741 research outputs found
A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textMassa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto, Verde, Gabriella Lo (2023): A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation. Zootaxa 5270 (3): 351-400, DOI: 10.11646/zootaxa.5270.3.1, URL: http://dx.doi.org/10.11646/zootaxa.5270.3.
FIGURE 5 in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 5. Male titillators of different taxa/populations of the genus Roeseliana.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 368, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
FIGURE 8 in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 8. Ventral view of the female subgenital plate of six taxa of Roeseliana.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 371, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
High-Performance PCR for Alleles Discrimination of Chromo-Helicase-DNA Binding Protein (CHD1) Gene in Bird Sexing
No full textGenetic analyses aiming at assessing the presence of specific sequences or alleles are often carried out by PCR. Sexing of most birds is nowadays based on PCR with “universal” primers and relies on the assessment of the presence of the sex-linked CHD1-Z and -W alleles. The entire workflow is relatively time-consuming, especially for batch analyses, whereas methods that allow carrying out the entire procedure in a short time are highly desirable. The only method for outdoor analyses reported so far relies on LAMP; however; it fails to work properly in Procellariiformes. Besides improving the LAMP test; we have developed a PCR-based DNA amplification procedure (named high-performance PCR); whose unique features allow it to outperform standard PCR; making possible the direct, in-tube visual reading of results. We tested it with specifically designed Procellariiformes-targeted primer sets for rapid sexing of the birds using fluorimetric detection. The protocol, combined with rapid DNA extraction, allows for fast reading of results without electrophoresis within less than 1 h from sampling. The technique could be extended to other species, as well as to many other applications
FIGURE 12 in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 12. Roeseliana ambitiosa from Bulgaria, calling song: (a), Sound Pressure Envelope, 5 s; (b), Sound Pressure Envelope, 5 s; (c), Pulses (tooth strikes), one syllable; (d), Time/Frequency Spectrogram, 6 s; (e), Frequency/Sound Pressure analysis, spot in one syllable.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 376, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
FIGURE 20 in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 20. Syllable comparison: (a), ambitiosa from Bulgaria; (b), ambitiosa from Greece; (c), azami minor from Switzerland; (d) azami from Cote d'Azur, France; (e), brunneri from Valli di Chioggia, Italy; (f), brunneri from Valle dell'Ortazzo, Italy; (g), roeselii from Alps, Italy; (h), roeselii from Netherlands.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 384, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
FIGURE 15. Roeseliana azami from Cote d in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 15. Roeseliana azami from Cote d'Azur, calling song: (a), Sound Pressure Envelope, 5 s; (b), Sound Pressure Envelope, 0.25 s; (c), Pulses (tooth strikes), one syllable; (d), Time/Frequency Spectrogram, 4 s; (e), Frequency/Sound Pressure analysis, spot in one syllable.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 379, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
FIGURE 13 in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 13. Roeseliana ambitiosa from Greece, calling song: (a), Sound Pressure Envelope, 5 s; (b), Sound Pressure Envelope, 0.25 s; (c), Pulses (tooth strikes), one syllable; (d), Time/Frequency Spectrogram, 5 s; (e), Frequency/Sound Pressure analysis, spot in one syllable.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 377, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
FIGURE 16 in A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation
No full textFIGURE 16. Roeseliana brunneri from Valli di Chioggia, calling song: (a), Sound Pressure Envelope, 5 s; (b), Sound Pressure Envelope, 0.2 s; (c), Pulses (tooth strikes), one syllable; (d), Time/Frequency Spectrogram, 6 s; (e), Frequency/Sound Pressure analysis, spot in one syllable.Published as part of Massa, Bruno, Tagliavia, Marcello, Buzzetti, Filippo Maria, Fontana, Paolo, Carotti, Giovanni, Bardiani, Marco, Leandri, Fausto, Scherini, Roberto & Verde, Gabriella Lo, 2023, A taxonomic revision of the Palaearctic genus Roeseliana (Orthoptera: Tettigoniidae: Tettigoniinae: Platycleidini): a case of ongoing Mediterranean speciation, pp. 351-400 in Zootaxa 5270 (3) on page 380, DOI: 10.11646/zootaxa.5270.3.1, http://zenodo.org/record/785988
Complete decontamination and regeneration of DNA purification silica colum
No full textSilica columns are among the most used DNA purification systems, allowing a good yield of high-quality nucleic acids without organic extractions. Silica column regeneration protocols reported up to now to
remove DNA traces are time-consuming, and their effectiveness on genomic DNA has not been demonstrated. Here we report a very rapid regeneration procedure that ensures no DNA carryover, independent
of its size, without impairing column efficiency. The method takes advantage of the improved DNA removal by low concentrations of Triton
X-100
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