3 research outputs found
DNA Extraction from Pulp Tissue for its Application in PCR. A Study to Extract DNA with High Purity as Quantified by Gel Electrophoresis
No full textABSTRACT
The isolation of human genomic DNA is an important step involved in genetic identity analysis. Also getting optimal quantity and quality of DNA is important as this can alter the results. This consideration is especially important in the field of forensic analysis and genetic identity, where DNA is extracted from extremely small amounts of starting material often recovered from suboptimal storage conditions. Odontological examinations have always been a critical determinant in the search of human identity. Teeth form a natural barrier to exogenous DNA contamination. DNA extraction from dental tissues both hard tissues and dental pulp, has been reported the use of dental pulp, as a source of DNA offers a new paradigm in the process of DNA extraction and its use in forensic analysis.11 The following procedures elucidate a novel method of extraction of DNA using dental pulp, which offers a cost-effective and attractive procedure, which can be replicated and applied in DNA amplification (PCR).</jats:p
Epidemiological and Clinicopathological Analysis of 92 Odontogenic Tumors: A 5-year Retrospective Study
No full textABSTRACT
Introduction
Odontogenic tumors (OTs) are a heterogeneous group of lesions that are derived from odontogenic apparatus comprised of odontogenic epithelium, ectomesenchyme, and/or mesenchymal elements. The OTs show marked geographical variation. This study was conducted to analyze the epidemiology and clinicopathological presentation of OTs based on age, sex, and site.
Materials and methods
This study was conducted in Department of Oral Pathology and Microbiology. Records were reviewed retrospectively for all the lesions of oral cavity from January 2010 to December 2015. A total of 92 lesions were found to be OTs and were classified into benign and malignant tumors. They were further subdivided into three subtypes based on the types of odontogenic tissues involved. These were epithelial OTs (EOTs), mixed OTs (MIXOTs), and mesenchymal OTs (MOTs).
Results
Of 92 OTs, 84 were benign (males 48, females 36) and 8 were malignant (male 2, females 6). The most common benign tumor was ameloblastoma (AME) (20), followed by keratocystic OT (KCOT) (17), calcifying EOT (CEOT) (14), compound odontome (OD-Cd) (12), complex odontome (OD-Cx) (10), odontogenic fibroma (OF) (5), odontogenic myxoma (OM) (4), and cementoblastoma (CB) (2). The most common malignant tumor was primary intraosseous squamous cell carcinoma (PIOSCC) (3) followed by fibrosarcoma (FS) (3) and ameloblastic carcinoma (AC).
Conclusion
Author concluded that there was geographic and demographic variation in distribution of OTs, which may be attributed to socioeconomic and genetic factors.
Clinical significance
Literature showing prevalence of OTs in India is negligible. By this article, we have analyzed the frequency of various OTs according to sex, age, and site. A comprehensive record of OTs should be started so that pathologists and surgeons would be able to acquire the information about the tumor for reference in the future.
How to cite this article
Sharma I, Venkatesh D, Bawa G, Vaseemuddin S, Joseph A, Sangtani JK. Epidemiological and Clinicopathological Analysis of 92 Odontogenic Tumors: A 5-year Retrospective Study. J Contemp Dent Pract 2017;18(11):1056-1060.
</jats:sec
Various Shapes of Soft Palate: A Lateral Cephalometric Study
No full textABSTRACT
Objective
Though the soft palate presents with varied morphology, very few studies have described its diversity. The aim of our study was to investigate various shapes of soft palate in normal individuals.
Methods
The study comprised of 80 individuals requiring orthodontic treatment but without any speech abnormality, whose age ranged from 9 to 31 years. Velar shape was examined on digital lateral cephalograms and was allocated to one of the six patterns as described by You M et al. The difference in proportion of each type and also difference between genders were studied.
Results
The normal soft palate can be classified into six types based on its shape. Type 2—rat-tail shape is most common in both the genders. There is no significant difference in proportion of various shapes of soft palate between genders.
Conclusion
Soft palate may have variable morphology. Knowledge of varied spectrum of velar morphology may help in successful functional and structural repair in cleft palate cases, and shed some light towards the causes of obstructive sleep apnea and related disorders.
</jats:sec
