205 research outputs found
Massive NGS Data Analysis Reveals Hundreds Of Potential Novel Gene Fusions in Human Cell Lines
No full textBackground: gene fusions derive from chromosomal rearrangements. The resulting chimeric transcripts are often endowed
with oncogenic potential. Furthermore, they serve as diagnostic tools for the clinical classification of cancer subgroups with
different prognosis and, in some cases, they can provide specific drug targets. To date, many efforts have been carried out to
study gene fusion events occurring in tumor samples. In recent years, the availability of a comprehensive next-generation
sequencing dataset for all existing human tumor cell lines has provided the opportunity to further investigate these data in
order to identify novel and still uncharacterized gene fusion events. Results: In our work, we have extensively reanalyzed
935 paired-end RNA-sequencing experiments downloaded from the Cancer Cell Line Encyclopedia repository, aiming at
addressing novel putative cell-line specific gene fusion events in human malignancies. The bioinformatics analysis has
been performed by the execution of four gene fusion detection algorithms. The results have been further prioritized by
running a Bayesian classifier that makes an in silico validation. The collection of fusion events supported by all of the
predictive software results in a robust set of ∼1,700 in silico predicted novel candidates suitable for downstream analyses.
Given the huge amount of data and information produced, computational results have been systematized in a database
named LiGeA. The database can be browsed through a dynamic and interactive web portal, further integrated with
validated data from other well-known repositories. Taking advantage of the intuitive query forms, the users can easily
access, navigate, filter, and select the putative gene fusions for further validations and studies. They can also find suitable
experimental models for a given fusion of interest. Conclusions: We believe that the LiGeA resource can represent not only
the first compendium of both known and putative novel gene fusion events in the catalog of all of the human malignant
cell lines but it can also become a handy starting point for wet-lab biologists who wish to investigate novel cancer
biomarkers and specific drug targets
The ATRA-21 gene-expression model predicts retinoid sensitivity in CEBPA double mutant, t(8;21) and inv(16) AML patients
No full text"Nonostante la vostra cortese ospitalità...dalla chiusura dei manicomi alla tutela della salute mentale oggi"
No full textMostra promossa dalla Provincia di Bologna in collaborazione con il Dipartimento Salute Mentale ASL di Bologna in occasione del trentesimo anniversario della Legge 180 (Legge Basaglia).
La mostra ha proposto una selezione di materiali storici (fotografie, documenti, lettere) relativi al periodo subito precedente l'approvazione della Legge 180, unitamente ad una ricerca fotografica condotta oggi da 3 giovani autori laureati DAMS che hanno raccontato come viene attualmente affrontata, in strutture pubbliche, la gestione del disagio mentale.
La mostra è stata ospitata dal 19 Dicembre 2008 al 30 Gennaio 2009 negli spazi espositivi dell'Accademia di Belle Arti di Bologna.
Il catalogo relativo alla mostra è stato inserito in altra scheda dell'Anagraf
Effects of alfaxalone or propofol on giant-breed dog neonates viability during elective caesarean sections
No full textNowadays, thanks to the increased awareness of owners and breeders and to the most recent techniques available to veterinarians, the management of parturition, especially of C-sections, has become a topic of greater importance. Anesthesia is crucial and must be targeted to both the mother and neonates. The present study aimed to evaluate the effect of the induction agent alfaxalone on the vitality of puppies born from elective C-section, in comparison to propofol. After inducing general anesthesia for elective C-section, puppies from the mothers induced with alfaxalone had higher 5-min Apgar scores than those induced with propofol. The concentration of cortisol in fetal fluids collected at birth is neither influenced by the anesthetic protocol used, nor does it differ between amniotic and allantoic fluids. Nevertheless, the cortisol concentration in fetal fluids affects the relationship between anesthesia and the Apgar score: the present study highlights a significant relationship between the anesthetic protocol used and Apgar score in puppies, and fetal fluids cortisol concentration acts as a covariate of this relationship. Alfaxalone is a safe and effective drug for the induction of anesthesia in dogs, and it can be successfully employed for elective C-section, with neonatal wellbeing improvements
Immunogenetic profiles of mothers and maternal response of fetal MHC antigens
No full textWe studied the genetic predisposition of mothers to producing cytotoxic antibodies against the HLA antigens of the fetus and the biological significance of this phenomenon, by investigating 250 mothers and 30 repetitive aborters for HLA polymorphisms (class I, II, properdin factor B) and for immunoglobulin allotypes (Gm and Km). We subdivided the mothers, into antibody positive and antibody negative groups by testing their sera against a large panel of T and B enriched suspensions. None of the women who had miscarried produced anti-fetus cytotoxic antibodies. The frequency of the antigen DR3 in antibody negative mothers and of DR5 in antibody positive ones was increased. The typing of factor B (serum complement protein) demonstrated an association between the genetic predisposition to respond to fetal MHC antigens and the F variant
Network-guided modelling allows tumor-type independent prediction of sensitivity to all-trans retinoic acid
No full textBackground: All-trans-retinoic acid (ATRA) is a differentiating agent used in the treatment of acute-promyelocytic-leukemia (APL) and it is under-exploited in other malignancies despite its low systemic toxicity. A rational/personalized use of ATRA requires the development of predictive tools allowing identification of sensitive cancer types and responsive individuals.
