23 research outputs found
Proceedings of the 30th Annual Biochemical Engineering Symposium
No full textThe 30th Annual Biochemical Engineering Symposium was held on 15-16 September 2000 at the YMCA of the Rockies in Estes Park, under the sponsorship of the Department of Chemical Engineering of the University of Colorado, Boulder and the Colorado Institute for Research in Biotechnology. The symposium is devoted to presentations by students about their ongoing research. The program consisted of 10 oral presentations and 12 poster presentations. Because final publication of these works usually takes place elsewhere, the papers included in these proceedings are brief, and often cover works in progress.
Contents
Sequence and Phylogenetic Analysis of Phospholipase - D Maria Meisch, Alain Laederach, and Peter J. Reilly; Iowa State University
Designing a Molecular Model for the Cytochrome f-plastocyanin Molecular Interface: Cation-π Interactions Play an Important Role in the Molecular Recognition Event - Alain T. Laederach, Ekaterina V. Pletneva, and Nenad M. Kostic, Iowa State University
Overexpression of Secreted Reporter Protein by Mouse Mammary Tumor Virus Promoter: Effect of Glucocorticoid Receptor - Mangalampalli V. R. Murthy, Allison O'Brien, Rohaizah I. James, and Dhinakar S. Kompala; University of Colorad
Effect of Surface Properties of Proteins on the Growth Rate of Subtilisin Crystals - Xiaojing Pan and Charles E. Glatz; Iowa State University
Experimental Study of Phytodegradation Kinetics of Methyl Benzotriazole - Sigifredo Castro, Lawrence C. Davis, and Larry E. Erickson; Kansas State University
Process Simplification by Applying Expanded Bed Adsorption: A Case Study of Capturing ,8-Glucuronidase (rGUS) from Unclarified Transgenic Canola Extract - Y. Bai, A. D. Lit en, and C. E. Glatz
Integration of Membrane Protein Analysis into Model Development for the Biodegradation of Phenol and Toluene by Pseudomonas putida Fl - Valerie A. Pferdeort and Kenneth F. Reardon; Colorado State University
Modeling Blood Using Shear-thinning Fluids for Optimization of Blood Oxygenators - Carin M. Kahr and Ranil Wickramasinghe; Colorado State University
Comparative Ethanol Fermentation Performance of Xylose-Utilizing Zymomonas mobilis Strains in Mixed Glucose-Xylose Media - Qiang Gao and Dhinakar Kampala; University of Colorado; Min Zhang and James D. McMillan; National Renewal Energy Laboratory
Diffusion of MTBE through Alfalfa - Courtney R. Pitzer, Sigifredo Castro-Diaz, and L. C. Davis; Kansas State University
Vegetated Treatment of Vehicle Wash Sediments: A Field Demonstration - R. Karthikeyan, P. Kulakow, L. E. Erickson, and B. Leven; Kansas State University
Cellulase Recovery via Membrane Filtration and Sedimentation - Wendy D. Mores, Laura M. Fisher, and Robert H. Davis; University of Colorado
Polyelectrolyte Precipitation of Wild-type and Charge-modified β-Glucurondiase from Canola, Com and Soy Extracts - Todd J. Menkhaus, Sara U. Eriksson, Paul B. Whitson, and Charles E. Glatz; Iowa 86 State University
Oxidation-Enhanced Biodegradation of PAH- and PeP-Contaminated Soils - Shelley A. Allen and Kenneth F. Reardon; Colorado State University</p
Single use disposable BioSettler removes the dead cells and cell debris selectively to increase the viability percentage of mammalian cells (e.g., CAR-T) during expansion
No full textCurrent challenge in a FDA approved cell therapy product for adult B-cell leukemia is reported to be the percentage of viable cells after manufacturing is sometimes out of specified range. As the head of a large contract development and manufacturing organization observed, this fall in viability percentage during CAR-T cell manufacturing is a challenge to the whole industry.
We present a simple and powerful off-the-shelf solution to this big challenge in all mammalian cell culture expansion bioreactor system. Our single use disposable BioSettler has been demonstrated to be uniquely capable of removing dead cells and cell debris selectively from the bioreactor and returning or recycling live mammalian cells back to the expansion bioreactor. The mechanism of this very fine separation of dead cells from live cells is the exploitation of their vastly different sedimentation rates during enhanced sedimentation of live cells on inclined surfaces.
