194,329 research outputs found
TA-proteoliposome production.
No full textThe Syb2Cytb5OPG2 chimera was engineered to incorporate short peptide motifs immediately following the thrombin cleavage site at the N-terminus and a C-terminal opsin tag (OPG2) for the purpose of immunoblotting (A). Syb2Cytb5 (TA) was incubated with liposomes containing carboxyfluorescein (CF) and purified by size exclusion chromatography. The elution profile of the fluorescent cargo (B) was compared to that of the TA protein, detected by immunoblotting (C). The elution profile of free TA protein was analysed for comparison (D).</p
TA-proteoliposome characterisation.
No full textLiposome morphology was unaltered upon TA protein modification as imaged by transmission electron microscopy (A and B). Scale bar = 500nm. CF cargo in liposomes appears to be unaffected by TA incorporation (C): Total absorbance of the Dox-cargo was measured for the different preparations as indicated. The values represent the mean absorbance ± standard deviation (n = 3), and are normalised to the signal from protein-free liposomes (n = 1). (D): Total fluorescence from the CF cargo measured for the separate preparations. The values are shown as the mean fluorescence ± standard deviation (n = 3) and normalised to the mean protein-free liposome signal (n = 1). The Dox-cargo appears to be substantially retained upon TA modification.</p
Enhanced electron screening in d(d,p)t for deuterated Ta*
No full textThe recent observation of a large electron screening
effect in the d(d, p)t reaction using a deuterated Ta target has been
confirmed using somewhat different experimental approaches: for the electron screening potential energy. The high
value arises from the environment of the deuterons in the Ta
matrix, but a quantitative explanation is missing
Enhanced electron screening in d(d, p)t for deuterated Ta
No full textThe recent observation of a large electron screening effect in the d(d, p)t reaction using a deuterated Ta target has been confirmed using somewhat different experimental approaches: U-e = 309 +/- 12 eV for the electron screening potential energy. The high U, value arises from the environment of the deuterons in the Ta matrix, but a quantitative explanation is missing
Ta Moko: Culture, body modification, and the psychology of identity.
No full textThis paper outlines the context of Ta Moko in the Māori world, and locates the practice in the Pacific, and in the twenty first century. It describes the resurgence of the practice, and comments on the aims of the Marsen project. The three principal aims are:
1. To complete a comprehensive survey of the chant record and oral history with reference to archaeological, archival and artefactual materials.
2. To examine traditional whakairo carving in relation to Ta Moko.
3. To explore the nature of social relationships and ecologies that are supportive of, or resistant, to contemporary Ta Moko
Contemporary attitudes to traditional facial ta moko: A working paper
No full textUntil it came under serious attack from nineteenth century missionaries, ta moko was an
integral part of traditional Maori society. Facial moko conveyed important information about
identity, whakapapa and status. The process of receiving a moko was tapu and highly regulated.
Recent years have seen an increase in the number of Maori receiving ta moko. Moko have been
seen as a symbol of Maori pride and identity and have often been associated with political
activism. This study set out to investigate the contemporary meaning of ta moko, the reactions
wearers encounter from others and the ways wearers cope with those reactions.
Three case studies are presented. These show that the issues of personal identity and
whakapapa were central to the meaning wearers attached to their moko. Receiving a moko was
often associated with significant personal changes and an increased political commitment to
Maori self determination. On the whole, positive reactions were more common than negative
reactions but wearers did find themselves subjected to racist and antagonistic responses.
Wearing moko was also reported to mean that others, particularly other Maori, placed certain
expectations on the wearer, notably to be fluent in te reo and to be able to exercise leadership.
Participants considered that there was a need for education about the significance of ta moko
and recommended that those contemplating receiving a moko ensure that they are reasonably
fluent in te reo
Reliability, validity, and responsiveness of the Locomotor Capabilities Index in adults with lower-limb amputation undergoing prosthetic training
No full textFranchignoni F, Orlandini D, Ferriero G, Moscato TA. Reliability, validity, and responsiveness of the Locomotor Capabilities Index in adults with lower-limb amputation undergoing prosthetic training. Arch Phys Med Rehabil 2004;85:743-8. Objective To assess the reliability, validity, and responsiveness of both the standard and revised Locomotor Capabilities Index (LCI) in people with lower-limb amputation who undergo prosthetic training. Design Reliability and validity study. Setting Two freestanding rehabilitation centers. Participants Fifty inpatients with a recent unilateral lower-limb amputation. Interventions Not applicable. Main outcome measures The standard LCI and a new version with a 5-level ordinal scale (LCI-5) were tested for internal consistency, test-retest reliability, ceiling effect, and effect size. The construct validity of both versions was analyzed by correlation with the Rivermead Mobility Index, a timed walking test, and the FIM instrument. Results The Cronbach α of both LCI versions was .95. The item-to-total correlations (Spearman ρ) ranged from .50 to .87 (P<.0001 for all). The percent agreement and κ values for the item scores ranged, respectively, from 78.4% to 100% and .58 to 1.00 in the LCI, and from 75.7% to 97.3% and .54 to .96 in the LCI-5. The intraclass correlation coefficient (model 2,1) for the total scores was .98 for both versions; the Bland-Altman plot revealed no systematic trend for either version. Both the LCI and LCI-5 correlated with all criterion measures (ρ range, .61-.76), with the LCI-5 showing a larger effect size during the rehabilitation period and a lower ceiling effect. Patients with transtibial amputation were more independent in performing activities than were those with transfemoral amputation; their locomotor capability negatively correlated with age. Conclusions Both the LCI and LCI-5 captured the global locomotor ability of people with lower-limb amputation during prosthetic training. The new LCI-5 presents similar and sometimes better psychometric properties than the standard LCI
Innu ta’ ferħ biex jitkanta fil-proċessjoni ta’ nhar il-Lunzjata
No full textĠabra ta’ poeżiji u proża li tinkludi: Heros ta’ Dun Karm – Nocturne ta’ Ġużè Chetcuti – Lil Malta ta’ Dun Karm – Il-Ħjiena ta’ Ben Jussuf ta’ D. – Leħen il-Għaqal ta’ C. M. D. – Innu ta’ ferħ biex jitkanta fil-proċessjoni ta’ nhar il-Lunzjata ta’ Dun Karm.N/
TA-protein insertion into Dox-containing liposomes is saturable.
No full textTA-protein (Syb2Cytb5) in buffer containing octyl β-D-glucopyranoside (OGP) was incubated with liposomes at the specified concentrations. After incubation, the reactions were loaded onto a SEC column for purification. The fractions were collected and protein content was detected by immunoblotting for the C-terminal OPG2 epitope.</p
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