1,721,091 research outputs found
Allele frequencies for six STR loci (D5S818, D7S820, D8S1179, D13S317, D16S539, FGA) in a population sample of North Italy
No full textAllele frequencies for six STR loci (D5S818, D7S820, D8S1179, D13S317, D16S539, FGA) in a population sample of North Ital
Resizing reaction volumes for the ForenSeqTM DNA Signature Prep kit library preparation
No full textThe introduction of next generation sequencing (NGS; also known as massively parallel sequencing) technology in the field of forensic genetics has been welcomed by the scientific community, above all because it complements the weaknesses of capillary electrophoresis (CE) in the analysis of genetic markers, such as single nucleotide polymorphism (SNP) typing. However, one of the main obstacles to its adoption does not seem to be the cost of the instrumentation, but rather the cost of the NGS library preparation kits. With the aim of reducing the cost of library preparation without compromising the quality of the results, we tried to scale down reaction volumes for the first two polymerase chain reactions in the amplification and enrichment phases of the targeted loci of library preparation using the ForenSeq DNA Signature Prep kit. We used 1 L templated DNA input to a concentration of 1 ng/L, instead of the 5 L at 0.2ng/L recommended by the manufacturer. Our findings indicate that reduction of the library preparation volume using the ForenSeq DNA Signature Prep kit did not interfere with the quality and reproducibility of the DNA profiles obtained and can help lower the overall cost of NGS
Population data of three X chromosomal STRs: DXS7132, DXS7133 and GATA172D05 in North Italy.
No full textPopulation genetic comparisons of three X-chromosomal STRs (DXS7132, DXS7133 and GATA172D05) in North and South Italy.
No full textPrime riflessioni medico legali sul nuovo ordinamento della RC sanitaria (legge 8 marzo 2017, n. 24, Gelli-Bianco)
No full textLa legge Gelli-Bianco pone sicuramente in risalto il contributo della medicina legale nella valutazione e definizione degli eventuali profili di responsabilità emergenti nel corso dell’attività sanitaria
Due singolari casi di morte da avvelenamento combinato di monossido di carbonio e cianuro.
No full textVengono presentati due singolari casi di morte per avvelenamento combinato di cianuri e monossido di carboni
Is the stamp son of the acoustic prothesis?
No full textWe describe an unusual case of deficient paternity testing, requested by the heirs, the wife and two legitimate daughters, on a father and his putative son, deceased respectively in 2007 and 2011. The analysis was resolved on two personal items of the defuncted: a postcard stamp 17 years-old, presumably sent by the putative son, and an acoustic prothesis belonged to the alleged father. Twenty-two autosomal short tandem repeats (STRs) from the commercial kit Powerplex® Fusion kit (Promega, Madison, WI) were analyzed on the acoustic prothesis and the mother–daughters trio, giving a probability of paternity (W) of 99.999% and therefore confirming that the father owned the prothesis. Analysis of both autosomal and Y-chromosomal STRs by means of Powerplex® Fusion System and Powerplex® Y23 System (Promega, Madison, WI) was carried out on the two personal belongings.The obtained probability of paternity value W of 99.998% from autosomal STRs and the presence of an identical Y-chromosomal haplotype shared between the two males confirmed the genetic compatibility of the alleged father and his son
Optimization of DNA extraction and typing from skeletal remains.
No full textOptimization of DNA extraction and typing from skeletal remains
Evaluation of different STR typing kits on DNA extracted from fingernails of unidentified decomposed cadavers
No full textEvaluation of different STR typing kits on DNA extracted from fingernails of unidentified decomposed cadaver
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