This inclined sedimentation technology has been proven extensively with Chinese hamster ovary (CHO) cells used in commercial manufacture of therapeutic antibodies. As the size and sedimentation velocity difference between live and dead cells are similar for CHO cells and CAR-T cells, our off-the-shelf BioSettler will be readily useful for removing the dead cells and cell debris from the rocking or Wave cell expansion bioreactor and increasing the percentage of viable CAR-T cells being expanded for adult cell therapy
Physiological responses to folate overproduction in lactobacillys plantarum WCFS1.
No full textAbstract Background Using a functional genomics approach we addressed the impact of folate overproduction on metabolite formation and gene expression in Lactobacillus plantarum WCFS1. We focused specifically on the mechanism that reduces growth rates in folate-overproducing cells. Results Metabolite formation and gene expression were determined in a folate-overproducing- and wild-type strain. Differential metabolomics analysis of intracellular metabolite pools indicated that the pool sizes of 18 metabolites differed significantly between these strains. The gene expression profile was determined for both strains in pH-regulated chemostat culture and batch culture. Apart from the expected overexpression of the 6 genes of the folate gene cluster, no other genes were found to be differentially expressed both in continuous and batch cultures. The discrepancy between the low transcriptome and metabolome response and the 25% growth rate reduction of the folate overproducing strain was further investigated. Folate production per se could be ruled out as a contributing factor, since in the absence of folate production the growth rate of the overproducer was also reduced by 25%. The higher metabolic costs for DNA and RNA biosynthesis in the folate overproducing strain were also ruled out. However, it was demonstrated that folate-specific mRNAs and proteins constitute 8% and 4% of the total mRNA and protein pool, respectively. Conclusion Folate overproduction leads to very little change in metabolite levels or overall transcript profile, while at the same time the growth rate is reduced drastically. This shows that Lactobacillus plantarum WCFS1 is unable to respond to this growth rate reduction, most likely because the growth-related transcripts and proteins are diluted by the enormous amount of gratuitous folate-related transcripts and proteins.</p
Investigation of bacterial growth on mixed substrates: Experimental evaluation of cybernetic models
No full textProceedings of the Twentieth Annual Biochemical Engineering Symposium
No full textThe 20th Annual Biochemical Engineering Symposium was held at Kansas State University on April 21,1990. The objectives of the symposium were to provide: (i) a forum for informal discussion of biochemical engineering research being conducted at the participating institutions and (ii) an opportunity for students to present and publish their work.
Twenty-eight papers presented at the symposium are included in this proceedings. Some of the papers describe the progress of ongoing projects, and others contain the results of completed projects. Only brief summaries are given of the papers that will be published in full elsewhere. The program of the symposium and a list of the participants are included in the proceedings.
ContentsCell Separations and Recycle Using an Inclined Settler, Ching-Yuan Lee, Robert H. Davis and Robert A. Sclafani
Micromixing and Metabolism in Bioreactors: Characterization of a 14 L Fermenter, K.S. Wenger and E.H. Dunlop
Production, Purification, and Hydrolysis Kinetics of Wild-Type and Mutant Glucoamylases from Aspergillus Awamori, Ufuk Bakir, Paul D. Oates, Hsiu-Mei Chen and Peter J. Reilly
Dynamic Modeling of the Immune System, Barry Vant-Hull and Dhinakar S. Kompala
Dynamic Modeling of Active Transport Across a Biological Cell: A Stochastic Approach, B.C. Shen, S.T. Chou, Y.Y. Chiu and L.T. Fan
Electrokinetic Isolation of Bacterial Vesicles and Ribosomes, Debra T.L. Hawker, Robert H. Davis, Paul W. Todd, and Robert Lawson
Application of Dynamic Programming for Fermentative Ethanol Production by Zymomonas mobilis, Sheyla L. Rivera and M. Nazmul Karim
Biodegradation of PCP by Pseudomonas cepacia, R. Rayavarapu, S.K. Banerji, and R.K. Bajpai
Modeling the Bioremediation of Contaminated Soil Aggregates: a Phenomenological Approach, S. Dhawan, L.E. Erickson and L.T. Fan
Biospecific Adsorption of Glucoamylase-I from Aspergillus niger on Raw Starch, Bipin K. Dalmia and Zivko L. Nikolov
Overexpression in Recombinant Mammalian Cells: Effect on Growth Rate and Genetic Instability, Jeffrey A. Kern and Dhinakar S. Kompala
Structured Mathematical Modeling of Xylose Fermentation, A.K. Hilaly, M.N. Karim, I. C. Linden and S. Lastick
A New Culture Medium for Carbon-limited Growth of Bacillus thuringiensis, W. -M. Liu and R.K. Bajpai
