2,613 research outputs found
Tergoceracris guajataca Perez-Gelabert & Otte, 2003, n. sp.
No full textTergoceracris guajataca n. sp. Figs. 3, 5 C, 6 A, 7, 8, 9, 14, 15 Diagnosis.— Similar in coloration and morphology to T. cerropunta and T. cayey while differentiated by the wing color from T. luquillensis. Differs from T. cerropunta and T. cayey as follows: Endophallic plate relatively shorter (about 1.3X as long as ventral valve of aedeagus) (Fig. 15 D); epiproct lateral ridges unilobed and offcenter mounds elongate (Fig. 6 A) (epiproct lateral ridges bilobed in T. cayey and offcenter mounds short in T. cerropunta); lophi of epiphallus more robust in dorsal view (Fig. 14 D). Description.— Male. Coloration very similar to that of T. cerropunta, both brightly adorned. Pronotum: annular area light greenish brown in center surrounded by black and cream yellow bands. Wide band at pronotal side green and light brown, followed below by arched cream yellow band that delimits smaller portion of green on lower margin. Wings: Tegmina short, pink reddish with some black on posterior edge, with surface markedly reticular. Abdomen: dorsal midline marked by interrupted black streak. Furculae vertically elevated, slightly longer and thinner than in the other Puerto Rican species, ascending jointly, narrowing, then separating and widening only slightly on upper portion. Internal genitalia: As illustrated in Figs. 14, 15. Etymology.— The name is derived from the type locality. Type material.— Holotype: Male. PUERTO RICO, Guajataca St. Forest, 7 miles SE Isabela, 600 ft. (200 m), 20.vi. 1969, T. J. Cohn (ANSP). Allotype: female. Same data as holotype (ANSP). Paratypes: One male, 2 females, same data as holotype (ANSP). Habitat.—Specimens were collected at about 600 ft.( 200 m) of elevation in the Guajataca State forest, near Isabela, Puerto Rico. This area is substantially lower than for the three other species. Vegetation there is mostly wet tropical forest. In a visit to this reserve and search around several trails by the senior author and Santo Navarro in October 2001, this forest was found to be rather dry and no specimens of these grasshoppers were encountered.Published as part of Perez-Gelabert, Daniel E. & Otte, Daniel, 2003, Tergoceracris, a new genus and six new species of montane grasshoppers (Orthoptera: Acrididae: Ommatolampinae) from Dominican Republic and Puerto Rico, pp. 1-31 in Zootaxa 155 on pages 6-7, DOI: 10.5281/zenodo.15697
GERDA datasets including NGS and SGA data
No full textDatasets linked to publication "Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods", Otte et al 2023 published in Cell Reports Methodspre-ART (antiretroviral therapy) cryo-conserved and and whole blood specimen were sampled for HIV-1 virus reservoir determination in HIV-1 positive individuals from the Swiss HIV Study Cohort. Patients were monitored for proviral (DNA), poly-A transcripts (RNA), late protein translation (Gag and Envelope reactivation co-detection assay, GERDA) and intact viruses (golden standard: viral outgrowth assay, VOA).In this dataset we deposited the pipeline for the multidimensional data analysis of our newly established GERDA method, using DBScan and tSNE. For further comprehension NGS and Sanger sequencing data were attached as processed and raw data (GenBank).Resubmitted to Cell Reports Methods (Jan-2023), accepted in principal (Mar-2023)GERDA is a new detection method to decipher the HIV-1 cellular reservoir in blood (tissue or any other specimen). It integrates HIV-1 Gag and Env co-detection along with cellular surface markers to reveal 1) what cells still contain HIV-1 translation competent virus and 2) which marker the respective infected cells express. The phenotypic marker repertoire of the cells allow to make predictions on potential homing and to assess the HIV-1 (tissue) reservoir.All FACS data were acquired on a LSRFortessa BD FACS machine (markers: CCR7, CD45RA, CD28, CD4, CD25, PD1, IntegrinB7, CLA, HIV-1 Env, HIV-1 Gag)Raw FACS data (pre-gated CD4CD3+ T-cells) were arcsin transformed and dimensionally reduced using optsne. Data was further clustered using DBSCAN and either individual clusters were further analyzed for individual marker expression or expression profiles of all relevant clusters were analyzed by heatmaps.Sequences before/after therapy initiation and during viral outgrowth cultures were monitored for individuals P01-46 and P04-56 by Next-generation sequencing (NGS of HIV-1 Envelope V3 loop only) and by Sanger (single genome amplification, SGA)data normalization code (by Julian Spagnuolo)FACS normalized data as CSV (XXX_arcsin.csv)OMIQ conText file (_OMIQ-context_XXX)arcsin normalized FACS data after optsne dimension reduction with OMIQ.ai as CSV file (XXXarcsin.csv.csv)R pipeline with codes (XXX_commented.R)P01_46-NGS and Sanger sequencesP04_56-NGS and Sanger sequence
