. is proposed.open
Pedobacter terrae sp. nov., isolated from soil
No full textA Gram-negative, non-motile, rod-shaped bacterial strain, DS-57T, was isolated from soil from Dokdo, Korea, and its taxonomic position was investigated using a polyphasic approach. It grew optimally at 25 °C and in trypticase soy broth without NaCl and trypticase soy broth with 0.5% NaCl. Strain DS-57T contained MK-7 as the predominant menaquinone and iSo-C15:0, C16:17c and/or iSo-C15:0 2-OH and iSo-C17:0 3-OH as the major fatty acids. The DNA G+C content was 39.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DS-57T was most closely related to the genus Pedobacter of the family Sphingobacteriaceae, clustering coherently with Pedobacter suwonensis, Pedobacter roseus and Pedobacter sandarakinus. Strain DS-57T exhibited 16S rRNA gene sequence similarity values of 99.2, 97.9 and 97.2 % with respect to the type strains of P. suwonensis, P. roseus and P. sandarakinus, respectively, and values less than 95.6 % with respect to the type strains of other Pedobacter species. Strain DS-57T exhibited levels of DNA-DNA relatedness of 45, 17 and 15 % with respect to the type strains of P. suwonensis, P. roseus and P. sandarakinus, respectively. Differential phenotypic properties, together with the phylogenetic distinctiveness and the DNA-DNA relatedness data, were sufficient to allow the classification of strain DS-57T as a species that is separate from recognized Pedobacter species. On the basis of phenotypic properties and phylogenetic distinctiveness, therefore, strain DS-57T represents a novel species of the genus Pedobacter, for which the name Pedobacter terrae sp. nov. is proposed. The type strain is DS-57T (=KCTC 12762T = DSM 1 7933T).open
Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand
No full textA Gram-staining-negative, motile, agarolytic bacterium, designated M-M1T, was isolated from marine sand obtained from Geoje Island, South Sea, Korea, and its taxonomic position was investigated using a polyphasic taxonomic approach. Strain M-M1T grew optimally at pH 7.0–8.0, at 30 °C and in the presence of 2 % (w/v) NaCl. It did not grow in the presence of >7 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain M-M1T fell within the clade comprising members of the genus Thalassomonas, clustering with Thalassomonas agarivorans TMA1T, Thalassomonas loyana CBMAI 722T and Thalassomonas ganghwensis JC2041T, with which it exhibited 16S rRNA gene sequence similarity values of 96.4, 96.0 and 94.9 % respectively. Strain M-M1T exhibited 94.7–95.2 % 16S rRNA gene sequence similarity to the other species of the genus Thalassomonas. Strain M-M1T contained Q-8 as the predominant ubiquinone and C16 : 1ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1ω7c as the major fatty acids. The DNA G+C content was 44.2 mol%. Strain M-M1T could be differentiated from phylogenetically related species of the genus Thalassomonas by differences in some phenotypic properties. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain M-M1T is considered to represent a novel species of the genus Thalassomonas, for which the name Thalassomonas agariperforans sp. nov. is proposed. The type strain is M-M1T ( = KCTC 23343T = CCUG 60020T).</jats:p
Marinomonas hwangdonensis sp. nov., isolated from seawater
No full textA Gram-negative, motile, rod-shaped bacterial strain, designated HDW-15T, was isolated from seawater of the Yellow Sea, Korea, and subjected to a polyphasic taxonomic study. Strain HDW-15T grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain HDW-15T fell within the clade comprising
Marinomonas
species, joining the type strain of
Marinomonas arctica
, with which it exhibited highest 16S rRNA gene sequence similarity (97.7 %). The 16S rRNA gene sequence similarity values between strain HDW-15T and the type strains of other
Marinomonas
species were in the range 93.7–97.2 %. Mean DNA–DNA relatedness values between strain HDW-15T and the type strains of
M. arctica
,
Marinomonas polaris
and
Marinomonas pontica
were 5.0–9.9 %. The DNA G+C content of the isolate was 48.7 mol%. Strain HDW-15T contained Q-8 as the predominant ubiquinone and C18 : 1ω7c, summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) and C16 : 0 as the major fatty acids. The major polar lipids found in strain HDW-15T were phosphatidylglycerol and phosphatidylethanolamine. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, showed that strain HDW-15T can be differentiated from other
Marinomonas
species. On the basis of the data presented, strain HDW-15T is considered to represent a novel species of the genus
Marinomonas
, for which the name Marinomonas hwangdonensis sp. nov. is proposed. The type strain is HDW-15T ( = KCTC 23661T = CCUG 61321T).</jats:p
Pedobacter insulae sp. nov., isolated from soil
No full textA Gram-negative, non-motile, rod-shaped bacterium, DS-139T, was isolated from a soil sample collected from Dokdo, Korea, and subjected to a polyphasic taxonomic analysis. Strain DS-139T grow optimally at 25 °C and pH 6.5-7.5 in the presence of 0-0.5% (w/v) NaCl. It contained MK-7 as the predominant menaquinone and iso-C15:0, C16:1ω7c and/or iso-C15:0 2-OH and iso-C17:0 3-OH as the major fatty acids. The DNA G + C content was 39.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DS-39T belongs to the genus Pedobacter in the family Sphingobacteriaceae. The similarity values between the 16S rRNA gene sequence of strain DS-139T and those of the type strains of recognized Pedobacter species, except Pedobacter saltans, were in the range 93.9-96.7%. The differential phenotypic properties, together with the phylogenetic distinctiveness, were sufficient to assign strain DS-139T to a species that is separate from recognized Pedobacter species. On the basis of the phenotypic and phylogenetic data, therefore, strain DS-139T represents a novel species of the genus Pedobacter, for which the name Pedobacter insulae sp. nov. is proposed. The type strain is DS-139T (=KCTC 12820T =DSM 18684T).open
Lutibacter aestuarii sp. nov., isolated from a tidal flat sediment, and emended description of the genus Lutibacter Choi and Cho 2006
No full textA Gram-negative, non-spore-forming, non-motile rod, designated MA-My1T, was isolated from a tidal flat sediment of the South Sea, Korea. Strain MA-My1T grew optimally at pH 7.0–7.5, at 30 °C and with 2.0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MA-My1T clustered with Lutibacter litoralis CL-TF09T and Lutibacter maritimus S7-2T, with which it exhibited 97.3 and 95.3 % 16S rRNA gene sequence similarity, respectively. Strain MA-My1T contained MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 0 3-OH and iso-C16 : 0 3-OH as the major fatty acids. The major polar lipids were phosphatidylethanolamine and two unidentified lipids. The DNA G+C content was 30.6 mol% and DNA–DNA relatedness between strain MA-My1T and L. litoralis JCM 13034T was 6.3±0.8 %. The differential phenotypic properties, together with phylogenetic and genotypic distinctiveness, distinguished strain MA-My1T from the members of the genus Lutibacter. On the basis of the data presented, strain MA-My1T is considered to represent a novel species of the genus Lutibacter, for which the name Lutibacter aestuarii sp. nov. is proposed. The type strain is MA-My1T ( = KCTC 23499T = CCUG 60022T).</jats:p
Preservation of salted Korean cabbage by inoculating halophilic lactic acid bacteria
No full textopen
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