Determination of Sugars and Sugar Alcohols by High Performance Ion Chromatography, T. J. Paskach, H.-P. Lieker, P.J. Reilly, and K. Thielecke
Characterization of Poly-Asp Tailed B-Galactosidase, M.Q. Niederauer, C.E. Glatz, l.A. Suominen, C.F. Ford, and M.A. Rougvie
Computation of Conformations and Energies of cr-Glucosyl Disaccharides, Jing Zepg, Michael K. Dowd, and Peter J. Reilly
Pentachlorophenol Interactions with Soil, Shein-Ming Wei, Shankha K. Banerji, and Rakesh K. Bajpai
Oxygen Transfer to Viscous Liquid Media in Three-Phase Fluidized Beds of Floating Bubble Freakers, Y. Kang, L.T. Fan, B.T. Min and S.D. Kim
Studies on the Invitro Development of Chick Embryo, A. Venkatraman and T. Panda
The Evolution of a Silicone Based Phase-Separated Gravity-Independent Bioreactor, Peter E. Villeneuve and Eric H. Dunlop
Biodegradation of Diethyl Phthalate, Guorong Zhang, Kenneth F. Reardon and Vincent G. Murphy
Microcosm Treatability of Soil Contaminated with Petroleum Hydrocarbons, P. Tuitemwong, S. Dhawan, B.M. Sly, L.E. Erickson and J.R. Schlup</p
Impact of nurse-delivered community-based CD4 services on facilitating pre-ART care in rural South Africa
No full text
Proceedings of the Twenty-First Annual Biochemical Engineering Symposium
No full textThe 21st Annual Biochemical Engineering Symposium was held at Colorado State University on April 20, 1991. The primary goals of this symposium series are to provide an opportunity for students to present and publish their research work and to promote informal discussions on biochemical engineering research.
Contents High Density Fed-Batch Cultivation and Energy Metabolism of Bacillus thuringtensis; W.-M. Liu, V. Bihari, M. Starzak, and R.K. Bajpai
Influences of Medium Composition and Cultivation Conditions on Recombinant Protein Production by Bacillus subtilis; K. Park, P.M. Linzmaier, and K.F. Reardon
Characterization of a Foreign Gene Expression in a Recombinant T7 Expression System Infected with λ Phages; F. Miao and D.S. Kompala
Simulation of an Enzymatic Membrane System with Forced Periodic Supply of Substrate; N. Nakaiwa, M. Yashima, L.T. Fan, and T. Ohmori
Batch Extraction of Dilut Acids in a Hollow Fiber Module; D.G. O'Brien and C.E. Glatz
Evaluation of a New Electrophoretic Device for Protein Purification; M.-J. Juang and R.G. Harrison
Crossflow Microfiltration and Membrane Fouling for Yeast Cell Suspension; S. Redkar and R. Davis
Interaction of MBP-β-Galactosidase Fusion Protein with Starch; L. Taladriz and Z. Nikolov
Predicting the Solubility of Recombinant Proteins in Escherichia coli; D.L. Wilkinson and R.G. Harrison
Evolution of a Phase-Separated, Gravity-Independent Bioractor; P.E. Villeneuve and E.H. Dunlop
A Strategy for the Decontamination of Soils Containing Elevated Levels of PCP; S. Ghoshal, S. K. Banelji, and RK. Bajpai
Practical Considerations for Implementation of a Field Scale In-Situ Bioremediation Project; J.P. McDonald, CA Baldwin, and L.E. Erickson
Parametric Sensitivity Studies of Rhizopus oligosporus Solid Substrate Fermentation; J. Sargantanis, M.N. Karim, and V.G. Murphy, and RP. Tengerdy
Production of Acetyl-Xylan Esterase from Aspergillus niger; M.R Samara and J.C. Linden
Biological and Latex Particle Partitioning in Aqueous Two-Phase Systems; D.T.L. Hawker, RH. Davis, P.W. Todd, and R Lawson
Novel Bioreactor /Separator for Microbial Desulfurization of Coal; H. Gecol, RH. Davis, and J .R Mattoon
Effect of Plants and Trees on the Fate, Transport and Biodegradation of Contaminants in the Soil and Ground Water; W. Huang, E. Lee, J.F. Shimp, L.C. Davis, L.E. Erickson, and J.C. Tracy
Sound Production by Interfacial Effects in Airlift Reactors; J. Hua, T.-Y. Yiin, LA Glasgow, and L.E. Erickson
Soy Yogurt Fermentation of Rapid Hydration Hydrothermal Cooked Soy Milk; P. Tuitemwong, L.E. Erickson, and D.Y.C. Fung
Influence of Carbon Source on Pentachlorophenol Degradation by Phanerochaete chrysosportum in Soil; C.-Y.M. Hsieh, RK. Bajpai, and S.K. Banerji
Cellular Responses of Insect Cells Spodopiera frugiperda -9 to Hydrodynamic Stresses; P.L.-H. Yeh and RK. Bajpa1
A Mathematical Model for Ripening of Cheddar Cheese; J. Kim, M. Starzak, G.W. Preckshoi, and R.K. Bajpai</p
Proceedings of the Eighteenth Annual Biochemical Engineering Symposium
No full textThe eighteenth annual biochemical engineering symposium was held during April 22–23, 1988 at the YMCA of the Rockies conference center in Estes Park, Colorado, under the sponsorship of the University of Colorado. Previous symposia in this series have been hosted by Kansas State University (1st, 3rd, 5th, 9th, 12th, 16th), University of Nebraska-Lincoln (2nd, 4th), Iowa State University (6th, 7th, l0th, 13th, 17th), University of Missouri–Columbia (8th, 14th), and Colorado State University (11th, 15th). Next year's symposium is scheduled to be held at the University of Missouri-Columbia.
