13 research outputs found

    OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO : Konsentrasi Optimal Dari Ekstrak Daun Kemangi (Ocimum Basilicum L.) Dalam Menghambat Pertumbuhan Bakteri Streptococcus Mutans In Vitro

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    Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro

    FRENECTOMY ON MAXILLARY LABIAL FRENULUM PENETRATING PAPILLA TYPE BY CONVENTIONAL SURGICAL TECHNIQUES: A CASE REPORT

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    Introduction: The frenulum is a fold of mucosa that attaches the lips and cheeks to the alveolar mucosa, gingiva, and underlying periosteum. If the attachment is too high and thick to approach the gingival margin (papilla penetrating type) it causes a central diastema on the upper incisor or recurrence after orthodontic treatment, besides causing oral hygiene problems and gingival recession as well as denture stability. An abnormal frenulum should be removed by frenectomy. History and Clinical Finding: An 18-year-old female patient with a tall and thick maxillary labial frenulum as a cause of central diastema on teeth 11, 21. The patient had been treated with removable orthodontics starting 2 years ago. Since 4-6 months ago the diastema has relapse. The maxillary labial frenulum appears to be attached to the incisive papilla (penetration of the papillae). The Blanch test shows movement of the interdental papillae and blanching (ischemia) of the incisor papillae. Case Management: Frenectomy using a scalpel until all the papillae and fibrous fibers are removed. Control 14 days postoperative, the wound was healed. During the blanching test, there was no movement of the papillae and the color was normally. Discussions: The high maxillary labial frenulum causes the two central incisors to erupt far apart. Orthodontic treatment  must be accompanied by removal of the etiological factor by frenectomy for the treatment to be successful. Conclusions: Penetrating papilla type frenectomy of the maxillary labial frenulum with the conventional technique of using a scalpel effectively eliminates the causes of central diastema

    Diffusion-weighted MR imaging of non-complicated hepatic cysts: Value of 3T computed diffusion-weighted imaging

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    AbstractPurposeTo investigate the utility of computed 3T diffusion-weighted imaging (c-DWI) for the diagnosis of non-complicated hepatic cysts with a focus on the T2 shine-through effect.Materials and methodsIn 50 patients with non-complicated hepatic cysts we acquired one set of DWIs (b-value 0 and 1000s/mm2) at 1.5T, and two sets at 3T (b-value 0 and 1000s/mm2, TE 70ms; b-value 0 and 600s/mm2, TE 60ms). We defined the original DWIs acquired with b=1000s/mm2 at 1.5T and 3T as “o-1.5T-1000” and “o-3T-1000”. c-DWIs were calculated with 3T DWI at b-values of 0 and 600s/mm2. c-DWI with b=1000 and 1500s/mm2 were defined as “c-1000” and “c-1500”. Radiologists evaluated the signal intensity (SI) of the cysts using a 3-point score where 1=not visible, 2=discernible, and 3=clearly visible. They calculated the contrast ratio (CR) between the cysts and the surrounding liver parenchyma on each DWIs and recorded the apparent diffusion coefficient (ADC) with a b-value=0 and 1000s/mm2 on 1.5T- and 3T DWIs.ResultsCompared with o-1.5T-1000 DWI, the visual scores of all but the c-1500 DWIs were higher (p=0.07 for c-1500- and p<0.01 for the other DWIs). The CR at b=1000s/mm2 was higher on o-3T-1000- than on o-1.5T-1000- (p<0.01) but not higher than on c-1500 DWIs (p=0.96). The CR at b=0s/mm2 on 3T images with TE 70ms was higher than on 1.5T images (p<0.01). The ADC value was higher for 3T- than 1.5T images (p<0.01).ConclusionsNon-complicated hepatic cysts showed higher SI on o-3T-1000- than o-1.5T-1000 DWIs due to the T2-shine through effect. This high SI was suppressed on c-1500 DWIs

    Antibacterial Activity of Bidara Arab Leaves (Ziziphus spina-christi L) Extract Against Gram-negative Anaerobic Subgingival Bacteria

