1,228 research outputs found
An Interview with Cass R. Sunstein: Author of The World According to Star Wars
No full textThe guest editors of special issue 12, Jason W. Ellis and Sean Scanlan, interview Cass R. Sunstein, the Robert Walmsley University Professor at Harvard, where he is founder and director of the Program on Behavioral Economics and Public Policy. He is the author of many books, including the bestseller Nudge: Improving Decisions about Health, Wealth, and Happiness (with Richard H. Thaler). His 2016 book The World According to Star Wars attempts to understand the Star Wars universe in ten chapters through the lenses of Sunstein’s academic interests, namely: culture, sociology, psychology, behavioral science, and political science. The book is both personal and theoretical, practical and academic. It takes accurate measure of the genesis of the movies, the movies themselves, and briefly, but trenchantly, it examines concepts such as reputational cascades and speculates on what Star Wars can teach viewers about constitutional disputes
Open source bioimage informatics for cell biology
Full text linkSignificant technical advances in imaging, molecular biology and genomics have fueled a revolution in cell biology, in that the molecular and structural processes of the cell are now visualized and measured routinely. Driving much of this recent development has been the advent of computational tools for the acquisition, visualization, analysis and dissemination of these datasets. These tools collectively make up a new subfield of computational biology called bioimage informatics, which is facilitated by open source approaches. We discuss why open source tools for image informatics in cell biology are needed, some of the key general attributes of what make an open source imaging application successful, and point to opportunities for further operability that should greatly accelerate future cell biology discovery.</p
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The crowd funding services handbook ::raising the money you need to finance your business, project, or invention /
No full text"In The Crowdsource Funding Services Handbook, author Jason R. Rich offers a step-by-step overview of the various crowdsource funding services available on the Web, as well as the necessary documentation required to launch a successful crowdfunding campaign."-
Quantitative fluorescence microscopy and image deconvolution
No full textQuantitative imaging and image de-convolution have become standard techniques for the modern cell biologist because they can form the basis of an increasing number of assays for molecular function in a cellular context. There are two major types of de-convolution approaches—deblurring and restoration algorithms. Deblurring algorithms remove blur but treat a series of optical sections as individual two-dimensional entities and therefore sometimes mishandle blurred light. Restoration algorithms determine an object that, when convolved with the point-spread function of the microscope, could produce the image data. The advantages and disadvantages of these methods are discussed in this chapter. Image de-convolution in fluorescence microscopy has usually been applied to high-resolution imaging to improve contrast and thus detect small, dim objects that might otherwise be obscured. Their proper use demands some consideration of the imaging hardware, the acquisition process, fundamental aspects of photon detection, and image processing. This can prove daunting for some cell biologists, but the power of these techniques has been proven many times in the works cited in the chapter and elsewhere. Their usage is now well defined, so they can be incorporated into the capabilities of most laboratories
At the (Kineto)chore, Yeast Really Are Like People
No full textProper chromosome segregation depends on correct attachments between microtubules and kinetochores. Budding yeast have been thought to achieve these attachments with different kinetics than other eukaryotes. Now, deploying specialized data processing techniques to achieve super-resolution images, Marco et al. demonstrate that this tractable cell-cycle model system shares more similarities with plants and animals than previously thought
Neotectonic map of the Cascadia margin
Full text linkby Chris Goldfinger, Jeffrey Beeson, Chris Romsos and Jason R. Patton.This archived document is maintained by the State Library of Oregon as part of the Oregon Documents Depository Program. It is for informational purposes and may not be suitable for legal purposes.Includes bibliographical references (pages 15-20).Mode of access: Internet from the Oregon Government Publications Collection.Text in English
Building a scientific data grid with DIGS
No full textWe provide an insight into the challenge of building and supporting a scientific data infrastructure with reference to our experience working with scientists from computational particle physics and molecular biology.We illustrate how, with modern high-performance computing resources, even small scientific groups can generate huge volumes (petabytes) of valuable scientific data and explain how grid technology can be used to manage, publish, share and curate these data. We describe the DiGS software application, which we have developed to meet the needs of smaller communities and we have highlighted the key elements of its functionality
Bod1, a novel kinetochore protein required for chromosome biorientation
Full text linkWe have combined the proteomic analysis of Xenopus laevis in vitro–assembled chromosomes with RNA interference and live cell imaging in HeLa cells to identify novel factors required for proper chromosome segregation. The first of these is Bod1, a protein conserved throughout metazoans that associates with a large macromolecular complex and localizes with kinetochores and spindle poles during mitosis. Small interfering RNA depletion of Bod1 in HeLa cells produces elongated mitotic spindles with severe biorientation defects. Bod1-depleted cells form syntelic attachments that can oscillate and generate enough force to separate sister kinetochores, suggesting that microtubule–kinetochore interactions were intact. Releasing Bod1-depleted cells from a monastrol block increases the frequency of syntelic attachments and the number of cells displaying biorientation defects. Bod1 depletion does not affect the activity or localization of Aurora B but does cause mislocalization of the microtubule depolymerase mitotic centromere- associated kinesin and prevents its efficient phosphorylation by Aurora B. Therefore, Bod1 is a novel kinetochore protein that is required for the detection or resolution of syntelic attachments in mitotic spindles. © 2007 Porter et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0</p
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