1,238 research outputs found
sj-xlsx-3-pul-10.1177_20458940211031109 - Supplemental material for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension
No full textSupplemental material, sj-xlsx-3-pul-10.1177_20458940211031109 for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension by Ravikumar Sitapara, TuKiet T. Lam, Aneta Gandjeva, Rubin M. Tuder and Lawrence S. Zisman in Pulmonary Circulation</p
sj-pdf-4-pul-10.1177_20458940211031109 - Supplemental material for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension
No full textSupplemental material, sj-pdf-4-pul-10.1177_20458940211031109 for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension by Ravikumar Sitapara, TuKiet T. Lam, Aneta Gandjeva, Rubin M. Tuder and Lawrence S. Zisman in Pulmonary Circulation</p
sj-xlsx-2-pul-10.1177_20458940211031109 - Supplemental material for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension
No full textSupplemental material, sj-xlsx-2-pul-10.1177_20458940211031109 for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension by Ravikumar Sitapara, TuKiet T. Lam, Aneta Gandjeva, Rubin M. Tuder and Lawrence S. Zisman in Pulmonary Circulation</p
sj-pdf-1-pul-10.1177_20458940211031109 - Supplemental material for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension
No full textSupplemental material, sj-pdf-1-pul-10.1177_20458940211031109 for Phosphoproteomic analysis of lung tissue from patients with pulmonary arterial hypertension by Ravikumar Sitapara, TuKiet T. Lam, Aneta Gandjeva, Rubin M. Tuder and Lawrence S. Zisman in Pulmonary Circulation</p
Synthesis of meso-substituted core modified expanded porphyrins; Effect of acid catalysts on the cyclization
No full text[3+2] condensation between modified tripyrromethane and bithiophene or bifurandiol leads to the formation of 18π, 22π, 26π macrocycles under Lewis acid conditions while only 22π macrocycle is formed under protic acid conditions
A Liquid-Phase Process Suitable for Large-Scale Synthesis of Phosphorothioate Oligonucleotides
No full textORGANIC PROCESS RES. AND DEV
Programming Dendritic cells for intercellular delivery of T-bet to enhance function of cytotoxic T-cells
No full textAdvances in antiretroviral therapy (ART) have proven successful for controlling HIV-1 in chronically infected individuals. Despite these advancements, curing HIV-1 infection poses a major public health challenge due to the establishment and maintenance of HIV-1 latency in long lasting memory CD4+ T cells during ART. Moreover, the cytotoxic T cells (CTL) needed to effectively target and kill infected cells often become exhausted because of chronic activation. Interestingly, CTL from HIV elite controllers show less evidence exhaustion, which is also associated with higher levels of expression of the Th1-associated transcription factor T-bet. In this study, we hypothesize that type-1 polarized human dendritic cells (DC1) are superior in their capacity to induce and enhance cellular immune responses against virally infected cells partially due to their capacity to express and transfer DC-derived T-bet to effector T cells. Moreover, we propose that overexpression of T-bet in therapeutic DC, through genetic modification, offers another approach to improve DC-induced cellular immunity. Here we show that DC1 indeed uniquely express T bet as a general trait while conventional DC generated in the presence of PGE2 (DC2) are T-bet deficient as determined by western blot and intracellular flow cytometry analysis. We also report that overexpression of T-bet in DC1 (DC1Tbet) through use of an adenoviral vector delivery system enhances their CTL inducing activity. However, DC1Tbet display a reduction in their capacity to produce IL-12p70 upon activation with CD40L. Moreover, using a GFP-based tracking method, we demonstrate that DC1 have the capacity to directly transfer cytoplasmic content to activated CD8+ T cells in a CD40L dependent manner. These data suggest, both a novel helper function of CD40L expressing CD4+ Th cells, and a mechanism for potential DC to T cell transfer of T-bet. We propose that this immune mechanism of DC1 to CTL intercellular transfer can be exploited to enhance anti-HIV T cell response, or to correct their dysfunction of T cell exhaustion as supported by evidence in DC1-based cancer immunotherapy studies and help develop a better understanding of intercellular communication routes in both health and disease demonstrating the significance of this research in public health
A study on the dynamics of the zraP gene expression profile and its application to the construction of zinc adsorption bacteria
No full textZinc ion plays essential roles in biological chemistry. Bacteria acquire Zn(2+) from the environment, and cellular concentration levels are controlled by zinc homeostasis systems. In comparison with other homeostatic systems, the ZraSR two-component system was found to be more efficient in responding to exogenous zinc concentrations. To understand the dynamic response of the bacterium ZraSR two-component system with respect to exogenous zinc concentrations, the genetic circuit of the ZraSR system was integrated with a reporter protein. This study was helpful in the construction of an E. coli system that can display selective metal binding peptides on the surface of the cell in response to exogenous zinc. The engineered bacterial system for monitoring exogenous zinc was successfully employed to detect levels of zinc as low as 0.001 mM, which directly activates the expression of chimeric ompC(t)-zinc binding peptide gene to remove zinc by adsorbing a maximum of 163.6 mu mol of zinc per gram of dry cell weight. These results indicate that the engineered bacterial strain developed in the present study can sense the specific heavy metal and activates a cell surface display system that acts to remove the metal
Public Infrastructure Spillovers and Growth: Theory and Time Series Evidence for Australia.
No full textIn this paper, growth of per capita income can be exogenous and/or endogenous due to aggregate public infrastructure spillover. The deterministic Glomm and Ravikumar (1994) model is augmented in this paper to produce a stochastic growth counterpart which has useful time series implications.INCOME ; TIME SERIES ; CAPITAL
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