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    Background: Gram-negative anaerobic subgingival bacteria play an important role in the initiation and progression of periodontitis. The secondary metabolites contained in Bidara Arab leaves (Ziziphus spina-christi L) have potential to be antibacterial and inhibited the growth of pathogenic bacterial. The purposed of this study to evaluated the antibacterial activity of Bidara Leaves extract against gram-negative anaerobic subgingival bacteria. Method: Experimental laboratories using Mueller hinton agar to determine the antibacterial activity of Bidara leaves extract 40% and 60% against Porphyromonas gingivalis, Aggregatibacter ctinomycetemcomitans, and Fusobacterium nucleatum, with a comparison of the negative control group ethanol 96% and tetracycline as positive control. The production of extracts through maseration techniques and the subsequent phytochemical testing through tube reaction test. Result: The results of phytochemical analysis identified the presence of flavonoids, tannins, saponins, steroids. The inhibition test showed that the Bidara leaves extract exhibited strong inhibition and a concentration of  60% was more effective than 40% against Gram-negative anaerobic subgingival bacteria including Porphyromonas gingivalis, Aggregatibacter ctinomycetemcomitans, and Fusobacterium nucleatum. Conclusion: The Bidara Arab leaves (Ziziphus spina-christi L) extract at concentration 60% was more effective than 40% against gram-negative anaerobic bacteri

    INHIBITORY POWER OF MAS BANANA PEEL EXTRACT (MUSA ACUMINATA LADY FINGER) ON THE GROWTH OF STAPHYLOCOCCUS AUREUS IN VITRO

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    Introduction: Various sources of infectious disease can be found in the oral cavity. Prevention efforts include brushing your teeth properly and regularly. Besides that, gargling with mouthwash is one of the best additional prevention methods. In the literature it is known that mas banana peels contain antibacterial ingredients. The aim of this research was to determine whether banana peel extract (Musa acuminata lady finger) was able to inhibit the growth of Staphylococcus aureus bacteria.  Material and Methods: Extracts of 25%, 50%, 75% concentration of dried mas banana peel were made, sterile distilled water as a negative control and 0.2% Chlorhexidine as a positive control. Staphylococcus aureus ATCC 25923 as many as 3 bacterial colonies, spread evenly on sterile Nutrient Agar (NA) media. The paper discs were dipped for ± 30 seconds into 25%, 50%, 75% gold banana peel extract, 0.2% Chlorhexidine as a positive control and sterile Aquades as a negative control then incubated in an incubator at 37°C for 24 hours. The inhibition zone (clear area around the paper disc) was measured with a caliper (accuracy 0.02mm). The data obtained were tested for normality and homogeneity to determine whether or not there was a difference in the inhibitory power of banana mas (Musa acuminata lady finger) peel extract against Staphylococcus aureus bacteria. Results and Discussion: The results showed that at a concentration of 75% there was an inhibitory power for the growth of Staphylococcus aureus bacteria of 6.32 mm. Mas banana peel extract (Musa acuminata lady finger) has antibacterial content in the form of flavonoids, alkaloids, saponins and tannins which are able to inhibit the growth of Staphylococcus aureus bacteria in vitro. Conclusion: Mas banana peel extract (Musa acuminata lady finger) can inhibit the growth of Staphylococcus aureus bacteria in vitro with moderate criteria

    Aesthetic Complex Management of Four Upper Anterior Teeth

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    Introduction: Anterior teeth require special attention in terms of aesthetics. Complex aesthetic treatment becomes the main choice in cases of anterior teeth with more than one caries, deformities, malposition and in needs of root canal treatment. Case: A 49-year-old female patient complained of broken upper right and left front teeth due to impact. Objective examination found that the lingual margins of teeth 11, 12 and 13 appeared reddish and bled easily, composite fillings with secondary caries at 21 dentin depths and 22 pulp depths, chipping at 11, 12 and 22. Percussion of tooth 22 (+), pressure test (-) and CE (+). Case Treatment: One visit root canal treatment in 22, direct composite veneer in 11, 21, 22 and composite restoration in 12. Discussions: Complex aesthetic treatment involves more than one anterior tooth that requires root canal treatment as well as correction of shape and position. Determination of shape, tooth proportion, color, position dimension, inclination, smile position and lip line, relationship between teeth, relationship of facial midline to lip midline are required in complex aesthetic treatment. Composite resin is chosen as a restorative material due to aesthetic considerations, faster procedure, low cost, does not take much of healthy tooth structure, easy to be repaired when there is damage and does not damage the opposing teeth. The use of palatal putty index can increase the efficiency of the work and maximize the final result. Conclusion and Suggestions: Complex aesthetic treatment using direct composite veneer is a fast, low-cost treatment option that can provide good aesthetic results in cases of four malformed and malpositioned upper anterior teeth