Materials and methods: RNA-sequencing data for 10 080 patients and 33 different tumor types were derived from the TCGA and Leucegene datasets and completely re-processed. The study was carried out using machine learning methods and network analysis.
Results: We profiled a large panel of breast-cancer cell-lines for in vitro sensitivity to ATRA and exploited the associated basal gene-expression data to initially generate a model predicting ATRA-sensitivity in this disease. Starting from these results and using a network-guided approach, we developed a generalized model (ATRA-21) whose validity extends to tumor types other than breast cancer. ATRA-21 predictions correlate with experimentally determined sensitivity in a large panel of cell-lines representative of numerous tumor types. In patients, ATRA-21 correctly identifies APL as the most sensitive acute-myelogenous-leukemia subtype and indicates that uveal-melanoma and low-grade glioma are top-ranking diseases as for average predicted responsiveness to ATRA. There is a consistent number of tumor types for which higher ATRA-21 predictions are associated with better outcomes.
Conclusions: In summary, we generated a tumor-type independent ATRA-sensitivity predictor which consists of a restricted number of genes and has the potential to be applied in the clinics. Identification of the tumor types that are likely to be generally sensitive to the action of ATRA paves the way to the design of clinical studies in the context of these diseases. In addition, ATRA-21 may represent an important diagnostic tool for the selection of individual patients who may benefit from ATRA-based therapeutic strategies also in tumors characterized by lower average sensitivity
Shock-induced disordering of MgSiO3 orthoenstatite
No full textData for "Direct observation of shock-induced disordering of enstatite below the melting temperature" by J.-A. Hernandez, G. Morard, M. Guarguaglini, R. Alonso-Mori, A. Benuzzi-Mounaix, R. Bolis, G. Fiquet, E. Galtier, A. E. Gleason, S. Glenzer, F. Guyot, B. Ko, H. J. Lee, W. L. Mao, B. Nagler, N. Ozaki, A. K. Schuster, S. H. Shim, T. Vinci, and A. Ravasi
Shock-induced disordering of MgSiO3 orthoenstatite
No full textData for "Direct observation of shock-induced disordering of enstatite below the melting temperature" by J.-A. Hernandez, G. Morard, M. Guarguaglini, R. Alonso-Mori, A. Benuzzi-Mounaix, R. Bolis, G. Fiquet, E. Galtier, A. E. Gleason, S. Glenzer, F. Guyot, B. Ko, H. J. Lee, W. L. Mao, B. Nagler, N. Ozaki, A. K. Schuster, S. H. Shim, T. Vinci, and A. Ravasi
Shock-induced disordering of MgSiO3 orthoenstatite
No full textData for "Direct observation of shock-induced disordering of enstatite below the melting temperature" by J.-A. Hernandez, G. Morard, M. Guarguaglini, R. Alonso-Mori, A. Benuzzi-Mounaix, R. Bolis, G. Fiquet, E. Galtier, A. E. Gleason, S. Glenzer, F. Guyot, B. Ko, H. J. Lee, W. L. Mao, B. Nagler, N. Ozaki, A. K. Schuster, S. H. Shim, T. Vinci, and A. Ravasi
Laparoscopic aspiration of ovarian endometriomas : effect with postoperative gonadotropin releasing hormone agonist treatment
No full textIn the period 1988-1990 this prospective study of 33 women with moderate or severe endometriosis who underwent laparoscopy for infertility and/or chronic pelvic pain, was conducted to evaluate the efficacy of aspirating endometriotic cysts followed by administration of a gonadotropin releasing hormone (GnRH) agonist in reducing the size of ovarian endometriomas. The cysts (mean diameter, 4.5 cm; range, 2-7; unilateral, 21 cases; bilateral, 12 cases) were punctured, aspirated, washed and emptied completely. After laparoscopy, 15 subjects received goserelin administered as a 28-day subcutaneous depot for three months, whereas 18 patients undergoing simple observation constituted internal controls. Ultrasound scans were performed before and at one, three and six months after laparoscopy. One case and three controls requested surgery between the four- and five-month follow-up scans and did not complete the study. All the other women had recurrent cysts at the six-month scan. There were no significant differences in mean endometrioma diameter between the two groups at any observation time nor between prelaparoscopic and six-month ultrasound examinations within each treatment group. We conclude that aspiration and washing of endometriotic cysts, combined with postoperative administration of GnRH agonists or not, is ineffective
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