27,852 research outputs found
Time-series of HXT-GFP expression in budding yeast
No full textCells can match expression of a series of genes to a signal, so that each gene expresses in a particular range of the signal. An example is glucose transport in budding yeast where cells express different hexose-transporter (\g{HXT}) genes in different ranges of concentrations of extracellular glucose. Here we determine mechanistically how this matching to glucose occurs using time-lapse microscopy, microfluidics, dynamic glucose inputs, and mathematical modelling. We show that the glucose-sensing network works to generate a push-pull system of two pairs of repressors: as glucose rises, cells `pull' repression by two regulators, Mth1 and Std1, decreasing their activity while `pushing' repression by two others, Mig1 and Mig2; in falling glucose, cells reverse the push-pull. The combined activity of these repressors is an intracellular readout of extracellular glucose. Cells express those transporters with an affinity matching the current glucose concentration because the HXT promoters couple to the repressors in three principal ways, each specific to either low, medium, or high-affinity transporters. Through this regulation, budding yeast likely excels at importing glucose rapidly. Matching gene expression to a pattern of input is fundamental, and we believe that push-pull repression is likely widespread.Please see README.tx
Author Peter FitzSimons speaking at the National Library of Australia, Canberra, 13 November 2012 /
No full textTitle from acquisitions documentation.; Part of the collection: Portraits of author Peter FitzSimons speaking at the National Library of Australia, Canberra, 13 November 2012.; Acquired in digital format; access copy available online.; Mode of access: Online.; Photographed by a staff member of the National Library of Australia
Harry Swain with his grandson Thomas, residents on the island of Tristan da Cunha
No full textHarry Swain with his grandson Thomas Swain, residents on the island of Tristan da Cunha. (1964-1965) [Photo by Peter A. Munch
Gordon Glass and Margaret Swain sitting together during a meal
No full textGordon Glass and Margaret Swain, residents on the island of Tristan da Cunha, sitting together during a meal. (1964-1965) [Photo by Peter A. Munch
Fred Swain sits outside a house dressed in formal wear
No full textFred Swain, a resident on the island of Tristan da Cunha, sits outside a house dressed in formal wear, likely for a wedding. (1964-1965) [Photo by Peter A. Munch
Dillys Swain, a resident on the island of Tristan da Cunha
No full textDillys Swain, a resident on the island of Tristan da Cunha. (1964-1965) [Photo by Peter A. Munch
Mary Swain, a resident on the island of Tristan da Cunha
No full textMary Swain, a resident on the island of Tristan da Cunha. (1964-1965) [Photo by Peter A. Munch
Julian Swain, a resident on the island of Tristan da Cunha
No full textJulian Swain, a resident on the island of Tristan da Cunha. (1964-1965) [Photo by Peter A. Munch
Jeanette and Dillys Swain, residents on the island of Tristan da Cunha
No full textJeanette and Dillys Swain, residents on the island of Tristan da Cunha. (1964-1965) [Photo by Peter A. Munch
Thomas Swain, a resident on the island of Tristan da Cunha
No full textThomas Swain, a resident on the island of Tristan da Cunha. (1964-1965) [Photo by Peter A. Munch
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