    Enhancing the resilience of drinking water infrastructures

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    Long term uncertainties in combination with long lifetime of assets of drinking water infrastructures (DWIs) and changing expectations of stakeholders make strategic decisions in drinking water infrastructures (DWIs) complex. A framework with building blocks and design spaces was developed to support these decisions. Building blocks, divided in governance and system properties, were generic resilience enhancing design principles found in literature. The design spaces were defined by characteristics (water quantity, water quality and environmental impact), and the scale dimension. The DWI design principles framework was operationalised in a case study. The case showed that the DWI design principles framework was useful for strategic issues and the results were recognised and accepted by a diverse group of stakeholders. It may also be possible to apply the framework for other water infrastructures with comparable characteristics and dimensions.Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Applied SciencesSanitary Engineerin

    Content of Volume 19 Issue 1 June 2023

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    Content of Volume 19 Issue 1 June 2023 SUB-ACUTE TOXICITY TEST OF RED GINGER EXTRACT (Zingiber officinale var. Rubrum) ON MICE (Mus musculus)Putu Sulistiawati Dewi, Mochammad Taha Ma’ruf1-5 THE EFFECT OF SMOKING HABITS ON THE OCCURRENCE OF BRUXISM: A RAPID REVIEWPrida Lani Debora P, Rasmi Rikmasari, Seto Pramudita6-10 TREATMENT OF HERPES ASSOCIATED ERYTHEMA MULTIFORME (HAEM)Raziv Ganesha, Rina Kartika Sari11-16 CORRELATION OF MOUTH BREATHING HABITS TO DENTAL MALOCCLUSIONSKetut Virtika Ayu, I Dewa Gede Budijanana, Norman Hidajah, Surwandi Walianto17-21 EARLY DETECTION OF VERRUCOUS CARCINOMA USING METHYLENE BLUE STAINING IN SUSPECTED LEUKOPLAKIA LESION: A CASE REPORTRina Kartika Sari, Raziv Ganesha, Esti Kurniawati22-28 RELATIONSHIP BETWEEN THE ANGLE OF INCLINATION OF THE MAXIMUM INCLINATION TO THE SOFT TISSUE OF THE FACIAL IN BALINESE WOMEN USING THE HOLDAWAY METHODNorman Hidajah, Dwis Syahrul, Ketut Virtika Ayu29-33 GEL GEL EXTRACTION OF EARTHWORMS (Lumbricus rubellus) TO THE NUMBER OF FIBROBAL CELLS IN MALE WISTAR RATS (Rattus norvegiccus) GINGIVAL WOUND HEALINGNi Wayan Arni Sardi, Ni Luh Putu Sri Maryuni Adnyasari, Ni Putu Ratih Berliana Ekasari34-42 THE USE OF CHLORHEXIDINE MOUTHWORKS CAN REDUCE THE ACCUMULATION OF DENTAL PLAK IN USERS OF FIXED ORTHODONTICDEVICESDwis Syahrul, Surwandi Walianto, Priscilla Sonia Suwongto43-48 EFFECTIVENESS OF RED BITES FRUIT (Beta vulgaris) AS A MOUTH MOUTH TO REDUCE HALITOSISI Gusti Ngurah Putra Dermawan, Intan Kemala Dewi, Fritz Gerald Dharma Tedjamartono49-54 PLAQUE CONTROL IN PERIODONTAL DISEASENi Luh Putu Sri Maryuni Adnyasari, Dwis Syahriel , I.G.A Dewi Haryani55-61 ERUPTION CYST OVERLYING THE CROWN OF UPPER RIGHT FIRST PREMOLAR: A CASE REPORTWayan Sutresna Yasa, Kirana Guspiari, I Made Hendra Cahyadita Nusantara Putra62-66 LD50 ACUTE TOXICITY TEST OF GREEN GRASS JELLY (Cyclea barbata Miers) LEAF EXTRACT AGAINST MICE (Mus musculus L.)Hendri Poernomo, Mochammad Taha Ma’ruf, Ari Sucitra Dewi67-7

