1,043 research outputs found
Effetto di polimorfismi genetici coinvolti nel processo infiammatorio e nel danno ossidativo nella malattia di Alzheimer
No full textDott. ssa Eliana Venturelli Dottorato in Medicina Molecolare, Ciclo XIX TITOLO: Effetto di polimorfismi genetici coinvolti nel processo infiammatorio e nel danno ossidativo nella malattia di Alzheimer Alzheimer s Disease (AD) is a multifactorial disease caused by both genetic and environmental factors. The aim of this project was to carry out association studies, to detect significant differences in polymorphism frequency in molecules involved in AD pathogenesis, including Nitric Oxide Synthase (NOS) isoforms (NOS3 and NOS1) and IP-10 (CXCL10) gene between patients and age matched healthy controls. ApoE, NOS3 Glu298Asp and T-786C, NOS1 C276T, VNTR and G-84A, were determined by PCR-RFLP and IP-10 exons were sequenced. ApoE e4 allele, NOS3 wild type (Glu/Glu) genotype, NOS1 T/T genotype and VNTR short alleles have been demonstrated to act as a risk factor for AD. On the contrary NOS3 T-786C, NOS1 G-84A and IP-10 did not increase the risk to develop the pathology. In conclusion, our genetic analysis contributed to identify some susceptibility factors for AD. Future perspectives are represented by the possibility to carry out pharmacogenetic studies for identifying responders and non responders
Influenze deweyane nella pratica didattica di un maestro nell’Italia del secondo dopoguerra
No full textIl pensiero di Dewey ha attraversato la pedagogia del secondo Novecento influenzando profondamente la storia educativa e scolastica italiana. Il presente contributo, attraverso la prospettiva delle biografie magistrali, intende provare a rintracciare come la lezione deweyana sia stata effettivamente parte della formazione di maestri e maestre nell’Italia del secondo dopoguerra e poi elaborata all’interno della pratica didattica quotidiana
Immunomodulating activity of heparin: evaluation of structure and physico-chemical properties of heparin
No full textImmunomodulating activity of heparin: evaluation of structure and physico-chemical properties of hepari
Activity of chondroitin ABC lyase and hyaluronidase on free-radical degraded chondroitin sulfate
No full textHigh molecular mass-chondroitin sulfate was characterized for M(r), charge density and constituent disaccharides. This glycosaminoglycan was depolymerized by a controlled free-radical process mediated by hydrogen peroxide in the absence or presence of cupric or ferrous ions. Hydrogen peroxide depolymerizes chondroitin sulfate, and the velocity of the reaction increases in the presence of cupric ions and, further, of ferrous ions. Different low molecular mass-chondroitin sulfate fractions were produced and analyzed by high-performance size-exclusion chromatography and polyacrylamide-gel electrophoresis. This last technique strongly supports the hypothesis that the free-radical process proceeds by the destruction of disaccharide units. The treatment of free-radical chondroitin sulfate samples with chondroitinase ABC and testicular hyaluronidase results in a lower capacity of these enzymes to degrade these glycosaminoglycan derivatives with respect to the natural sample. This was confirmed by polyacrylamide-gel electrophoresis and by the time-courses of enzymatic treatment evaluated by spectrophotometric technique (for treatment with chondroitin ABC lyase)
QUALITATIVE AND QUANTITATIVE STUDIES OF HEPARIN AND CHONDROITIN SULFATES IN NORMAL HUMAN PLASMA
No full textHeparin was extracted and purified from normal human plasma, and full characterization of its structure and physico-chemical properties was achieved for the first time. Plasma was submitted to exhaustive proteolytic treatment with papain, trypsin, chymotrypsin, collagenase and pepsin, anion-exchange chromatography and precipitation with organic solvents. By this procedure, we recovered heparin (about 0.7 mg/100 ml of plasma) and chondroitin sulfate (about 0.1 mg/100 ml of plasma). Chondroitin sulfate has a peak molecular mass of about 15 630, and it is composed of about 60% nonsulfated disaccharide, 3.5% disaccharide 6-monosulfate and about 40% disaccharide 4-monosulfate, with a sulfate-to-carboxyl ratio of 0.41. Heparin, identified by agarose-gel electrophoresis, is constituted by about 40% slow-moving component and about 60% fast-moving species. This glycosaminoglycan had a peak molecular mass of about 7000, and was identified as 'typical' heparin by its constituent disaccharide composition. About 70% of disaccharides were identified as trisulfated disaccharide, and about 18% as disulfated disaccharides, 3% as monosulfated disaccharides and 10% as nonsulfated disaccharide. Heparin extracted from normal human plasma has a high sulfate-to-carboxyl ratio (2.47) and in vitro anticoagulant activity of about 70 I.U. A more quantitative and statistical analysis performed on 10 ml of plasma obtained from 10 human healthy volunteers revealed a heparin level of 0.54 +/- 0.17 mg/100 ml plasma (mean +/- standard deviation) with a coefficient of variation of about +/- 32%. These findings demonstrate for the first time the presence of heparin molecules in normal human plasma and confirm the importance of adequate extraction processes to purify a molecule that strongly interacts with plasma protein components. This is discussed in light of other authors that described a polysaccharide molecule named heparan sulfate in human plasma
