170,128 research outputs found
Tessuto Nervoso
No full textMedical histology
Editor / s
Nadir M. Maraldi, Carlo Tacchetti
Author / s
Saverio Cinti, Michelangelo Cordenonsi, Simona Corso, Ottavio Cremona, Massimo De Felici, Roberta Di Pietro, Nicoletta Gagliano, Silvia Giordano, Nadir M. Maraldi, Carla Martinelli, Alessandro Moretta, Sergio Morini, Beatrice Nico, Maria Prat, Domenico Puzzolo, Domenico Ribatti, Giovanni Francesco Spatola, Carlo Tacchetti
ISBN
9788870513899
Medical histology
Medical histology is a modern text more oriented in the "medical" sense, in order to provide students with the tools to frame the subject in the context of preclinical disciplines. In it are presented experimentally acquired data, framed in mechanistic models whose validity is widely confirmed and which contribute to an understanding of the pathophysiological processes. The goal was to provide a model of study of a complex subject, which is also applicable to other disciplines that the student will meet in the course of his medical studies.
The volume has been divided into blocks of teaching material:
• text and figures - essential subjects;
• in-depth analysis with figures - supplementary topics;
• areas of clinical histology - physiopathological correlations;
• extra content in the Virtual Campus web site - additional material available in multimedia format.
Each chapter contains a paragraph concerning the Prerequisites, that is the basic knowledge necessary for a correct classification of the topics covered, a paragraph concerning the Objectives that the study of the topic is aimed at and a Summary of the topic dealt with and concludes with Concepts key to the topics covered.
The topics have been developed highlighting the differentiation mechanisms that allow, in both embryonic and adult life, the exchange and integration of stem cell elements in the context of functional specialized populations. Particular attention was paid to the biomechanical properties of tissues and to the properties of the different populations of adult stem cells, also in consideration of their increasing use, also in association with biomaterials, in regenerative medicine
Cartilage in ageing and osteoarthritis: altered expression of fibronectin and related integrin receptors
No full textCartilage in ageing and osteoarthritis: altered expression of fibronectin and related integrin receptors
No full text
Advanced correlative light/electron microscopy: current methods and new developments using Tokuyasu cryosections
No full textMicroscopy is an essential tool for analysis of cellular structures and function. With the advent of new fluorescent probes and super-resolution light microscopy techniques, the study of dynamic processes in living cells has been greatly facilitated. Fluorescence light microscopy provides analytical, quantitative, and three-dimensional (3D) data with emphasis on analysis of live cells using fluorescent markers. Sample preparation is easy and relatively inexpensive, and the use of appropriate tags provides the ability to track specific proteins of interest. Of course, only electron microscopy (EM) achieves the highest definition in terms of ultrastructure and protein labeling. To fill the gap between light microscopy and EM, correlative light and electron microscopy (CLEM) strategies have been developed. In particular, hybrid techniques based upon immuno-EM provide sensitive protein detection combined with high-resolution information on cell structures and protein localization. By adding the third dimension to EM with electron tomography (ET) combined with rapid freezing, CLEM techniques now provide additional tools for quantitative 3D analysis. Here, we overview the major methods applied and highlight the latest advances in the field of CLEM. We then focus on two selected techniques that use cryosections as substrate for combined biomolecular imaging. Finally, we provide a perspective of future developments in the field
Cartilage in ageing and osteoarthritis: altered expression of fibronectin and related integrin receptors.
No full text
Immunogold Electron Microscopy of the Autophagosome Marker LC3
No full textEven though autophagy was firstly observed by transmission electron microscopy already in the 1950s (reviewed in Eskelinen et al., 2011), nowadays this technique remains one of the most powerful systems to monitor autophagic processes. The autophagosome, an LC3-positive double membrane structures enclosing cellular materials, represents the key organelle in autophagy and its simple visualization and/or numeration allow to draw important conclusions about the autophagic flux. Therefore, the accurate identification of autophagosomes is crucial for a comprehensive and detailed dissection of autophagy. Here we present a simple protocol to identify autophagosomes by transmission electron microscopy coupled to immunogold labeling of LC3 starting from a relatively low cell number, which we recently developed to follow the autophagic pathway during viral-mediated human carcinogenesis
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