The symposia are devoted to talks by students about their ongoing research. Because final publication usually takes place elsewhere, the papers included in the proceedings are brief, and often cover work in progress.
ContentsApplications of mass spectrometers in biochemical engineeringJohn P. McDonald, Ayush Gupta, and Lourdes Taladriz, Kansas State University
Enzymatic hydrolysis of corn gluten proteinsJulie Hardwick; Iowa State University
Improved Acetone-Butanol Fermentation AnalysisZ. Buday; Colorado State University
On-Line State Identification for Batch FermentationD. A. Gee and W. F. Ramirez; University of Colorado
Role of Spargers in Air-Lift ReactorsPeter U. Sohn and Rakesh K. Bajpai; University of Missouri–Columbia
The Interaction of Microcarriers and Turbulence within an Airlift FermenterG. Travis Jones; Kansas State University
Oxygen Diffusion in the Inter-Fiber Gel/Cell Matrix of NMR-Compatible Hollow Fiber Bio-ReactorsS. L. Hanson, B. E. Dale, and R. J. Gillies; Colorado State University
Characterization of Ca-alginate Gel Beads FormationHorngtwu Su, Rakesh K. Bajpai, and George W. Preckshot; University of Missouri–Columbia
Metabolic Effects of Chloramphenicol Resistance in the Recombinant Host/Vector System: E. coli RRl [pBR329]William E. Bentley, Dana C. Andersen, Dhinakar S. Kompala, and Robert H. Davis; University of Colorado
Genetic Engineering of Beta-Galactosidase to Aid in Fermentation Product Recovery by Polyelectrolyte PrecipitationD. E. Parker, C. E. Glatz, J. Zhao, C. F. Ford, S. M. Gendel, and M. A. Rougvie; Iowa State University
Biodegradation of Organic Compounds in SoilLourdes Taladriz, L. E. Erickson, and L. T. Fan; Kansas State University
Effect of Dilution, pH and Nutrient Composition on the Biodegradation of Metalworking FluidsAyush Gupta, L. E. Erickson, and L. T. Fan; Kansas State University
Dissolved Hydrogen Correlation with Redox Potential in Acetone-Butanol FermentationXiangdong Zhou; Colorado State University
Modeling of Ensiling Fermentation of Sweet SorghumA. K. Hilaly; Colorado State University</p
In silico evolution of diauxic growth
No full textThe glucose effect is a well known phenomenon whereby cells, when presented with two different nutrients, show a diauxic growth pattern, i.e. an episode of exponential growth followed by a lag phase of reduced growth followed by a second phase of exponential growth. Diauxic growth is usually thought of as a an adaptation to maximise biomass production in an environment offering two or more carbon sources. While diauxic growth has been studied widely both experimentally and theoretically, the hypothesis that diauxic growth is a strategy to increase overall growth has remained an unconfirmed conjecture. Here, we present a minimal mathematical model of a bacterial nutrient uptake system and metabolism. We subject this model to artificial evolution to test under which conditions diauxic growth evolves. As a result, we find that, indeed, sequential uptake of nutrients emerges if there is competition for nutrients and the metabolism/uptake system is capacity limited. However, we also find that diauxic growth is a secondary effect of this system and that the speed-up of nutrient uptake is a much larger effect. Notably, this speed-up of nutrient uptake coincides with an overall reduction of efficiency. Our two main conclusions are: (i) Cells competing for the same nutrients evolve rapid but inefficient growth dynamics. (ii) In the deterministic models we use here no substantial lag-phase evolves. This suggests that the lag-phase is a consequence of stochastic gene expression