GERDA datasets including NGS and SGA data
No full textDatasets linked to publication "Revealing viral and cellular dynamics of HIV-1 at the single-cell level during early treatment periods", Otte et al 2023 published in Cell Reports Methodspre-ART (antiretroviral therapy) cryo-conserved and and whole blood specimen were sampled for HIV-1 virus reservoir determination in HIV-1 positive individuals from the Swiss HIV Study Cohort. Patients were monitored for proviral (DNA), poly-A transcripts (RNA), late protein translation (Gag and Envelope reactivation co-detection assay, GERDA) and intact viruses (golden standard: viral outgrowth assay, VOA).In this dataset we deposited the pipeline for the multidimensional data analysis of our newly established GERDA method, using DBScan and tSNE. For further comprehension NGS and Sanger sequencing data were attached.Resubmitted to Cell Reports Methods (Jan-2023), accepted in principal (Mar-2023)GERDA is a new detection method to decipher the HIV-1 cellular reservoir in blood (tissue or any other specimen). It integrates HIV-1 Gag and Env co-detection along with cellular surface markers to reveal 1) what cells still contain HIV-1 translation competent virus and 2) which marker the respective infected cells express. The phenotypic marker repertoire of the cells allow to make predictions on potential homing and to assess the HIV-1 (tissue) reservoir.All FACS data were acquired on a LSRFortessa BD FACS machine (markers: CCR7, CD45RA, CD28, CD4, CD25, PD1, IntegrinB7, CLA, HIV-1 Env, HIV-1 Gag)Raw FACS data (pre-gated CD4CD3+ T-cells) were arcsin transformed and dimensionally reduced using optsne. Data was further clustered using DBSCAN and either individual clusters were further analyzed for individual marker expression or expression profiles of all relevant clusters were analyzed by heatmaps.Sequences before/after therapy initiation and during viral outgrowth cultures were monitored for individuals P01-46 and P04-56 by Next-generation sequencing (NGS of HIV-1 Envelope V3 loop only) and by Sanger (single genome amplification, SGA)data normalization code (by Julian Spagnuolo)FACS normalized data as CSV (XXX_arcsin.csv)OMIQ conText file (_OMIQ-context_XXX)arcsin normalized FACS data after optsne dimension reduction with OMIQ.ai as CSV file (XXXarcsin.csv.csv)R pipeline with codes (XXX_commented.R)P01_46-NGS and Sanger sequencesP04_56-NGS and Sanger sequence
Coherent dynamics of atomic spins on a surface
No full textThis thesis presents experiments on the free, coherent spin dynamics ofmagnetic nanostructures built out of individual titanium atoms adsorbed on bilayer MgOislands grown on a Ag(100) crystal inside a scanning tunneling microscope (STM).We use the exchange field of the spin-polarized probe tip to tune the eigenstates of the magnetic structures to superpositions of the Zeeman product states. These superpositions appear as avoided level crossings of the eigenenergies as a function of tip-atom distance and are detected through electron spin resonance (ESR) measurements. Subsequently,we put the STMtip at the position where we detect an avoided crossing and we perform a DC pump-probe experiment. Here, the pump pulse causes a single spin flip on the atom under state tip which is then allowed to evolve freely for some time t until it is measured by the subsequent probe pulse. By varying the time t between pump and probe over many experiments, we are able to trace out the evolution of the spin under the tip with nanosecond time resolution....QN/Otte La
Compte rendu de l'ouvrage Handbuch zur Geschichte Südosteuropas, t. 1, Herrschaft und Politik in Südosteuropa von der Römischen Antike bis 1300, éd. Oliver Jens Schmitt, Peter Schreiner, Fritz Mitthof, Berlin–Boston, De Gruyter Oldenbourg, 2019 ; 2 vol., 1 121 p. (Reference). ISBN : 978-3-11-063966-7. Prix : € 189,95.