activity of chondroitin ABC lyase on dermatan sulfate partially degraded by cupric-ion-mediated free-radical treatment
No full textDermatan sulfate was extracted and purified from bovine intestinal mucosa, pig intestinal mucosa and pigskin. Small differences in M(r), charge density and constituent disaccharides were detected for the three purified natural dermatan sulfates. Bovine intestinal mucosa dermatan sulfate was depolymerized by a controlled free-radical process mediated by cupric ions in the presence of hydrogen peroxide. Different low-molecular-mass dermatan sulfate fractions were produced and analysed by high-performance size-exclusion chromatography and polyacrylamide gel electrophoresis. The results obtained by this last technique strongly support the hypothesis that the free-radical process proceeds essentially via the destruction of disaccharide units. The partial degradation of dermatan sulfates by cupric-ion-mediated free-radical treatment reduces or even eliminates the capacity of chondroitin ABC lyase to depolymerize these derivatives. This was confirmed by polyacrylamide gel electrophoresis and the time curves of enzymatic treatments evaluated by spectrophotometry
Nimrod e Babele
No full textLeitmotif of this paper is the “eterogenesi dei fini”, considered as one of the most important theme of philosophy and of theology of history; general symbols of it are myths of Nimrod and Babel: on the one hand we can take into consideration the great universal man, such as a super-human hero and a God’s enemy, but on the other hand we have to regard the big city, place of work and scene of political collective consent and of totalitarian state organization.
This essay aims to give a wider philosophical interpretation of Genesis Tales, focusing on Abraham’s life, considering him as an archetype of freedom and of personal responsability.
According to last empirical meaning of this paper, the author is going to adopt a new theoretical perspective by lending importance to the basic question about intelligibiliy of human history
Imagen computacional: reseña sobre la imagen como simulación
No full text[EN] This article is a bibliographic review about an image issue in the computational art creation environment. With the technological
breakthroughs, specially in the computer graphics field, computational images insert themselves in the art field to understand and
discuss how they can interfere in sign and meaning matters. With computational technology, images became machine generated and
this phenomenon’s impacts are still under investigation. This work is based on the discussions brought by Edmond Couchot, Oliver
Grau, Cláudia Gianetti, José Luis Brea, Vilém Flusser, Suzete Venturelli and Cleomar Rocha, and we aim to present the aesthetics of the
current computational images.[ES] Este artículo es una revisión bibliográfica sobre un problema de imagen en el entorno de creación de arte computacional. Con los
avances tecnológicos, especialmente en el campo de los gráficos por computadora, las imágenes computacionales se insertan en el
campo del arte para comprender y discutir cómo pueden interferir en los asuntos de signos y significados. Con la tecnología
computacional, las imágenes se convirtieron en generadas por máquina y los impactos de este fenómeno aún están bajo investigación.
Este trabajo se basa en las discusiones presentadas por Edmond Couchot, Oliver Grau, Cláudia Gianetti, José Luis Brea, Vilém Flusser,
Suzete Venturelli y Cleomar Rocha, y nuestro objetivo es presentar la estética de las imágenes computacionales actuales.Da Silva, T.; Venturelli, S. (2019). Computational Image: a review about the image as simulation. En IV Congreso Internacional de investigación en artes visuales: ANIAV 2019 Imagen [N] visible. Editorial Universitat Politècnica de València. 184-188. https://doi.org/10.4995/ANIAV.2019.2019.8953OCS18418
Studio qualitativo e quantitativo di eparina e condroitin solfato in plasma umano non patologico
No full textStudio qualitativo e quantitativo di eparina e condroitin solfato in plasma umano non patologic
Separazione specifica e determinazione quantitativa del condroitin solfato in plasma umano normale
No full textAim of this study is to examine structure and physico-chemical properties of chondroitin sulfate in normal human plasma in relation to thè sex and age of human healthy volunteers. Chondroitin sulfate was purified from 15-20 mL of plasma of 17 male and 15 female. By agarose-gel electrophoresis and spectrophotometric analysis with DMB we recovered about 0.1 mg/100 mi of plasma chondroitin sulfate. No differences were detected between thè group of male and female donors and no significant relationship was determined between chondroitin sulfate concentration and age. This glycosaminoglycan has a peak molecular mass of about 15,OOO with a sulfate-to-carboxyl rado of about 0.55. This extraction and purification procedure and thè analytical techniques used to determine structure and physico-chemical properties of normal human plasma chondroitin sulfate can be adopted to evaluate possible differences in thè amount and primary structure of this glycosaminoglycan in plasma of patients suffering for particular diseases, such as degenerative and inflammatory conditions of articular cartilage. This may be useful in the diagnosis and follow up of therapy
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