    PLAQUE CONTROL IN PERIODONTAL DISEASE

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    Introduction: Periodontal disease results from a complex interplay between the subgingival biofilm and the host immune-immflamatory events that develop in the gingival and periodontal in response to the challenge presented by the bacteria. Gingivitis precedes periodontitis. In gingivitis , the inflammatory lesion is confined to the gingiva, however, with periodontits, the inflammatory processes extend to additionally affect the periodontal ligament and the alveolar bone. Review: The primary cause of gingival inflammattion is bacterial plaque. Other predisposing factors include calculus, faulty restoration complication associated with orthodontic therapy. Microbial plaque biofilm control is an effective way of treating and preventing gingivitis and is an essential part of all procedures involved in the treatment and prevention of periodontal diseases. Good supragingival biofilm control has also been shown to affect the growth and composition of subgingival plaque biofilm so that it favors a healthier microflora and reduces calculus formation. Carefully performed daily home plaque biofilm control, combined with frequent professionally delivered plaque biofilm and calculus removal, reduces the amount of supragingival biofilm, decreases the total number of microorganisms in moderately deep pockets, including furcation areas, and greatly reduces the quantity of periodontal pathogens. Chemical inhibitors of plaque biofilm and calculus that are incoporated in mouthwashes or dentifrices also play an important role in controlling microbial biofilms. Conclusion: Daily plaque biofilm control permits patients to assume responsibility for their own oral health every day

    THE EFFECTIVENESS OF PROBIOTIC LOZENGES LACTOBACILLUS REUTERI PRODENTIS IN INCREASING SALIVARY SECRETION : Efektivitas Tablet Hisap Probiotik Lactobacillus Reuteri Prodentis Terhadap Sekresi Saliva

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    Introduction: Indonesian people experience dental and oral health problemsas much as 57.6%, one of the dental and oral health problems that is oftentreated is periodontal disease. Alternative prevention of periodontal disease isby consuming probiotics (Lactobacillus reuteri). Probiotic (Lactobacillusreuteri) contains L. reuteri bacteria which inhibits periodontal and cariogenicpathogenic bacteria so that it is suspected to increase salivary flow rate. Objectives: to determine the effectiveness of probiotics (Lactobacillus reuteri) to increase salivary secretion. Materials and methods: this studyused an experimental approach with a pre-test and post-test design. The research subjects were 30 students of the Faculty of Dentistry, MahasaraswatiDenpasar University who met the research criteria. The sample was dividedinto two groups, namely the group that consumed the probiotic Interlac Pro-D lozenges and the group that consumed a placebo. The data taken werethe salivary flow rate, which was measured twice, before and after administration of the probiotic Interlac Pro-D lozenges and placebo. Dataanalysis: data is processed using paired t-test and independent t-test. Resultsand Discussion: The results showed that the average salivary secretion before consuming Interlac Pro-D probiotic tablets was 0.3073 ± 0.02492 ml/minute, increasing to 0.328 ± 0.03299 ml/minute, placebo increasing to 0.276± 0.02293 ml/minute to 0.2853 ± 0.0256 ml/minute. There was a differencein the effectiveness of increasing salivary secretion between the groups consuming the probiotic Interlac Pro-D tablets and placebo (p &lt;0.005). Conclusion: taking probiotic lozenges Interlac Pro-D (Lactobacillus reuteriprodentis) is effective in increasing salivary secretion which is beneficial forpreventive therapy of periodontal disease
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