No full textCompte rendu de l'ouvrage suivant: Handbuch zur Geschichte Südosteuropas, t. 1, Herrschaft und Politik in Südosteuropa von der Römischen Antike bis 1300, éd. Oliver Jens Schmitt, Peter Schreiner, Fritz Mitthof, Berlin–Boston, De Gruyter Oldenbourg, 2019 ; 2 vol., 1 121 p. (Reference). ISBN : 978-3-11-063966-7. Prix : € 189,95. Compte rendu rédigé en anglais par Davor Salihović et traduit en français par Sandra Otte
Tergoceracris cayey Perez-Gelabert & Otte, 2003, n. sp.
No full textTergoceracris cayey n. sp. Figs. 3, 5 E, 6 B, 9, 18, 19 Diagnosis.— Distinguished from guajataca as follows: endophallic plate relatively longer (1.41.5X as long as ventral valve) (Fig. 18 D); epiproct lateral ridges bilobed and offcenter mounds longer and somewhat bilobed (Fig. 6 B). Differs from cerropunta as follows: furculae with wide waist (Fig. 5 E); offcenter mounts of epiproct elongate; ventral valve of epiphallus thin (Fig. 19 F). Description.— Male. General coloration bright green. Size of unique individual rather large, similar in this way to males of T. guajataca. Pronotum as in other species in the genus dorsally bearing dark annular ring surrounded by yellow. Wings: Reddish pink and rounded, nonoverlapping and about the same length as pronotum. Legs: Brilliant green and robust, especially the hind femora. Abdomen: Abdominal segments marked by wide and interrupted black streak on dorsum. Furculae vertically elevated, rather narrow and not flaring at all on top. Their shape intermediate between those of T. guajataca and T. cerropunta on the one hand and T. luquillensis on the other. Internal genitalia as in Figs. 18, 19. Etymology.— The name is derived from the type locality. Type material.— Holotype: Male, PUERTO RICO, Tetas de Cayey, near top of left tit, 23.x. 2001, D. E. PerezGelabert (NMNH). Habitat.— Tetas de Cayey are a pair of rocky outcrops of volcanic origin located on the south central portion of Puerto Rico. Their elevation nears one thousand meters and they are mostly covered with cloud forest vegetation. The shrubs and trees on the hillside were exhaustively searched for over three hours finding only a single specimen.Published as part of Perez-Gelabert, Daniel E. & Otte, Daniel, 2003, Tergoceracris, a new genus and six new species of montane grasshoppers (Orthoptera: Acrididae: Ommatolampinae) from Dominican Republic and Puerto Rico, pp. 1-31 in Zootaxa 155 on page 8, DOI: 10.5281/zenodo.15697
‘“The Diplomatic Digestive Organ”':The Foreign Office as the Nerve Centre of Foreign Policy, c. 1800-1940
No full textForeign ministries form a central part of modern diplomatic practice. They emerged slowly and haphazardly from the late fifteenth century onwards. With the growth in scope – both geographical and temporal – and intensity of diplomacy came the need for a central organization that could control and coordinate policy at the seat of government. In Tudor and Elizabethan England, too, the steady growth of diplomatic activity spurred on institutional change in the shape of the Principal Secretary of State. Initially, an officer of the royal household, executing the decisions of the monarch and the Privy Council, over time much of his business came to be focused on foreign affairs
Truncotettix Perez-Gelabert, Hierro and Otte 1998
No full textTruncotettix Perez-Gelabert, Hierro and Otte, 1998 Fig. 17−18 Perez-Gelabert et al. (1998) described this genus with two species: T. fronterizus Perez-Gelabert, Hierro and Otte, 1998 (Fig. 17) and T. interruptus Perez-Gelabert, Hierro and Otte, 1998 (Fig. 18), both from Dominican Republic. This genus has a relatively very small body size among Neotropical cladonotines, and 12-segmented antennae and a tectiform pronotum with the anterior margin slightly produced over vertex in dorsal view (Perez-Gelabert et al. 1998; Fig. 17, 18). The two species are very similar, but can be distinguished by posterior margin of pronotum, which presents a notch in T. fronterizus (Fig. 17C) and is slightly truncated in T. interruptus (Fig. 18C).Published as part of Silva, Daniela Santos Martins, Cadena-Castañeda, Oscar J., Pereira, Marcelo Ribeiro, Domenico, Fernando Campos De & Sperber, Carlos Frankl, 2019, New tribes, overview and checklist of Neotropical Cladonotinae (Orthoptera: Caelifera: Tetrigidae), pp. 1-38 in Insecta Mundi 723 (723) on page 22, DOI: 10.5281/zenodo.367493
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